Synergistic Effects of Essential Oils and Antibiotics Against Some Bacterial Strains

Authors

  • Egoume Gaizirene Feudjieu Pharmacology and Drugs Discovery Laboratory, Center For Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plants Studies, P.O. Box 13033, Yaounde, Cameroon; http://orcid.org/0000-0001-7120-3647
  • Gislaine Aurelie Kemegne Pharmacology and Drugs Discovery Laboratory, Center For Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plants Studies, P.O. Box 13033, Yaounde, Cameroon;
  • Fossi Cedric Tchinda Pharmacology and Drugs Discovery Laboratory, Center For Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plants Studies, P.O. Box 13033, Yaounde, Cameroon; http://orcid.org/0000-0003-3206-0916
  • Deutou Armelle Tchamgoue Pharmacology and Drugs Discovery Laboratory, Center For Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plants Studies, P.O. Box 13033, Yaounde, Cameroon; http://orcid.org/0000-0002-3431-2059
  • Esther Del Florence Moni Ndedi Department of Microbiology, Faculty of Sciences, University of Yaounde I, P.O. Box 812, Yaounde, Cameroon; http://orcid.org/0000-0002-0693-7641
  • Gayap Sonia Matchuenkam Pharmacology and Drugs Discovery Laboratory, Center For Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plants Studies, P.O. Box 13033, Yaounde, Cameroon; http://orcid.org/0009-0005-3279-2676
  • Gabriel A. Agbor Pharmacology and Drugs Discovery Laboratory, Center For Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plants Studies, P.O. Box 13033, Yaounde, Cameroon; http://orcid.org/0000-0001-5860-9853

Abstract

The emergence of antibiotics resistances become an alarming situation due to the resurgence of cases. Scientific community explore therapeutics alternatives, including essentials oils and their combination with antibiotics. The designed study aimed to evaluate the combined effects of essentials oils from Drypetes Gosswelleri, Echinops giganteus, Melaleuca leucadendron essentials oils and antibiotics against strains implicated in infectious deseases. The antibacterial effect of essentials oils and antibiotics (Ciprofloxacine and Ceftriaxone) was carried out against Pseudomonas aeruginosa, Klebsiella pnemoneae, Salmonella enteritidis, Staphylococcus aureus and Bacillus cereus strains using the microdilution method. The synergistic effects were studied by the isobologram method to conclude on the interaction type. The results revealed that the essential oils were active against all the tested bacteria. Drypetes Gosswelleri EO showed the strongest activity with MICs ranging from 1.46 μg/mL to 11.71  µg/mL, followed by Echinops giganteus essential oil with MICs ranging from 2.92 µg/mL to 23.43 µg/mL and Melaleuca leucadendron had the lowest activity with MICs ranging from 5.88µg/mL to 750µg/mL. The combinations realized between Drypetes Gosswelleri and Echinops giganteus EOs and with two antibiotics use to evaluate the interaction type agains Salmonella enteritidis and Staphylococcus aureus. MICs obtained with the combinations are lower than those obtained with the EOs and antibiotics tested individually. The isobolograms plotted inform about a synergistic effects of combinations made with Drypetes Gosswelleri, Echinops giganteus EOs, Ciprofloxacin and Ceftriaxone against S. enteritidis and S. aureus strains. Based on the results obtained, the combinations between EOs and the antibiotocs, represent an interesting therapeutic alternative.

Keywords: antibacterial, essential oil, combination, isobolograms, synergistics, Minimale Inhibitory Concentration, Minimale Bactericidal Concentration.

Keywords:

antibacterial, essential oil, combination, isobolograms, synergistics, Minimale Inhibitory Concentration, Minimale Bactericidal Concentration

DOI

https://doi.org/10.22270/jddt.v13i6.5860

Author Biographies

Egoume Gaizirene Feudjieu, Pharmacology and Drugs Discovery Laboratory, Center For Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plants Studies, P.O. Box 13033, Yaounde, Cameroon;

Department of Microbiology, Faculty of Sciences, University of Yaounde I, P.O. Box 812, Yaounde, Cameroon;

Laboratory for Tuberculosis Research, The Biotechnology Center, University of Yaounde I, P.O. Box 812, Yaounde, Cameroon

