Diuretic effects and subacute toxicity of Trema orientalis Linn leave extract in wistar rats
Abstract
Trema Orientalis is a medicinal plant used in the treatment of cardiovascular diseases including hypertension. The present study was undertaken to investigate phytochemical compounds, diuretic activity and subacute toxicity of the hydromethanolic leaf extracts of Trema orientalis. Phytochemical analysis carried out through color and precipitation reactions revealed the presence of many metabolites. The measurement of diuretic activity carried out by saline overload with 0.9% NaCl at a dose of 50 mL/kg in wistar rats showed that the hydromethanolic extract of Trema orientalis leaves had diuretic activity at a dose of 200 mg/kg of body weight. However, this diuretic activity was relatively low compared to the action of the reference molecule, furosemide. The study of the subacute toxicity of the extract took place over 28 days. No significant difference was recorded in the body weights during the 28 days of the study. Likewise, the weight of the organs collected after sacrifice of the rats and their macroscopic aspects did not show any significant difference with those of the control group. The extract increased the count of white and red blood cells as wells as platelets, practically at the two doses used and caused a decrease in ALT at the dose of 500 mg/kg. The extract could have a beneficial effect like a boosting immunity and would not present any toxicity at these doses.
Keywords: phytochemical, diuretic, subacute toxicity, Trema orientalis
Keywords:
phytochemical, diuretic, subacute toxicity, Trema orientalisDOI
https://doi.org/10.22270/jddt.v13i4.6027References
Capasso F, Gaginella TS, Grandolini G, et al. Phytotherapy. Springer Berlin Heidelberg: Berlin, Heidelberg. 2003. https://doi.org/10.1007/978-3-642-55528-2
Millogo GRC, Zongo RFE, Benao A, et al. Prévalence et caractéristiques des effets indésirables des antihypertenseurs chez les patients suivis en ambulatoire au Centre Hospitalier Universitaire Yalgado Ouédraogo. Pan Afr Med J. 2018; 29(84). https://doi.org/10.11604/pamj.2018.29.84.13754
Katende AB, Birnie A, Tengnas BO. Useful trees and shrubs for Uganda. Identif Propag Manag Agric Pastor Communities Reg Soil Conserv Unit RSCU Swed Int Dev Auth SIDA. 1995;1-710.
Ciganda C, Laborde A. Herbal Infusions Used for Induced Abortion. J Toxicol Clin Toxicol. 2003 ; 41(3):235-239. https://doi.org/10.1081/CLT-120021104
Tourneux H. Eklu-Natey Raphaël D., Balet Annie (en collab. avec M. A. Ahyi, E. J. Adjanohoun, L. Ake Assi, F. Borst, C. Chatelain, D. Diallo, K. Hostettmann, L. Sanou, M. Koumaré), 2012, Pharmacopée africaine, Dictionnaire et monographies multilingues du potentiel médicinal des plantes africaines. Afrique de l'Ouest. J Afr. 2013; (83-2):202-205. https://doi.org/10.4000/africanistes.3459
Kadissoli B, Mouzou A, Tekou E, et al. Effet de l'extrait semi-éthanolique des feuilles de Trema guineensis (Celtidacea) sur la musculature lisse du rat Wistar. Int J Biol Chem Sci. 2012; 6. https://doi.org/10.4314/ijbcs.v6i3.6
Houghton PJ, Raman A, Houghton PJ, et al. Practical exercises in chemical screening and fractionation of crude extracts from plant materials. Lab Handb Fractionation Nat Extr. 1998; 154-183. https://doi.org/10.1007/978-1-4615-5809-5_10
El-Haoud H, Boufellous M, Berrani A, et al. Screening phytochimique d'une plante medicinal: Mentha spicata L. Am J Innov Res Appl Sci. 2018; 7:226-233.
Lipschitz WL, Hadidian Z, Kerpcsar A. Bioassay of Diuretics. J Pharmacol Exp Ther. 1943; 79(2):97-110.
Assogba FM, Aderomou C, Agbodjogbe W, et al. Evaluation of diuretic propertiesfrom Elaeis guineensis Jacq. (Arecaceae) leaves aqueous extraction Wistar rat. J. Chem. Pharm. Res. 2015; 7(3):2457-2462.
