Nanoparticles of Cerium Dioxide and Pristine (Unmodified) Fullerene C60 Protect Living Cells against Adverse Environmental Exposure: Does it Work?
Abstract
We believe that unmodified hydrated C60 fullerene (C60FWS) and nanocrystalline cerium dioxide (NCD) are promising for biological research. It is knowns that these nanoparticles protect living cells from damaging environmental factors such as radioactive and ultraviolet radiation, temperature, hypoxia, toxic substances, etc. However, little attention has been paid in scientific reports to the use and study of these nanoparticles as possible adaptogens. In our studies, we tried to protect the culture of cyanobacteria Spirulina platensis with C60 and NCD from adverse conditions. The choice of Spirulina platensis is due to its sensitivity to adverse environmental factors. The results obtained show that the absence of toxic effects of C60 and NCD of selected concentrations on the Spirulina platensis culture has been found. Nanoparticles C60 and NCD maintained the culture of Spirulina platensis for at least 4 weeks in the absence of nutrients, light, low pH storage conditions and low temperature. Cyanobacteria stored for 1–4 weeks in distilled water with nanoparticles showed increased proliferative activity compared to samples stored at the same time in the standard Zarrouk’s nutrient medium.
Keywords: fullerene C60, nanocrystalline cerium dioxide, Spirulina platensis, adaptogens.
Keywords:
fullerene C60, nanocrystalline cerium dioxide, Spirulina platensis, adaptogensDOI
https://doi.org/10.22270/jddt.v12i5-S.5697References
Eswaran SV. Water soluble nanocarbon materials: a panacea for all? Curr Sci. 2018; 114:1846-50 https://doi.org/10.18520/cs/v114/i09/1846-1850
Matyshevska OP, Prylutska SV, Grynyuk II. Fullerenes C60 - biologically active molecules І. Physicochemical properties and bioavailability. Biotechnologia Acta. 2010; 3(1):18-26.
Goltsev A, Bondarovych M, Babenko N, GaevskaYu, Dubrava T, Ostankov M. Use of Nanomaterials in Cryobiology and Cryomedicine. Probl Cryobiol Cryomed 2020; 30(4):313-330 https://doi.org/10.15407/cryo30.04.313
Larner SF, Wang J, Goodman J et al. In vitro neurotoxicity resulting from exposure of cultured neural cells to several types of nanoparticles. J Cell Death. 2017 Mar 23; 10:1179670717694523. https://doi.org/10.1177/1179670717694523
Goodarz S, Da Ros T, Conde J, Sefat F, Mozafari M. Fullerene: biomedical engineers get to revisit an old friend Materials Today. 2017; 20(8):460-80 https://doi.org/10.1016/j.mattod.2017.03.017
Andrievsky GV, Klochkov V, Derevyanchenko L. Is C60 fullerene molecule toxic? // Fuller. Nanotub. Carbon Nanostruct. 2005; (13): 363-376. https://doi.org/10.1080/15363830500237267
Paresh Chandra Ray, Hongtao Yu, Peter P Fu Toxicity and Environmental Risks of Nanomaterials: Challenges and Future Needs J Environ Sci Health C Environ Carcinog Ecotoxicol Rev. 2009; 27(1):1-35. https://doi.org/10.1080/10590500802708267
Kumar V, Dasgupta N, Ranjan S. Environmental Toxicity of Nanomaterials, CRC Press; 2018. 537 p. https://doi.org/10.1201/9781351252966
Sforzini S, Oliveri C, Barranger A, Jha AN, Banni M, Moore MN, Viarengo A. Effects of fullerene C60 in blue mussels: Role of mTOR in autophagy related cellular/tissue alterations Chemosphere. 2020; (246):11-3. https://doi.org/10.1016/j.chemosphere.2019.125707
Grynyuk ІІ, Prylutska SV, Slobodyanik NS, Chunikhin ОYu, Matyshevska ОP. The aggregate state Of c60-fullerene in various medi. Biotechnologia acta, 2013; 6(6):71-6.
Kavok N, Grygorova G, Klochkov V, Yefimova. The role of serum proteins in the stabilization of colloidal LnVO4:Eu3+ (Ln = La, Gd, Y) and CeO2 nanoparticles. Colloids and Surfaces. 2017; (529):594-599. https://doi.org/10.1016/j.colsurfa.2017.06.052
Ivanov VK, Shcherbakov AB, Usatenko AV. Structure-sensitive properties and biomedical applications of nanodispersed cerium dioxide. Russ chem rew. 2009; 79(8): 924-43 https://doi.org/10.1070/RC2009v078n09ABEH004058
Shcherbakov АB, Zholobak NМ, Ivanov VК, Tretyakov YuD, Spivak NYa. Nanomaterials based on the nanocrystalline ceric dioxode: properties and use perspectives in biology and medicine. Biotechnologia Acta. 2011; 4(1):9-28.