Gislaine Aurelie Kemegne, Pharmacology and Drugs Discovery Laboratory, Center For Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plants Studies, P.O. Box 13033, Yaounde, Cameroon;

Department of Microbiology, Faculty of Sciences, University of Yaounde I, P.O. Box 812, Yaounde, Cameroon;

Fossi Cedric Tchinda, Pharmacology and Drugs Discovery Laboratory, Center For Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plants Studies, P.O. Box 13033, Yaounde, Cameroon;

Department of Microbiology, Faculty of Sciences, University of Yaounde I, P.O. Box 812, Yaounde, Cameroon;

Laboratory for Tuberculosis Research, The Biotechnology Center, University of Yaounde I, P.O. Box 812, Yaounde, Cameroon

Deutou Armelle Tchamgoue, Pharmacology and Drugs Discovery Laboratory, Center For Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plants Studies, P.O. Box 13033, Yaounde, Cameroon;

Pharmacology and Drugs Discovery Laboratory, Center For Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plants Studies, P.O. Box 13033, Yaounde, Cameroon;

Esther Del Florence Moni Ndedi, Department of Microbiology, Faculty of Sciences, University of Yaounde I, P.O. Box 812, Yaounde, Cameroon;

Laboratory for Tuberculosis Research, The Biotechnology Center, University of Yaounde I, P.O. Box 812, Yaounde, Cameroon

Gayap Sonia Matchuenkam, Pharmacology and Drugs Discovery Laboratory, Center For Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plants Studies, P.O. Box 13033, Yaounde, Cameroon;

Pharmacology and Drugs Discovery Laboratory, Center For Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plants Studies, P.O. Box 13033, Yaounde, Cameroon;

Gabriel A. Agbor, Pharmacology and Drugs Discovery Laboratory, Center For Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plants Studies, P.O. Box 13033, Yaounde, Cameroon;

Pharmacology and Drugs Discovery Laboratory, Center For Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plants Studies, P.O. Box 13033, Yaounde, Cameroon;

References

Philippon A, Résistance bactérienne : définitions, mécanismes, évolution, EMC-Maladies infectieuses, 2008; 5(3):1-13. https://doi.org/10.1016/S1166-8598(08)26016-3

Cheesman MJ, Ilanko A, Blonk B, Cock IE, Developing new antimicrobial therapies: Are synergistic combinations of plant extracts/compounds with conventional antibiotics the solution?, Pharmacognosy Reviews, 2017; 11(22):57-72. https://doi.org/10.4103/phrev.phrev_21_17

Delaquis PJ, Stanich K, Girard B, Mazza G, Antimicrobial activity of individual and mixed fractions of dill, cilantro, coriander and eucalyptus essential oils, International Journal of Food Microbiology, 2002; (74):101-109. https://doi.org/10.1016/S0168-1605(01)00734-6

Harris R, Synergism in the essential oil world, International Journal of Aromatherapy, 2003; 12:179-186. https://doi.org/10.1016/S0962-4562(02)00083-8

Jia J, Feng Z, Xiaohua M, Zhiwei WC, Yixue XL, Yu ZC, Mechanisms of drug combinations: interaction and network perspectives, Nature Reviews Drug Discovery, 2009; 8:111-128. https://doi.org/10.1038/nrd2683

Sun X, Vilar S, Tatonetti NP, High-throughput methods for combinatorial drug discovery, Science Translational Medicine, 2013; 5(205):205rv1-205rv1. https://doi.org/10.1126/scitranslmed.3006667

Baldé AM, Traoré MS, Diallo MST, Baldé ES, Huang Y, Liu Z, Oularé K, Barry MS, Baldé MA, Camara A, Berghe DV, Vlietinck A, Pieters L, Ethnobotanical survey, antimicrobial and anticomplement activities of Guinean medicinal plants traditionally used in the treatment of inflammatory diseases in Conakry and Dubreka, Journal of Plant Sciences, 2015; 3(1-2):11-19.

Wessal O, Samira B, Mounyr B, Abdelhakim EOL, Sandrine M, Fouad OC, Hassane G, Chemical composition of Citrus aurantium L. leaves and zest essential oils, their antioxidant, antibacterial single and combined effects, Journal of Chemical and Pharmaceutical Research, 2015 ; 7(1):78-84.

