Detection of Fungal Contamination of Ward Furnishing and Medical Equipment Used in intensive Care Unit and Neonatal Intensive Care Unit
Abstract
Background: Nosocomial infections acquired during hospitalization depend on the characteristics of the microorganisms, with a high risk of being acquired when the contaminated environment. Cross-transmission of microorganisms by contaminated surfaces and the hands of health care workers are considered to be the main route of the spread of nosocomial infections.
Aim of study: This study aim to detect the fungal contamination of ward furnishings and medical equipment used in the intensive care unit and Neonatal intensive care unit.
Materials and Methods: Fifty environmental swabs were collected from ward furnishings and medical equipment including predefined surfaces (armrest beds, wash sinks, medical tables), between August and September 2021.Swab specimens were immediately inoculated onto plates that contained Sabouraud Dextrose Agar. After that samples were incubated for a 1 to 7 days period at 28°C and checked every day for growth.
Results: Out of fifty swabs samples, 21(42%) yielded Fungal growth. From these isolated organisms Penicillum species was the predominant isolate 10 (47.6%) followed byAspergillus flavus.Penicillum species was the commonest fungal isolates among ICU, while Aspergillus flavus were the predominant isolates among Neonatal ICU. The most contaminant Equipment was found in Neonatal ICU samples 5(55.6%), and the most contaminant Place in ICU was the floors 3(25%).
Conclusion: This study showed that ICU of the hospital may contain fungi indicating that may form potential source of cross-infection through health care workers to their patients.
Keywords: Fungi, Neonatal, ICU, and Infection.
Downloads
References
2-Amend AS, Seifert KA, Samson R, Bruns TD. Indoor fungal composition is geographically patterned and more diverse in temperate zones than in the tropics. ProcNatlAcadSci USA 2010; 107:13748-13753 https://doi.org/10.1073/pnas.1000454107
3-Singh J: New advances in identification of fungal damage in buildings. The Mycologist, 2016; 1991: 5(3):139-140 https://doi.org/10.1016/S0269-915X(09)80308-X
4-Sandle, T. Risk Consideration for Aging Pharmaceutical Facilities, Journal of Validation Technology, 22(2):11-20
5-Tang W, Kuehn T. H. , Simcik MF. Effects of Temperature, Humidity and Air Flow on Fungal Growth Rate on Loaded Ventilation Filters, Journal of Occupational and Environmental Hygiene, 2015; 12:8:525-537 https://doi.org/10.1080/15459624.2015.1019076
6-Findley, K., Oh, J., Yang, J., Conlan, S. et al. Topographic diversity of fungal and bacterial communities in human skin, Nature 2013; 498:367-370 https://doi.org/10.1038/nature12171
7-Hayes, B. "Managing Aseptic Gowning with in Classified Environments." Cleanroom Technology. 2015.: http://www.cleanroomtechnology.com/technical/article_page/Managing_asept...
8-Richardson, M. D. and D. W. Warnock, Fungal Infection: Diagnosis and managementFourth Edition Wiley-Blackwell. 2012; pp445 https://doi.org/10.1002/9781118321492
9-Dong J., M.J. Loeffelholz and M. R. McGinnis. Sequence-based fungal identification and classification. In Molecular Microbiology: Diagnostic Principles and Practice Second Edition ASM Press. 2012; pp669-676 https://doi.org/10.1128/9781555816834.ch43
10-Nelsson, R. H., K. Abarenkov, K-H. Larsson and U. Koljalg, Molecular identification of fungi: rationale, philosophical concerns and the UNITE database. Open Appl. Inform. J 2011; 5(Suppl. 1-M9):81-86 https://doi.org/10.2174/1874136301105010081

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0). that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).