Comprehensive Investigation of Juniperus virginiana Essential Oil: GC/MS Analysis, Thermoanalytical Characterization, and Evaluation of Bioactive Potential
Abstract
Due to the ever-increasing unregulated antibiotic use, there have been increased risks of antibiotic-resistant bacterial infections. Natural agents, their products, and derivatives have been used for centuries to cure a variety of ailments. In the present study, an attempt was made to explore the physiochemical properties of the CEO. GC-MS analysis was used to identify the volatile components present in the oil. Additionally, TGA and DSC analysis were carried out to understand the thermal properties of the oil. The oil was characterized by higher amounts of terpenoids. The essential oil has potent anticancer, cytotoxic, antimalarial, and anti-inflammatory activity. This study concludes that the Juniperus virginiana essential oil could be used as a therapeutic agent.
Keywords: Anticancer, Antioxidant, Cedarwood, Cytotoxicity, Essential oil
Keywords:
Anticancer, Antioxidant, Cedarwood, Cytotoxicity, Essential oil, Anticancer, Antioxidant, Cedarwood, Cytotoxicity, Essential oilDOI
https://doi.org/10.22270/jddt.v14i2.6368References
Hudzicki J. Kirby-Bauer Disk Diffusion Susceptibility Test Protocol. 2016;(December 2009):1-23.
Rossiter SE, Fletcher MH, Wuest WM. Natural Products as Platforms to Overcome Antibiotic Resistance. Vol. 117, Chemical Reviews. American Chemical Society; 2017. p. 12415-74. https://doi.org/10.1021/acs.chemrev.7b00283 PMid:28953368 PMCid:PMC5869711
Cohen ML. Epidemiology of drug resistance: Implications for a post-antimicrobial era. Science. 1992;257(5073):1050-5. https://doi.org/10.1126/science.257.5073.1050 PMid:1509255
Khan HA, Ahmad A, Mehboob R. Nosocomial infections and their control strategies. Asian Pacific Journal of Tropical Biomedicine. 2015;5(7):509-14. https://doi.org/10.1016/j.apjtb.2015.05.001
Antimicrobial resistance [Internet]. 2021. Available from: https://www.who.int/news-room/fact-sheets/detail/antimicrobial-resistance
Hutchings M, Truman A, Wilkinson B. Antibiotics: past, present and future. Current Opinion in Microbiology. 2019;51:72-80. https://doi.org/10.1016/j.mib.2019.10.008 PMid:31733401
Fisher K, Phillips C. Potential antimicrobial uses of essential oils in food: is citrus the answer? Trends in Food Science and Technology. 2008;19(3):156-64. https://doi.org/10.1016/j.tifs.2007.11.006
Chouhan S, Sharma K, Guleria S. Antimicrobial Activity of Some Essential Oils-Present Status and Future Perspectives. Medicines. 2017;4(3):58. https://doi.org/10.3390/medicines4030058 PMid:28930272 PMCid:PMC5622393
Kaloustian J, Chevalier J, Mikail C, Martino M, Abou L, Vergnes MF. Étude de six huiles essentielles: Composition chimique et activité antibactérienne. Phytotherapie. 2008;6(3):160-4. https://doi.org/10.1007/s10298-008-0307-1
Burt S. Essential oils: Their antibacterial properties and potential applications in foods - A review. International Journal of Food Microbiology. 2004;94(3):223-53. https://doi.org/10.1016/j.ijfoodmicro.2004.03.022 PMid:15246235
Jacobson M. Plants, insects, and man-their interrelationships. Economic Botany. 1982;36(3):346-54. https://doi.org/10.1007/BF02858560
Zrira S, Ghanmi M. Chemical Composition and Antibacterial Activity of the Essential of Cedrus atlantica (Cedarwood oil). Journal of Essential Oil-Bearing Plants. 2016;19(5):1267-72. https://doi.org/10.1080/0972060X.2015.1137499