Franchomme P, l'aromatherapie à visée anti infectieuse, Phytomedecine, 1981; 1(2):25-47

Hala GM, Christ H, Carla K, Traditional uses of Salvia libanotica (East Mediterranean sage) and the effects of essential oils, Journal of Ethnopharmacology, 2000; 71(3):513-520. https://doi.org/10.1016/S0378-8741(99)00190-7

Caillet S, et Lacroix M, Les huiles essentielles : leurs propriétés antimicrobiennes et leurs applications potentielles en alimentaire, INRS-Institut Armand-Frappier, (RESALA), Canada, 2007; P :1- 8.

Siddiqui YM, Ettayebi M, Haddad AM, Al-Ahdal MN, Effect of essential oils on the enveloped viruses : antiviral activity of oregano and clove oils on herpes simplex virus type 1 and Nawcastle disease virus, Medical science Research, 1996; 24:185-186.

Roux D. Conseil en aromathérapie. France. 2e Ed. pro-Officina : Wolters Kluwer. 2008. P. 261-267.

Rhayour K, Bouchiki T, Tantaoui-Elaraki A, Sendide K, Remmal A, The mechanism of bactericidal action of oregano and clove essential oil and their phenolic major components on Escherichia coli and Bacillus cereus, Journal of Essential oil Research, 2003; 15(5):356-362. https://doi.org/10.1080/10412905.2003.9698611

Bruneton J. Huiles essentielles, dans Pharmacologie: phytochimie, plantes médicinales. 2e ed. Lavoisier : Paris ; 1993. P.406-466.

Oussou KR, Yolou SF, Tue BB, Kanko C, Boti JB, Ahibo C, Etude Chimique Bio-Guidée de l'huile Essentielle de Ocimum Gratissimum, Journal casanova, 2010; 40(1):50-59.

Khaddar MF, Alternatives à l'antibiothérapie par les huiles essentielles et synergie d'action. Sciences pharmaceutiques, dumas-01840585, 2018.

Kemegne AG, Sado KSL, Nyegue MA, Menut C And Etoa FX, Comparing checkerboard, isobologram and CCD methods for drug combination: A case study of ciprofloxacin and plant extracts on Escherichia coli and Shigella, Journal of Medicinal Plants Research, 2021; 15(10):479-489.

Tchinda AT, Sob VST, Drypetes gossweileri S.Moore. In : Schmelzer, G.H. and Gurib-Fakim, A. (Editeurs). Prota Medicinal plants/Plantes médicinales, 2008; 11(1)

Menut C, Lamaty G, Weyerstahl P, Marschall H, Seelmann I, et Amvam

ZPH, Aromatic plants of tropical Central Africa. Part XXXI. Tricyclic sesquiterpenes from the root essential oil of Echinops giganteus var. lelyi C. D. Adams, Flavour Fragrance Journal, 1997; 12:415-421. https://doi.org/10.1002/(SICI)1099-1026(199711/12)12:6<415::AID-FFJ666>3.0.CO;2-T

Pujiarti R, Ohtani Y, Widowati, T.B, Kasmudjo Utilization of Melaleuca leucadendron essential oil, Indonesian Journal of Wood Science, 2012; (2):94-99.

Collins LA, Franzblau SG. Microplate Alamar Blue Assay versus BACTEC 460 system for high-throughput screening of compounds against Mycobacterium tuberculosis and Mycobacterium avium, Antimicrobial Agents Chemotherapy, 199; 41:1004-1009. https://doi.org/10.1128/AAC.41.5.1004

Jimenez A, Meckes A, Ramirez M, Torres R, Luna-Herrera J, Activity against multidrug-resistant Mycobacterium tuberculosis in Mexican plants used to treat respiratory diseases, Phototherapy Research, 2003; 17:903-908. https://doi.org/10.1002/ptr.1377

CLSI (Clinical and Laboratory Standards Institute). Susceptibility testing of Mycobacteria, Nocardiae and other aerobic actinomycetes. Approved-second edition M24-A2 Wayne, 2011.