Shirinkin SV, Volkova TO, Shaposhnikov AA, Andreivskiy GV, Davidovskiy AG. Hydrated fullerene as an instrument to understand the role of special structural characteristics of aqueous medium of a living organism for its normal functioning. Belgorod State University. Scientific Bulletin. Natural sciences. 2012; 128(9):34-50. Russian.
Niu J, Wang K, Kolattukudy PE.Cerium oxide nanoparticles inhibit oxidative stress and nuclear factor-кB activation in H9c2 cardiomyocytes exposed to cigarette smoke extract. J Pharmacol Exp Ther. 2011; 338(1):53-61. https://doi.org/10.1124/jpet.111.179978
Gudkov SV, Guryev EL, Gapeyev B, Sharapov MG, Bunkin NF, Alexey V Shkirin AV, et al. Unmodified hydrated С60 fullerene molecules exhibit antioxidant properties, prevent damage to DNA and proteins induced by reactive oxygen species and protect mice against injuries caused by radiation-induced oxidative stress. Nanomedicine: Nanotechnology, Biology, and Medicine. 2019; (15):37-46 https://doi.org/10.1016/j.nano.2018.09.001
Colon J, Herrera L, Smith J, Patil S, Komanski C, Kupelian P, Seal S, Jenkins DW, Baker CH Protection from radiation-induced pneumonitis using cerium oxide nanoparticles. Nanomedicine. 2009; 5(2):225-31 https://doi.org/10.1016/j.nano.2008.10.003
Fujita N., Kamada K. Protective effect of Ce02 nanoparticles on photo-induced oxidative damage of DNA. Journal of the Ceramic Society of Japan. 2014; (122):141-5 https://doi.org/10.2109/jcersj2.122.141
Kong L, Cai X, Zhou X, Wong LL, Karakoti AS, Seal S, McGinnis JF. Nanoceria extend photoreceptor cell lifespan in tubby mice by modulation of apoptosis/survival signaling pathways. Neurobiol Dis. 2011; 42(3):514-23. https://doi.org/10.1016/j.nbd.2011.03.004
Kartal H, Küçük A, Kiliçarslan A, Polat Y, Süngü N, Kip G, Arslan M. The effect of fullerenol C60 on skeletal muscle after lower limb ischemia reperfusion injury in streptozotocin-induced diabetic rats. J Surg Med. 2020; 4:451-5, https://doi.org/10.28982/josam.756665
Nozdrenko D, Matvienko T, Vygovska O, Bogutska K, Motuziuk O, Nurishchenko N,et al. Protective effect of water-soluble C60 fullerene nanoparticles on the ischemia-reperfusion injury of the muscle soleus in rats. Int. J. Mol. Sci. 2021; 22(13):6812. https://doi.org/10.3390/ijms22136812
Yablonskaya IO, Voeikov VL. Effect of hydrated C60 fullerenes on enzyme activity (in Russian) In: Proceedings of III Eurasian Congress on Medical Physics and Engineering "Medical Physics - 2010". 21-25 of June, 2010 At: Moscow V.3. p. 311-313.
Popov AL, Popova NR, Ivanov VK. Cerium oxide nanoparticles are not toxic for human mesenchymal stem cells and are able to stimulate their proliferation in vitro (in Russian) In: Proceedings of XI International Science-Technical Conference BPPC - 2016. 25-29 of April, 2016 At: Sevastopol V.2, p. 47-51.
Zholobak NM, Lysenko TG. Cyanobacterium Synechococcus cedrorum - the test-culture for toxic bioindication of new chemically synthesized medicinal substances. Reports of the National Academy of Sciences of Ukraine. 2009; (7):123-5. Russian.
Chen K. L., Elimelech M. Aggregation and deposition kinetics of fullerene (C60) nanoparticles. Langmuir. 2006; (22):10994-11001, https://doi.org/10.1021/la062072v
Novikov KN, Berdnikova NG, Novikov AK, Lyusina OY, Muhitova OG, Yablonskaya OI, Minh HD, Voeikov VL. Changes in chemiluminescence of whole blood of COPD patients treated with Hypoxen® and effects of C60 fullerenes on blood chemiluminescence. Med Sci Monit. 2012; 18(2):76-83. https://doi.org/10.12659/MSM.882460
Popov AL, Popova NR, Selezneva II, Ivanov VK. Modulation of the expression of antioxidant enzymes and proinflammatory cytokines by cerium oxide nanoparticles in vitro. European Journal of Human Genetics. 2015; 23(1):450.
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