Methanolic and ethanolic phytochemical screening of Sweet flag (Acorus calamus L.) rhizome
Abstract
Acorus calamus is a medicinal plant with several ethno-medicinal properties. The present study was carried to screen the presence of major phytochemical groups. Phytochemical screening of methanolic and ethanolic rhizome extract showed the presence of carbohydrates, alkaloids, phenolic compound, protein, amino acid flavanoids and tannins. The total phenolic content in methanolic and ethanolic rhizome extracts in terms of gallic acid equivalent was 4.77.50 and 7.5 mg/g of extract powder respectively whereas the total flavonoid content of methanolic and ethanolic extract was 12.02 and 12.89 mg QE/g of extract respectively. Total antioxidant activity was found highest in ethanol extract (75.6 µg AAE/mg of extract) than methanol extract (37.2 µg AAE/mg of extract). Reducing power is also found highest in ethanol extract than methanol extract.
Keywords: Acorus calamus, phytochemical screening, methanol, ethanol.
Keywords:
Acorus calamus, phytochemical screening, methanol, ethanol
Downloads
Download data is not yet available.
References
1. Ncube NS, Afolayan AJ, Okoh AI. Assessment techniques of antimicrobial properties of natural compounds of plant origin: current methods and future trends. African Journal of Biotechnology 2008; 7(12):1797-1806. https://doi.org/10.5897/AJB07.613
2. Hill AF. Economic botany a text book of useful plants and plant products. 2nd edn. Mcgraw-Hill book company Inc, NY. 1952.
3. Ramakrishna Y, Manohor AI, Mamata P, Shreekant KG. Plants and novel antitumor agents: A review. Indian Drug. 1984; 21:173-85.
4. Balakumbahan R, Rajamani K, Kumanan K. Acorus calamus: An overview. Jour Med Plants Res. 2010; 4: 2740-2745.
5. Copping LG. Crop protection agents from nature. In: Natural products and analogins: The Royal Society of Chemistry, Cambridge, 1996, 501.
6. Cavazza G. Les chimiotypes parmi les plantes aromatiques cas du calamus polyploide. Ann Fals Exp Chins, 1976; 69:833-844.
7. Archana Parki, Pinky Chaubey, Om Prakash, Ravendra Kumar and Anil K. Pant. Seasonal Variation in Essential Oil Compositions and Antioxidant Properties of Acorus calamus L. Accessions. Medicines, 2017; 4:81. https://doi.org/10.3390/medicines4040081 PMid:29117116 PMCid:PMC5750605
8. Kaufman PB, Cseke LJ, Warber S, Duke JA, Brielmann, Natural products from plants, CRC press, New York, 1999, pp. 20-22. https://doi.org/10.1201/9780849331343
9. Braca A, Tommasi ND, Bari LD, Pizza C, Politi M, Morelli I, J Nat Prod, 2001; 64:892-895. https://doi.org/10.1021/np0100845 PMid:11473417
10. Oyaizu M. Studies on products of browning reactions: antioxidant activities of products of browning reaction prepared from glucosamine. J Nutrit; 1986; 44:307-15. https://doi.org/10.5264/eiyogakuzashi.44.307
11. Prieto P, Pineda M, Aguilar M. Spectrophotometric Quantitation of Antioxidant Capacity through the Formation of a Phosphomolybdenum Complex: Specific Application to the Determination of Vitamin E1. Analytical Biochem, 1999; 269:337- 341 https://doi.org/10.1006/abio.1999.4019 PMid:10222007
12. Nanda B L, Sayeeda Nigar Sultana G and Thulasi T Radhakrishnan.Determination of phytochemicals and antioxidant activity of acorus calamus rhizome. Journal of Drug Delivery & Therapeutics; 2014; 4(6):117-121 https://doi.org/10.22270/jddt.v4i6.1005
13. Amit Bahukhandi, Sandeep Rawat, Indra D. Bhatt, Ranbeer S. Rawal . Influence of Solvent Types and Source of Collection on Total Phenolic Content and Antioxidant Activities of Acorus calamus L. Natl. Acad. Sci. Lett, January-February 2013; 36(1):93-99. https://doi.org/10.1007/s40009-012-0109-8
14. G. S. Suhartati Djarkasi, Lana E. Lalujan, Erny Nurali and Maria F. Antioxidant Activity of Karimenga (Acorus calamus). AIP Conference Proceedings 2019; 2155:020051. https://doi.org/10.1063/1.5125555
15. G.C. Bag, P. Grihanjali Devi, Th. Bhaigyabati. Phytochemical Screening and Antioxidant Activity of Meyna laxiflora Species Found in Imphal West District of Manipur. Int. J. Pharm. Sci. Rev. Res., January - February 2016, 36(1):137-143
16. Olayinka A, Aiyegoro AI, Okoh. Preliminary phytochemical screening and in vitro antioxidant activities of the aqueous extract of Helichrysum longifolium DC, BMC Complementary and Alternative Medicine, Oxford University Press, 2010; 10:21. https://doi.org/10.1186/1472-6882-10-21 PMid:20470421 PMCid:PMC2877649
17. Oktay M, Gulcin I, Kufrevioglu OI. Determination of in vitro antioxidant activity of fennel (Foeniculum vulgare) seed extracts, Lebensum. Wiss. U. Technol, 2003; 36:263-271. https://doi.org/10.1016/S0023-6438(02)00226-8
18. Chanda S, Dave R . In vitro models for antioxidant activity evaluation and some medicinal plants possessing antioxidant properties: An overview, Afr J Microbiol Res., 2009; 3(13):981-996.