Fauchère JL, Avril JL. Bacteriologie générale et médicale. Ed. Elipses :rue Bargues 75740 Paris cedex 15; 2002. P.165.

Fivelman QL, Adagu IS, Warhurst DC, Modified fixed-ratio isobologram method for studying in vitro interactions between atovaquone and proguanil or dihydroartemisinin against drug-resistant strains of Plasmodium falciparum, Antimicrobial Agents Chemotherapy, 2004; 48(11):4097-4102. https://doi.org/10.1128/AAC.48.11.4097-4102.2004

Wessal O, Samira B, Mounyr B, Abdelhakim EOL, Sandrine M, Fouad OC, Hassane G, Chemical composition of Citrus aurantium L. leaves and zest essential oils, their antioxidant, antibacterial single and combined effects, Journal of Chemical and Pharmaceutical Research, 2015; 7(1):78-84.

Hall MJ, Middleton RF, Westmacott D, The fractional inhibitory concentration (FIC) index as a measure of synergy, Journal of Antimicrobial Chemotherap, 1983; 11:427-433. https://doi.org/10.1093/jac/11.5.427

Krogstad DJ, Moellering RC. Antimicrobial Combinations. Antibiotics in Laboratory Medicine. 2nd ed. Lorian V, Ed, Williams & Wilkins: Baltimore, MD, USA. 1986. P. 537-595

Bickii J, Njikam N, Ayafor FI, Ringwald P, in vitro antimalarial activity of limonoids from Khaya grandifoliola C.D.C (Meliaceae), Journal of Ethnopharmacology, 2000; 69(1) : 27-33. https://doi.org/10.1016/S0378-8741(99)00117-8

Ratnakar P, Murthy S, Preliminary studies on the antitubercular activity and the mechanism of action of the water extract of garlic (Allium sativum) and its two partially purified proteins (garlic defensis), Indian Journal Clinical and Biochemistry, 1996; 11(1):37-41. https://doi.org/10.1007/BF02868409

Dorman HJD et Deans SG, Antimicrobial agents from plants: antibacterial activity of plant volatile oils, Journal of Applied Microbiology, 2000; 88 (2):308-316. https://doi.org/10.1046/j.1365-2672.2000.00969.x

Vardar-Ünlü G, Candan F, Sökmen A, Daferera D, Polissiou M, Sökmen M, Tepe B, Antimicrobial and antioxidant activity of the essential oil and methanol extracts of Thymus pectinatus Fisch. et Mey. Var. pectinatus (Lamiaceae), Journal of Agricultural and Food Chemistry, 2003; 51(1):63-67. https://doi.org/10.1021/jf025753e

Moni NDEF, Assam AJP, Nyegue MA, Feudjieu EG, Penlap BV, Etoa F-X, Anti-mycobacterial efficacy of three essentials oils from medicinal plants currently used traditionally to treat tuberculosis in Cameroon, American Journal of Essential Oil and Natural Products, 2018; 6:10-18.

Moni NEDF, Nyegue MA, Assam Assam JP, Betote DPH, Feudjieu EG, Penlap BV, and Etoa F-X : Effects of Essential oil from Drypetes gossweileri S. Moore stem barks on Cell Release and DNA Synthesis of Mycobacterium tuberculosis, Journal of Drug Delivery & Therapeutics. 2019; 9(2):319-324.

Tchinang F, Ndoye FFM, Keumoe R, Zeuko'o ME, Fekam BF and Etoa FX, In vitro anti yeast activity, kinetics and mechanism of action of essential oils from two cameroonian medicinal plants, BMC Complementary Medicine and Therapies, 2023; 23:115. https://doi.org/10.1186/s12906-022-03827-3

Eyélé MC, Menut C, Bessière JM, Lamaty G, Nzé Ekekang L, Denamganai J, Aromatic Plants of Tropical Central Africa: Benzyl isothiocyanate as major constituent of bark essential oil of Drypetes gossweileri S. Moore, Journal of Essential Oil Research, 1997; (9):367-370. https://doi.org/10.1080/10412905.1997.10554264