Dembele O, Diallo D, Karadji Ayarga H, et al. Activité diurétique et salidiurétique d'une recette utilisée en médecine traditionnelle pour le traitement de l'hypertension artérielle. Mali Méd En Ligne. 2009;1-4.
Kau ST, Keddie JR, Andrews D. A method for screening diuretic agents in the rat. J Pharmacol Methods. 1984; 11(1):67-75. https://doi.org/10.1016/0160-5402(84)90054-8
Rhiouani H, El-Hilaly J, Israili ZH, et al. Acute and sub-chronic toxicity of an aqueous extract of the leaves of Herniaria glabra in rodents. J Ethnopharmacol. 2008; 118(3):378-386. https://doi.org/10.1016/j.jep.2008.05.009
Waynforth HB, Flecknell PA. Experimental and Surgical Technique in the Rat. Academic press London; 1980.
Guindo I. Etude du traitement traditionnel de l'hypertension artérielle au Mali. Bamako Thèse Pharm FMPOS. 2005;126.
Haïdara M. Contribution à l'étude de l'activité Pharmacologique de Terminalia Macroptera Guill. et Perr.(Combretaceae) Dans Le but de l'élaboration d'un Médicament Traditionnel Amélioré Au Mali (Afrique de l'Ouest). PhD Thesis. Université Paul Sabatier-Toulouse III. 2018.
Loumpangou CN. Caractérisation Phytochimique et toxicité aigüe des feuilles de Trema Orientalis (L.) Blume (Cannabaceae). J. Soc. Ouest-Afr. Chim. 2016; 042:52- 59.
Mera I, González Falconí DE, Morera V. Secondary Metabolites in Plants: Main Classes, Phytochemical Analysis and Pharmacological Activities. 2020. https://doi.org/10.21931/RB/2019.04.04.11
Goetz P. Y a-t-il un diurétique phytothérapique ? Phytothérapie. 2020; 18(5):301-304. https://doi.org/10.3166/phyto-2020-0236
Uddin SN, Yesmin MN, Pramanik M, et al. Anti-inflammatory, antinociceptive and diuretic activities of Trema orientalis Linn. Orient Pharm Exp Med. 2009; 9(4):320-325. https://doi.org/10.3742/OPEM.2009.9.4.320
Bentabet N, Younes I, Meftahi C. Évaluation de l'activité anti-inflammatoire et de la toxicité in vitro des extraits d'écorce de grenade Punica granatum, sur la stabilité membranaire du globule rouge. Phytothérapie. 2022 . https://doi.org/10.3166/phyto-2022-0316
Agbodjogbe W, Aïkpe JFA, Ayedoun MA, et al. Diuretic and natriuretic activities from ten medicinal plants used in south Benin. J Chem Pharm Res. 2015; 7(12):1145-52.
Kasmi S. Etude de l'activité diurétique des extraits éthanoliques de Fraxinus angustifolia et Pistacia lentiscus. 2014.
Hemalatha T, Mary DA, Ganthi AS. Acute and sub-acute toxicity study of Trema orientalis (L.) Bl. methanol extract in rats. J Drug Deliv Ther. 2019; 9(1-s):307-311. https://doi.org/10.22270/jddt.v9i1-s.2353
Muhammad AA, Ibrahim RB, Aminu C, et al. Activity of aqueous extract of Phyllanthus niruri Linn in 2, 4-dinitrophenylhydrazine induced anaemic rats. J Pharm Pharmacol Res. 2020; 4(3):79-95. https://doi.org/10.26502/fjppr.034
Chabert A, Hamzeh-Cognasse H, Cognasse F, et al. Platelets and coagulation during bacterial infections. Sang Thromb Vaiss. 2017; 29(2):61-67. https://doi.org/10.1684/stv.2017.0967
Kasim LS, Badejo MV, Daodu JO, et al. Haematopoietic and Safety Study of Methanolic Extract of the Bark of Trema orientalis (L.) Blume Fam. Ulmaceae. Niger J Pharm Appl Sci Res. 2015; 4(3):42-48.
Sangare MM, Bayala B, Ategbo J-M, et al. Effets de l'extrait aqueux de gomphrena celosioides (amaranthaceae) sur les enzymes hépatiques. Afr Sci Rev Int Sci Technol. 2012; 8(3):107-115.
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