Lucas A. Cosmetics, Perfumes and Incense in Ancient Egypt. The Journal of Egyptian Archaeology. 1930;16(1/2):41. https://doi.org/10.2307/3854332
Perry RD, Fetherston JD. Yersinia pestis - Etiologic agent of plague. Clinical Microbiology Reviews. 1997;10(1):35-66. https://doi.org/10.1128/CMR.10.1.35 PMid:8993858 PMCid:PMC172914
Chun S, Muthu M, Gansukh E, Thalappil P, Gopal J. The ethanopharmacological aspect of carbon nanodots in turmeric smoke. Scientific Reports. 2016;6(May):1-12. https://doi.org/10.1038/srep35586 PMid:27805007 PMCid:PMC5090208
Jacobson M. Plants, insects, and man-their interrelationships. Economic Botany. 1982;36(3):346-54. https://doi.org/10.1007/BF02858560
Hazra A, Alexander K, Dollimore D, Riga A. Characterization of some essential oils and their key components. Thermoanalytical techniques. Journal of Thermal Analysis and Calorimetry. 2004;75(1):317-30. https://doi.org/10.1023/B:JTAN.0000017352.86803.6d
Sokovicx́ M, Glamočlija J, Marin PD, Brkić D, Van Griensven LJLD. Antibacterial effects of the essential oils of commonly consumed medicinal herbs using an in vitro model. Molecules. 2010;15(11):7532-46. https://doi.org/10.3390/molecules15117532 PMid:21030907 PMCid:PMC6259430
Perczak A, Gwiazdowska D, Marchwińska K, Juś K, Gwiazdowski R, Waśkiewicz A. Antifungal activity of selected essential oils against Fusarium culmorum and F. graminearum and their secondary metabolites in wheat seeds. Archives of Microbiology. 2019;201(8):1085-97. https://doi.org/10.1007/s00203-019-01673-5 PMid:31123790 PMCid:PMC6746685
Kumar A, Suravajhala R, Bhagat M. Bioactive potential of Cedrus deodara (Roxb.) Loud essential oil (bark) against Curvularia lunata and molecular docking studies. SN Applied Sciences. 2020;2(6):1-9. https://doi.org/10.1007/s42452-020-2837-6
Semen E, Hiziroglu S. Production , Yield and Derivatives of Volatile Oils from Eastern Redcedar ( Juniperus Virginiana L .) Elif Semen and 2 Salim Hiziroglu Department of Forest Products Engineering , Faculty of Forestry Department of Forestry , Oklahoma State University , Sti. 2005;1(2):133-8. https://doi.org/10.3844/ajessp.2005.133.138
Stewart CD, Jones CD, Setzer WN. Essential oil compositions of Juniperus virginiana and Pinus virginiana, two important trees in Cherokee traditional medicine. ~ 17 ~ American Journal of Essential Oils and Natural Products. 2014;2(2):17-24.
Jeong HU, Kwon SS, Kong TY, Kim JH, Lee HS. Inhibitory effects of cedrol, β-cedrene, and thujopsene on cytochrome P450 enzyme activities in human liver microsomes. Journal of Toxicology and Environmental Health - Part A: Current Issues. 2014;77(November 2014):1522-32. https://doi.org/10.1080/15287394.2014.955906 PMid:25343299
Cedarwood Essential Oil: Harness The Incredible Benefits Of This Earthy Tincture [Internet]. 2022. Available from: https://www.netmeds.com/health-library/post/cedarwood-essential-oil-harness-the-incredible-benefits-of-this-earthy-tincture
Wang YH, Zhang YR. Variations in compositions and antioxidant activities of essential oils from leaves of Luodian Blumea balsamifera from different harvest times in China. PLoS ONE. 2020;15(6):1-15. https://doi.org/10.1371/journal.pone.0234661 PMid:32544201 PMCid:PMC7297349
Wiley Registry of Mass Spectral Data, 12th Edition. Wiley Science Solutions; 2017.
Jaswant Singh B, Bhattacharji LM, Dtm M. Rapid Staining of Malarial Parasites by a Water Soluble Stain. Ind Med Gaz. 1944 Mar;79(3):102-104.
