Evaluation of the effects of Momordica charantia on tibial defect injury in rats
Abstract
We aimed to examine the histological and immunohistochemical effects of Momordica charantia on bone repair, which provides positive regulation such as blood sugar, blood pressure, antilipidemic, anticarcinogenic, antibacterial, antioxidant, anti-inflammatory, wound healing, and tissue regeneration in rats with tibial bone defect. Momordica charantia (MC) (commonly called bitter melon; Goya; bitter apple; bitter gourd; bitter squash; balsam-pear; with many more names listed below) is a tropical and subtropical vine of the family Cucurbitaceae, widely grown in Asia, Africa, and the Caribbean for its edible fruit. Its many varieties differ substantially in the shape and bitterness of the fruit. In this study, 32 male Sprague Dawley rats, 12 weeks old and weighing 250-300gr, were used. 8 rats in each group randomly, 1st group control, 2nd group defect (Sham), 3rd group defect + MC group (14 days), 4th group defect + MC group (28 days) separated into the group. In this experimental study, 600 mg/kg/day MC (bitter melon) extract was mixed into the drinking water of groups 3 and 4 and administered to rats by oral tube. One 6 mm diameter cylindrical defects were created in the body of the right tibia bone. No action was taken in Group 1. In Group 2, only tibial defect was made. For 14 days in group 3 and 28 days in group 4, 600 mg/kg/day MC extract was mixed with drinking water and given by oral gavage. Elevated Malondialdehyde (MDA) levels and Myeloperoxidase (MPO) decreased. In groups 3 and 4, osteoblastic activity and osteocyte development increased, while osteopontin and osteonectin expression were found to be positive, while osteoclastic activity decreased compared to the sham group. It is a good antioxidant in the groups where MC is applied. It has been observed that it can have a positive effect on bone repair.
Keywords: Momordica charantia; Tibial defect; Fracture healing; Bone repair; Antioxidant
Keywords:
Momordica charantia, Tibial defect, Fracture healing, Bone repair, AntioxidantDOI
https://doi.org/10.22270/jddt.v13i6.6107References
Baloğlu M, Özkorkmaz EG. Biochemical and immunohistochemical investigations on bone formation and remodelling in ovariectomised rats with tamoxifen citrate administration. Folia Morphol (Warsz)., 2019; 78:789-797, https://doi.org/10.5603/FM.a2019.0035
Kelm RJ Jr, Swords NA, Orfeo T, Mann KG. Osteonectin in matrix remodeling. A plasminogen-osteonectin-collagen complex. J Biol Chem., 1994; 269:30147-53. https://doi.org/10.1016/S0021-9258(18)43789-1
Walters G, Pountos I, Giannoudis PV. The cytokines and micro-environment of fracture haematoma: Current evidence. J Tissue Eng Regen Med., 2018; 12:e1662-e1677. https://doi.org/10.1002/term.2593
Kuroda S, Virdi AS, Dai Y, Shott S, Sumner DR. Patterns and localization of gene expression during intramembranous bone regeneration in the rat femoral marrow ablation model. Calcif Tissue Int., 2005; 77:212-25. https://doi.org/10.1007/s00223-004-0267-x
Grover JK, Yadav SP. Pharmacological actions and potential uses of Momordica charantia: a review. J Ethnopharmacol., 2004; 93:123-32, https://doi.org/10.1016/j.jep.2004.03.035
Jia S, Shen M, Zhang F, Xie J. Recent Advances in Momordica charantia: Functional Components and Biological Activities. Int J Mol Sci., 2017; 18: 2555. https://doi.org/10.3390/ijms18122555
Basch E, Gabardi S, Ulbricht C. Bitter melon (Momordica charantia): a review of efficacy and safety. Am J Health Syst Pharm., 2003; 60:356-9, https://doi.org/10.1093/ajhp/60.4.356
Taş F, Erdemci F, Aşır F, Maraşlı M, Deveci E. Histopathological examination of the placenta after delivery in pregnant women with COVID-19. J Health Sci Med, 2022; 5(3):868-874. https://doi.org/10.32322/jhsm.1100731