Ndoye F, Tchinang TF, Nyegue AM, Abdou JP, Yaya AJ, Tchinda AT, Essame JO, Etoa F-X, Composition chimique, propriétés antioxydantes et anti-inflammatoires in vitro des huiles essentielles de quatre plantes diététiques et médicinales du Cameroun, Bio Medical Central Complementary and Alternative Medecine, 2016; 16(1) 117. https://doi.org/10.1186/s12906-016-1096-y

D'Antuono LF, Elementi S, Neri R, Exploring new potential healthpromoting vegetables: glucosinolates and sensory attributes of rocket salads and related Diplotaxis and Eruca species, Journal of the Science of Food and Agriculture, 2009; (89):713-722. https://doi.org/10.1002/jsfa.3507

Hong E, Kim GH, Anticancer and antimicrobial activities of b-phenylethyl isothiocyanate in Brassica rapa L, Food Science and Technology Research, 2008 ; 14:377-382. https://doi.org/10.3136/fstr.14.377

Saavedra M.J, Borges A, Dias C, Aires A, Bennett RN, Rosa ES, Simões M, Antimicrobial activity of phenolics and glucosinolate hydrolysis products and their synergy with streptomycin against pathogenic bacteria, Medicinal Chemistry, 2010 ; 6:174-183. https://doi.org/10.2174/1573406411006030174

Kaiser ST, Mutters NT, Blessing B, Günther G, Natural isothiocyanates express antimicrobial activity against developing and mature biofilms of Pseudomonas aeruginosa, Fitoterapia, 2017; 119:57-63 https://doi.org/10.1016/j.fitote.2017.04.006

Tekwu EM, Askun Tulin, Kueté V, Nkengfack AE, Nyasse B, Etoa F-X, Penlap BV, Antibacterial activity of selected Cameroonian dietary spices ethno medically used against strains of Mycobacterium tuberculosis. Journal of Ethnopharmacology, 2012; 142: 374-382. https://doi.org/10.1016/j.jep.2012.05.003

Pavela R, Filippo M, Mbuntcha H, Woguem V, Dongmo FHP, Womeni HM, Azefack TL, Barboni L, Nicoletti M, Canale A, Benelli G, Traditional herbal remedies and dietary spices from Cameroon as novel sources of larvicides against filariasis mosquitoes, Parasitology Research, 2016; (115):4617-4626. https://doi.org/10.1007/s00436-016-5254-4

Monzote L, Scherbakov AM, Prabodh SRS, Cos P, Shchekotikhin AE, Lars G, Setzer WN, Essential oil from memaleuca leucadendra : antimicrobial, antikinetoplastid, antiproliferative and cytotoxic assessment, Molecule, 2020; 25(23):5514. https://doi.org/10.3390/molecules25235514

Rajendra CP, Ram SV, Amit C, Chandan SC, The essential oil composition of Melaleuca leucadendra grown in India: A novel source of (E)-nerolidol, Industrial Crops and Products, 2015; 69:224-227. https://doi.org/10.1016/j.indcrop.2015.02.019

Xie C, Sun L, Meng L, Wang M, Xu J, Bartlam M, Guo Y, Sesquiterpenes from Carpesium macrocephalum Inhibits Candida albicans Biofilm Formation and Dimorphism, Bioorganic & Medicinal Chemistry Letters, 2015; 25(22):5409-11. https://doi.org/10.1016/j.bmcl.2015.09.013

Rashmi S, Sujata S, Dipesh, correlation of minimum inhibitory concentration of ciprofloxacin to the therapeutic response of patient with urinary tract infection caused by escherichia coli , international journal of pharmaceutical science and research, 2014; 5(3):970-976.