Chelsey D. Stewart CDJ and WNS. Essential oil composition and antioxidant and antimicrobial properties of the aerial parts of Salvia eremophila Boiss. from Iran. Food and Chemical Toxicology, 48(5), 1371-1376 |https://doi.org/10.1016/j.fct.2010.03.003 PMid:20211675
Narayanan BL, Kannappan K, Subburaju T, Sajeeth CI. GC-MS Analysis and HPTLC Fingerprinting Profile of Hydroalcoholic Extract of Polygonum barbatum Linn. Leaves. Indian Journal of Pharmaceutical Sciences. 2017;79(3). https://doi.org/10.4172/pharmaceutical-sciences.1000253
Florentine Marie-Chantal Ndoye Foe, Tatiana Flore Kemegni Tchinang AMN, Jean-Pierre Abdou, Abel Joel Gbaweng Yaya, Alembert Tiabou Tchinda, Jean-Louis Oyono Essame Etoa FX. Chemical composition, in vitro antioxidant and anti-inflammatory properties of essential oils of four dietary and medicinal plants from Cameroon, BMC Complementary and Alternative Medicine. 2016; 16(1):116-117. https://doi.org/10.1186/s12906-016-1096-y PMid:27056828 PMCid:PMC4823886
R&D F& FI. What are the Properties and Uses of Borneol [Internet]. 2021. Available from: https://foreverest.cn/news-list/what-are-the-properties-and-uses-of-borneol
Chaudhary A, Sood S, Das P, Kaur P, Mahajan I, Gulati A, Singh B. Synthesis of novel antimicrobial aryl himachalene derivatives from naturally occurring himachalenes. EXCLI J. 2014;13:1216-25.
Paek SH, Kim GJ, Jeong HS, Yum SK. Ar-turmerone and β-atlantone induce internucleosomal DNA fragmentation associated with programmed cell death in human myeloid leukemia HL-60 cells. Archives of Pharmacal Research. 1996 Apr;19(2):91-4. https://doi.org/10.1007/BF02976840
Chambre DR, Moisa C, Lupitu A, Copolovici L, Pop G, Copolovici DM. Chemical composition, antioxidant capacity, and thermal behavior of Satureja hortensis essential oil. Scientific Reports. 2020;10(1):1-12. https://doi.org/10.1038/s41598-020-78263-9 PMid:33288856 PMCid:PMC7721874
Mota ML, Lobo LTC, Galberto Da Costa JM, Costa LS, Rocha HAO, Rocha E Silva LF, et al. In vitro and in vivo antimalarial activity of essential oils and chemical components from three medicinal plants found in Northeastern Brazil. Planta Medica. 2012;78(7):658-64. https://doi.org/10.1055/s-0031-1298333 PMid:22441836
Gaínza YA, Domingues LF, Perez OP, Rabelo MD, López ER, Chagas AC de S. Anthelmintic activity in vitro of Citrus sinensis and Melaleuca quinquenervia essential oil from Cuba on Haemonchus contortus. Industrial Crops and Products. 2015;76:647-52. https://doi.org/10.1016/j.indcrop.2015.07.056
Braga MEM, Leal PF, Carvalho JE, Meireles MAA. Comparison of Yield, Composition, and Antioxidant Activity of Turmeric (Curcuma longa L.) Extracts Obtained Using Various Techniques. Journal of Agricultural and Food Chemistry. 2003;51(22):6604-11. https://doi.org/10.1021/jf0345550 PMid:14558784
Jan S, Khan MR, Rashid U, Bokhari J. Assessment of Antioxidant Potential, Total Phenolics and Flavonoids of Different Solvent Fractions of Monotheca Buxifolia Fruit. Osong Public Health and Research Perspectives. 2013;4(5):246-54. https://doi.org/10.1016/j.phrp.2013.09.003 PMid:24298440 PMCid:PMC3845226
Russo R, Corasaniti MT, Bagetta G, Morrone LA. Exploitation of cytotoxicity of some essential oils for translation in cancer therapy. Evidence-based Complementary and Alternative Medicine. 2015;2015. https://doi.org/10.1155/2015/397821 PMid:25722735 PMCid:PMC4334976
