Molecular Characterisation of Multidrug Resistant Pathogen Isolated from Egg and its Control Measures
Abstract
Multidrug resistance is a condition enabling a disease causing microorganism to resist distinct drugs or chemical of wide variety of structure and function targeted at eradicating the bacteria. Microorganisms that display multidrug resistant can be pathogenic cells. In this study, table eggs are used commonly, because it considered the most nutritious economical source of protein that can be a part of healthy diet. However, egg shell carries bacteria which cause illness; even unbroken clean fresh shell egg may also contain harmful bacteria. In egg shell presence of Escherichia coli and Staphylococcus aureus were found. Out of total examined sample, no Salmonella was detected. Several disease occurred in poultry are caused by Staphylococcus sp. such as Staphylococcus aureus. It produces Enterotoxins which create food poisoning in consumers. Eggs are the potential source of transmitting antibiotics. Resistant Staphylococcus strain to human causing food-borne infection through Staphylococcus strains can potentially be harmful to humans. Methicillin-resistant Staphylococcus aureus (MRSA) is considered as one of the important bacterium. As a result, Multidrug resistance (MDR) in the bacteria may develop. Nowadays poultry farm increase day by day in Tamilnadu, there is a chance of transmitting the MRSA to Human through egg consumption. This present study was conducted to isolate the bacterial species and the characterization of Staphylococcus aureus along with remedial measure on egg Shell surface.
Keywords: Multidrug resistant, Pathogenic, Egg shell, Antibiotics, Human welfare
Keywords:
Multidrug resistant, Pathogenic, Egg shell, Antibiotics, Human welfareDOI
https://doi.org/10.22270/jddt.v13i6.5852References
Abulreesh, HH. And Organji, SR.The Prevalence of Multidrug-resistant Staphylococci in Food and the Environment of Makkah, Saudi Arabia. Res. J. Microbiol. 2011; 6: 510-523. https://doi.org/10.3923/jm.2011.510.523
Salihu MD, Garba B, Isah Y. Evaluation of microbial contents of table eggs at retail outlets in Sokoto metropolis, Nigeria. Sokoto J Vet Sci. 2015; 13(1):22-8. https://doi.org/10.4314/sokjvs.v13i1.4
Eid S, Nasef SA, ErfanAM. Multidrug resistant bacterial pathogens in eggs collected from backyard chickens. Assiut Vet Med J. 2015; 61(144):87-10. https://doi.org/10.21608/avmj.2015.170025
Cantón R., Morosini M.I. Emergence and spread of antibiotic resistance following exposure to antibiotics. FEMS Microbiol. Rev. 2011; 35:977-991. https://doi.org/10.1111/j.1574-6976.2011.00295.x
Sanders E. R. Aseptic laboratory techniques: plating methods. Journal of visualized experiments: JoVE.2012; 63: e3064. https://doi.org/10.3791/3064-v
O'Regan, E.; McCabe, E.; Burgess, C.; McGuinness, S.; Barry, T.; Duffy, G. Development of a real-time multiplex PCR assay for the detection of multiple Salmonella serotypes in chicken samples. BMC Microbiol. 2008; 8:156. https://doi.org/10.1186/1471-2180-8-156
Smith, S.I.; Seriki, A.; Ajayi, A. Typhoidal and non-typhoidal Salmonella infections in Africa. Eur. J. Clin. Microbiol. Infect. Dis. 2016; 35:1913-1922. https://doi.org/10.1007/s10096-016-2760-3
Meng, X.; Zhang, Z.; Li, K.; Wang, Y.; Xia, X.; Wang, X.; Xi, M.; Meng, J.; Cui, S.; Yang, B. Antibiotic Susceptibility and Molecular Screening of Class I Integron in Salmonella isolates recovered from retail raw chicken carcasses in China. Microbial. Drug Resist. 2017; 23:230-235. https://doi.org/10.1089/mdr.2015.0359
Islam, M.J.; Mahbub-E-Elahi, A.T.M.; Ahmed, T.; Hasan, M.K. Isolation and identification of Salmonella spp. from broiler and their antibiogram study in Sylhet, Bangladesh. J. Appl. Biol. Biotechnol. 2016; 4:046-051.
Kabir, M. S., &Tasmim, T. Isolation of pectinase producing bacteria from the rhizosphere of Andrographis paniculata Nees and 16S rRNA gene sequence comparison of some potential strains. Advances in Microbiology, 2019; 9(01):1. https://doi.org/10.4236/aim.2019.91001
Mishra, U. S., Mishra, A., Kumari, R., Murthy, P. N., & Naik, B. S.. Antibacterial activity of ethanol extract of Andrographis paniculata. Indian Journal of Pharmaceutical Sciences. 2009; 71(4):436. https://doi.org/10.4103/0250-474X.57294
Suriyo T, Pholphana N, Ungtrakul T, et al. Clinical Parameters following Multiple Oral Dose Administration of a Standardized Andrographis paniculata Capsule in Healthy Thai Subjects.Planta Med. 2017; 83(9):778-789. https://doi.org/10.1055/s-0043-104382
Worakunphanich W, Thavorncharoensap M, Youngkong S, et al. Safety of Andrographis paniculata: A systematic review and meta-analysis.Pharmacoepidemiol Drug Saf. 2021; 30(6):727-739. https://doi.org/10.1002/pds.5190
Saroj Kumar Sah, UbaidRasool, and S. Hemalatha. Andrographis paniculata extract inhibit growth, biofilm formation in multidrug resistant strains of Klebsiellapneumonia Complement Med. 2020; 10(6):599-604. https://doi.org/10.1016/j.jtcme.2019.02.006
Costa, M.C.; Bessegatto, J.A.; Alfieri, A.A.; Weese, J.S.; João Filho, A.B.; Oba, A. Different antibiotic growth promoters induce specific changes in the cecal microbiota membership of broiler chicken. PLoS ONE. 2017; 12:e0171642. https://doi.org/10.1371/journal.pone.0171642
Ingle KP, Deshmukh AG, Padole DA, Dudhare MS, Moharil MP, Khelurkar VC. Phytochemicals: Extraction methods, identification and detection of bioactive compounds from plant extracts. J Pharmacogn Phytochem. 2017; 6:32-6.
Published
Abstract Display: 358 
PDF Downloads: 500 PDF Downloads: 30 How to Cite
Issue
Section
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0). that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
How to Cite
.