Poojary A, Kumar BA, Kokare A, Pereira J, Rhora S, minimum inhibitory (mic) concentration of ceftriaxone for blood culture isolated of nalidixic acid resistant salmonella (NARS) _A 9 years study., 2020; 101(1):55-56. https://doi.org/10.1016/j.ijid.2020.09.177

Lister PD, Wolter DJ, Hanson ND, Antibacterial-resistant Pseudomonas aeruginosa: clinical impact and complex regulation of chromosomally encoded resistance mechanisms, Clinical Microbiology Review, 2009; 22:582-610 https://doi.org/10.1128/CMR.00040-09

Fadli M, Bolla JM, Mezriouti N-E, Pagès JM, Hassani L, first evidence of antibacterial and synergistic effects of Thymus riatarum essential oil with conventional antibiotics, Industrial Crops and Products, 2014; 60:370-376. https://doi.org/10.1016/j.indcrop.2014.07.029

Mayaud L, Carricajo A, Zhiri A, Aubert G, Comparison of bacteriostatic and bactericidal activity of 13 essential oils against strains with varying sensitivity to antibiotics, Letters in applied microbiology, 2008; 47(3):167-173. https://doi.org/10.1111/j.1472-765X.2008.02406.x

Malik T, Singh P, Pant S, Chauhan N, Lohani H. : Potentiation of Antimicrobial Activity of Ciprofloxacin by Pelargonium graveolens Essential Oil against Selected Uropathogens: synergism between p. graveolens essential oil and ciprofloxacin. Phytotherapy Research. 2011; 25(8):1225 8. https://doi.org/10.1002/ptr.3479

Dorman HJD et Deans SG, Antimicrobial agents from plants: antibacterial activity of plant volatile oils, Journal of Applied Microbiology, 2000; 88 (2):308-316. https://doi.org/10.1046/j.1365-2672.2000.00969.x

Bakhtia M, Fouad B, Mohamed K, Bouharaoua B, Fatiha A, evaluation of chemical composition, anti inflammatory, antibacterial activity and synergistic effect between antibiotics and the essential oil of artemisia campestris, Journal of Applied Biological Science, 2021; 16(2): 230-247.

Bassolé IHN, and Rodolfo JH, Essential Oils in Combination and Their Antimicrobial Properties, Molecules, 2012; 17: 3989-4006. https://doi.org/10.3390/molecules17043989

Rosato A, Piarulli M, Corbo F, Muraglia M, Carone A, Vitali ME, Vitali C, In vitro synergistic action of certain combinations of gentamicin and essential oils, Current medicinal chemistry, 2010; 17(28):3289-3295. https://doi.org/10.2174/092986710792231996

Malik T, Singh P, Pant S, Chauhan N, Lohani H. : Potentiation of Antimicrobial Activity of Ciprofloxacin by Pelargonium graveolens Essential Oil against Selected Uropathogens: synergism between p. graveolens essential oil and ciprofloxacin. Phytotherapy Research. 2011; 25(8):1225 8. https://doi.org/10.1002/ptr.3479

Mahmoud AM, El-Baky RMA, Ahmed ABF, Gad GFM, Antibacterial Activity of Essential Oils and in Combination with Some Standard Antimicrobials against Different Pathogens Isolated from Some Clinical Specimens, American Journal of Microbiological Research, 2016; 4(1):16 25.

Rodrigues FFG, Costa JGM, Coutinho HDM, Synergy effects of the antibiotics gentamicin and the essential oil of Croton zehntneri, Phytomedicine, 2009; 16(11):1052-1055. https://doi.org/10.1016/j.phymed.2009.04.004

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2023-06-15
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How to Cite

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Feudjieu EG, Kemegne GA, Tchinda FC, Tchamgoue DA, Moni Ndedi EDF, Matchuenkam GS, et al. Synergistic Effects of Essential Oils and Antibiotics Against Some Bacterial Strains. J. Drug Delivery Ther. [Internet]. 2023 Jun. 15 [cited 2026 Jan. 27];13(6):73-82. Available from: https://jddtonline.info/index.php/jddt/article/view/5860

Issue

Vol. 13 No. 6 (2023): Volume 13, Issue 6, June 2023

Section

Research

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How to Cite

1.
Feudjieu EG, Kemegne GA, Tchinda FC, Tchamgoue DA, Moni Ndedi EDF, Matchuenkam GS, et al. Synergistic Effects of Essential Oils and Antibiotics Against Some Bacterial Strains. J. Drug Delivery Ther. [Internet]. 2023 Jun. 15 [cited 2026 Jan. 27];13(6):73-82. Available from: https://jddtonline.info/index.php/jddt/article/view/5860
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