Sharma M, Grewal K, Jandrotia R, Batish DR, Singh HP, Kohli RK. Essential oils as anticancer agents: Potential role in malignancies, drug delivery mechanisms, and immune system enhancement. Biomedicine and Pharmacotherapy. 2022;146:112514. https://doi.org/10.1016/j.biopha.2021.112514 PMid:34963087
Amr E. Pharmaceutical and Therapeutic Potentials of Essential Oils and Their Individual Volatile Constituents: A Review. Phytotherapy research : PTR. 2007;21:308-323. https://doi.org/10.1002/ptr.2072 PMid:17199238
Mohamed TA, Albadry HA, Elshamy AI, Younes SHH, Shahat AA, El-wassimy MT, et al. A new Tetrahydrofuran sesquiterpene skeleton from Artemisia sieberi. Journal of the Chinese Chemical Society. 2021;68(2):338-42. https://doi.org/10.1002/jccs.202000198
Xie ZQ, Ding LF, Wang DS, Nie W, Liu JX, Qin J, et al. Sesquiterpenes from the Leaves of Magnolia delavayi Franch. and Their Cytotoxic Activities. Chemistry and Biodiversity. 2019;16(5). https://doi.org/10.1002/cbdv.201900013 PMid:30811806
Blowman K, Magalhães M, Lemos MFL, Cabral C, Pires IM. Anticancer Properties of Essential Oils and Other Natural Products. Evidence-based Complementary and Alternative Medicine. 2018;2018. https://doi.org/10.1155/2018/3149362 PMid:29765461 PMCid:PMC5889900
Elgamal AM, Ahmed RF, Abd-Elgawad AM, El Gendy AENG, Elshamy AI, Nassar MI. Chemical profiles, anticancer, and anti-aging activities of essential oils of pluchea dioscoridis (L.) dc. and erigeron bonariensis l. Plants. 2021;10(4):1-16. https://doi.org/10.3390/plants10040667 PMid:33807147 PMCid:PMC8066341
Do TKT, Hadji-Minaglou F, Antoniotti S, Fernandez X. Authenticity of essential oils. TrAC - Trends in Analytical Chemistry. 2015;66:146-57. https://doi.org/10.1016/j.trac.2014.10.007
Naik AV, Sellappan K. Chromatographic Fingerprint of Essential Oils in Plant Organs of Annona muricata L. (Annonaceae) using HPTLC. Analytical Chemistry Letters. 2020 Mar 3;10(2):214-26. https://doi.org/10.1080/22297928.2020.1763197
Alqarni MH, Foudah AI, Alam A, Salkini MA, Alam P, Yusufoglu HS. Novel HPTLC-densitometric method for concurrent quantification of linalool and thymol in essential oils. Arabian Journal of Chemistry. 2021 Feb;14(2):102916. https://doi.org/10.1016/j.arabjc.2020.102916
Romero Rocamora C, Ramasamy K, Meng Lim S, Majeed ABA, Agatonovic-Kustrin S. HPTLC based approach for bioassay-guided evaluation of antidiabetic and neuroprotective effects of eight essential oils of the Lamiaceae family plants. Journal of Pharmaceutical and Biomedical Analysis. 2020;178. https://doi.org/10.1016/j.jpba.2019.112909 PMid:31618702
Miguel MG. Antioxidant and anti-inflammatory activities of essential oils: A short review. Molecules. 2010;15(12):9252-87. https://doi.org/10.3390/molecules15129252 PMid:21160452 PMCid:PMC6259136
Dharmadeva S, Galgamuwa L, Prasadinie C KN. In vitro anti-inflammatory activity of Ficus racemosa L. bark using albumin denaturation method. AYU (An International Quarterly Journal of Research in Ayurveda); 2018. p. 39:239. https://doi.org/10.4103/ayu.AYU_27_18 PMid:31367147 PMCid:PMC6639822
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