Peker N, Deger U, Asır F. Investigation of the effects of carvacrol on experimental ischemia/reperfusion model of rat ovaries by immunohistochemistry. Anal Quant Cytopathol Histopathol, 2021; 42 (6):197-204
Kumar M, Rawat S, Nagar B, Kumar A, Pala NA, Bhat JA, Bussmann RW, Cabral-Pinto M, Kunwar R. Implementation of the Use of Ethnomedicinal Plants for Curing Diseases in the Indian Himalayas and Its Role in Sustainability of Livelihoods and Socioeconomic Development. Int J Environ Res Public Health., 2021; 18:1509. https://doi.org/10.3390/ijerph18041509
Ekor M. The growing use of herbal medicines: issues relating to adverse reactions and challenges in monitoring safety. Front Pharmacol., 2014; 4:177. https://doi.org/10.3389/fphar.2013.00177
Doosti-Moghaddam M, Miri HR, Ghahghaei A, Hajinezhad MR, Saboori H. Effect of unripe fruit extract of Momordica charantia on total cholesterol, total triglyceride and blood lipoproteins in the blood of rats with hyperlipidemia. Cell. Mol. Biomed. Rep., 2022, 2:74-86. https://doi.org/10.55705/cmbr.2022.338806.1038
Yang YS, Wu NY, Kornelius E, Huang CN, Yang NC. A randomized, double-blind, placebo-controlled trial to evaluate the hypoglycemic efficacy of the mcIRBP-19-containing Momordica charantia L. fruit extracts in the type 2 diabetic subjects. Food Nutr Res., 2022; 66. https://doi.org/10.29219/fnr.v66.3685
Kayal RA, Tsatsas D, Bauer MA, Allen B, Al-Sebaei MO, Kakar S, Leone CW, Morgan EF, Gerstenfeld LC, Einhorn TA, Graves DT. Diminished bone formation during diabetic fracture healing is related to the premature resorption of cartilage associated with increased osteoclast activity. J Bone Miner Res., 2007; 22: 560-8. https://doi.org/10.1359/jbmr.070115
Zhang T, Han W, Zhao K, Yang W, Lu X, Jia Y, Qin A, Qian Y. Psoralen accelerates bone fracture healing by activating both osteoclasts and osteoblasts. FASEB J., 2019; 33:5399-5410. https://doi.org/10.1096/fj.201801797R
Ko EA, Park YJ, Yoon DS, Lee KM, Kim J, Jung S, Lee JW, Park KH. Drug repositioning of polaprezinc for bone fracture healing. Commun Biol., 2022; 5:462. https://doi.org/10.1038/s42003-022-03424-7
Zheng S, Zhou C, Yang H, Li J, Feng Z, Liao L, Li Y. Melatonin Accelerates Osteoporotic Bone Defect Repair by Promoting Osteogenesis-Angiogenesis Coupling. Front Endocrinol (Lausanne)., 2022; 13:826660. https://doi.org/10.3389/fendo.2022.826660
Haznedar B, Deveci E, Gök, E. Effect of Mancozeb Inhalation on the Olfactory Mucosa in Rats. International Journal of Otolaryngology and Head & Neck Surgery, 2022; 11(5):227-233. https://doi.org/10.4236/ijohns.2022.115024
Haznedar B, Ertugrul G, Deveci, E, Haznerdar B, Gök E, Deveci E. Effect of Cadmium Chloride Toxicity on Olfactory Mucosa. Int. J. Morphol, 2022; 1(2):3. https://doi.org/10.4067/S0717-95022022000601574
Golestani S, Golestaneh A, Gohari AA. Comparative effects of systemic administration of levofloxacin and cephalexin on fracture healing in rats. J Korean Assoc Oral Maxillofac Surg., 2022; 48:94-100. https://doi.org/10.5125/jkaoms.2022.48.2.94
Fattahi R, Mohebichamkhorami F, Khani MM, Soleimani M, Hosseinzadeh S. Aspirin effect on bone remodeling and skeletal regeneration. Tissue Cell., 2022; 76:101753. https://doi.org/10.1016/j.tice.2022.101753
Nordsletten L, Madsen JE, Almaas R, Rootwelt T, Halse J, Konttinen YT, Hukkanen M, Santavirta S. The neuronal regulation of fracture healing: effects of sciatic nerve resection in rat tibia. Acta Orthop Scand., 1994; 65:299-304. https://doi.org/10.3109/17453679408995457
Hao Y, Ma Y, Wang X, Jin F, Ge S. Short-term muscle atrophy caused by botulinum toxin-A local injection impairs fracture healing in the rat femur. J Orthop Res., 2012; 30:574-80. https://doi.org/10.1002/jor.21553
Published
Abstract Display: 628 
PDF Downloads: 569 PDF Downloads: 29 How to Cite
Issue
Section
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0). that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
How to Cite
.