2. Hill AF. Economic botany a text book of useful plants and plant products. 2nd edn. Mcgraw-Hill book company Inc, NY. 1952.
3. Ramakrishna Y, Manohor AI, Mamata P, Shreekant KG. Plants and novel antitumor agents: A review. Indian Drug. 1984; 21:173-85.
4. Balakumbahan R, Rajamani K, Kumanan K. Acorus calamus: An overview. Jour Med Plants Res. 2010; 4: 2740-2745.
5. Copping LG. Crop protection agents from nature. In: Natural products and analogins: The Royal Society of Chemistry, Cambridge, 1996, 501.
6. Cavazza G. Les chimiotypes parmi les plantes aromatiques cas du calamus polyploide. Ann Fals Exp Chins, 1976; 69:833-844.
7. Archana Parki, Pinky Chaubey, Om Prakash, Ravendra Kumar and Anil K. Pant. Seasonal Variation in Essential Oil Compositions and Antioxidant Properties of Acorus calamus L. Accessions. Medicines, 2017; 4:81. https://doi.org/10.3390/medicines4040081 PMid:29117116 PMCid:PMC5750605
8. Kaufman PB, Cseke LJ, Warber S, Duke JA, Brielmann, Natural products from plants, CRC press, New York, 1999, pp. 20-22. https://doi.org/10.1201/9780849331343
9. Braca A, Tommasi ND, Bari LD, Pizza C, Politi M, Morelli I, J Nat Prod, 2001; 64:892-895. https://doi.org/10.1021/np0100845 PMid:11473417
10. Oyaizu M. Studies on products of browning reactions: antioxidant activities of products of browning reaction prepared from glucosamine. J Nutrit; 1986; 44:307-15. https://doi.org/10.5264/eiyogakuzashi.44.307
11. Prieto P, Pineda M, Aguilar M. Spectrophotometric Quantitation of Antioxidant Capacity through the Formation of a Phosphomolybdenum Complex: Specific Application to the Determination of Vitamin E1. Analytical Biochem, 1999; 269:337- 341 https://doi.org/10.1006/abio.1999.4019 PMid:10222007
12. Nanda B L, Sayeeda Nigar Sultana G and Thulasi T Radhakrishnan.Determination of phytochemicals and antioxidant activity of acorus calamus rhizome. Journal of Drug Delivery & Therapeutics; 2014; 4(6):117-121 https://doi.org/10.22270/jddt.v4i6.1005
13. Amit Bahukhandi, Sandeep Rawat, Indra D. Bhatt, Ranbeer S. Rawal . Influence of Solvent Types and Source of Collection on Total Phenolic Content and Antioxidant Activities of Acorus calamus L. Natl. Acad. Sci. Lett, January-February 2013; 36(1):93-99. https://doi.org/10.1007/s40009-012-0109-8
14. G. S. Suhartati Djarkasi, Lana E. Lalujan, Erny Nurali and Maria F. Antioxidant Activity of Karimenga (Acorus calamus). AIP Conference Proceedings 2019; 2155:020051. https://doi.org/10.1063/1.5125555
15. G.C. Bag, P. Grihanjali Devi, Th. Bhaigyabati. Phytochemical Screening and Antioxidant Activity of Meyna laxiflora Species Found in Imphal West District of Manipur. Int. J. Pharm. Sci. Rev. Res., January - February 2016, 36(1):137-143
16. Olayinka A, Aiyegoro AI, Okoh. Preliminary phytochemical screening and in vitro antioxidant activities of the aqueous extract of Helichrysum longifolium DC, BMC Complementary and Alternative Medicine, Oxford University Press, 2010; 10:21. https://doi.org/10.1186/1472-6882-10-21 PMid:20470421 PMCid:PMC2877649
17. Oktay M, Gulcin I, Kufrevioglu OI. Determination of in vitro antioxidant activity of fennel (Foeniculum vulgare) seed extracts, Lebensum. Wiss. U. Technol, 2003; 36:263-271. https://doi.org/10.1016/S0023-6438(02)00226-8
18. Chanda S, Dave R . In vitro models for antioxidant activity evaluation and some medicinal plants possessing antioxidant properties: An overview, Afr J Microbiol Res., 2009; 3(13):981-996.
Crossmark
Statistics
121 Views | 46 Downloads
How to Cite
1.
Sharma L, Khan M, Devi A. Methanolic and ethanolic phytochemical screening of Sweet flag (Acorus calamus L.) rhizome. JDDT [Internet]. 15Sep.2023 [cited 2Oct.2023];13(9):114-8. Available from: https://jddtonline.info/index.php/jddt/article/view/6223
Section
Research
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0). that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
.