Hepato and Nephro-protective Potentials of Gongronema latifolium in Streptozotocin Induced diabetic Rat Model
Abstract
Gongronema latifolium (GL) is reported to have hepato-protective and nephron-protective ability. Diabetes is associated with liver and kidney damage. This work was designed to evaluate the effect of ethanolic leaf extract of Gongronema latifolium on liver and kidney functions in Streptozotocin-induced diabetic rats. 40 Wistar rats of both sexes (150g-200g) were divided into five (n=8). Group 1- normal control (received normal saline placebo orally). Group 2 received 200mg/kg GL orally. Group 3 was the diabetic group administered 65mg/kg of streptozotocin (STZ) intraperitoneally for two consecutive days with an interval of one day in between. Group 4 (diabetic + GL group) had STZ (65mg/kg i.p.) + oral administration of 200mg/kg GL. Group 5 was the diabetic group treated with insulin subcutaneously. Blood samples were collected via cardiac puncture to assess Liver enzymes activity, Serum electrolytes biomarkers, Urea and Creatinine. The Result shows a significant (P<0.05) increase in the glucose level of the diabetic group compared to the control. Intervention with GL and insulin significantly reduced the glucose level towards normal. The liver enzymes activity was significantly (p<0.05) increased in the diabetic group but was significantly (p<0.01) decreased following treatment with GL and insulin. Sodium, potassium, chloride, urea and creatinine were significantly (p<0.01) increased in the diabetic group with a decrease in bicarbonate. GL and insulin significantly (p<0.01) reversed these changes in electrolytes and liver enzymes activity towards normal. Results presented shows that GL just like insulin possess hypoglycemic, nephro and hepato-protective potential. Thus, in diabetic condition, Gongronema latifolium leaf extract may exert ameliorative effect on these target organs.
Keywords: Gongronema Latifolium, Diabetes, insulin, serum electrolytes, liver enzymes, rats.
Keywords:
Gongronema Latifolium, Diabetes, insulin, serum electrolytes, liver enzymes, ratsDOI
https://doi.org/10.22270/jddt.v12i5.5623References
American Diabetes Association. Diagnosis and classification of diabetes mellitus. Diabetes care 2013; 36:(Suppl 1):S67-74. https://doi.org/10.2337/dc13-S067
Kumar S, Behl T, Sachdeva M, Sehgal A, Kumari S, Kumar A, Kaur G, Yadav HN, Bungau S. Implicating the effect of ketogenic diet as a preventive measure to obesity and diabetes mellitus. Life Sci 2021; 264:118661. https://doi.org/10.1016/j.lfs.2020.118661
Maher A, Jermeen EY, Manar SM, Amira H. Effect of Ultrasound Cavitation on Weight Reduction for Prediabetic Obese Patients. The Medical Journal of Cairo University 2019; 87: 909-917 https://doi.org/10.21608/mjcu.2019.52713
Barron E, Bakhai C, Kar P, Weaver A, Bradley D, Ismail H, Knighton P, Holman N, Khunti K, Sattar N, Wareham NJ. Associations of type 1 and type 2 diabetes with COVID-19-related mortality in England: a whole-population study. The Lancet Diab Endocrin 2020; 8(10):813-822. https://doi.org/10.1016/S2213-8587(20)30272-2
Chan JC, Lim LL, Wareham NJ, Shaw, JE, Orchard TJ, Zhang P, Lau ES, Eliasson B, Kong AP, Ezzati M, Gregg EW. The Lancet Commission on diabetes: using data to transform diabetes care and patient lives. The Lancet 2021; 396(10267): 2019-2082. https://doi.org/10.1016/S0140-6736(20)32374-6
Chen H, Li R. Introduction of Diabetes Mellitus and Future Prospects of Natural Products on Diabetes Mellitus. In Structure and Health Effects of Natural Products on Diabetes Mellitus, Springer, Singapore, 2021; 1-15. https://doi.org/10.1007/978-981-15-8791-7_1
Chung SS, Ho EC, Lam KS, Chung SK. Contribution of polyol pathway to diabetes-induced oxidative stress. J Ame Soc Nephrol 2003; 14(suppl 3): S233-S236. https://doi.org/10.1097/01.ASN.0000077408.15865.06
Mora C, Navarro JF. Inflammation and diabetic nephropathy. Current Diabetes Reports 2006; 6(6):463-468. https://doi.org/10.1007/s11892-006-0080-1
Green ME, Shah BR, Slater M, Khan S, Jones CR, Walker JD. Monitoring, treatment and control of blood glucose and lipids in Ontario First Nations people with diabetes. CMAJ 2020; 192(33): E937-E945. https://doi.org/10.1503/cmaj.191039
Wild S, Roglic G, Green A, Sicree R, King H. Global prevalence of diabetes: estimates for the year 2000 and projections for 2030. Diabetes Care 2004; 27(5):1047-1053. https://doi.org/10.2337/diacare.27.5.1047
Okpala JC, Igwe JC, Ifedilichukwu HN. Effects of N-Butanol fraction of Gongronema Latifolium leave extract on some liver function and histological parameters in CCl4-induced oxidative damage in wistar albino rats. Biochem Pharm 2014a; 3(126):2167-2171. https://doi.org/10.4172/2167-0501.1000126
Okpala JC, Sani I, Abdullahi R, Ifedilichukwu HN, Igwe JC. Effects of n-butanol fraction of Gongronema latifolium leave extract on some biochemical parameters in CCl4-induced oxidative damage in Wistar albino rats. Afr J Biochem Res 2014b; 8(2):52-64. https://doi.org/10.5897/AJBR2013.0741
Ugochukwu NH. Babady NE. Antioxidant effects of Gongronema Latifolium in hepatocytes of rat models of non-insulin dependent diabetes mellitus. Fitoterapia 2002; 73(7):612-618. https://doi.org/10.1016/S0367-326X(02)00218-6
Constance N, Joy DN, Nkeiruka OA, Ahamefula E, Chidimma A, Oluchi AA. Protective Effects of Bi-Herbal Formulation of Aqueous Extracts of Vernonia amygdalina and Gongronema latifolium against Gentamicin Induced Nephrotoxicity and Liver Injury in Rats. Asian J Res Biochem 2020; 7(4):12-20. https://doi.org/10.9734/ajrb/2020/v7i430144
Ugochukwu NH, Babady NE, Cobourne M, Gasset SR The effect of Gongronema Latifolium leaf extract on serum lipid profile and oxidative stress of hepatocytes of diabetic rats. J BioscI 2003; 28:2-4. https://doi.org/10.1007/BF02970124
Morebise O. (2015). A review on Gongronema Latifolium, an extremely useful plant with great prospects. Eur J Med Plants 2015; 10(1):1-9. https://doi.org/10.9734/EJMP/2015/19713
Ojiako OA, Chikezie PC. Blood Na+/K+ and Cl-Levels of Hyperglycemic Rats Administered with Traditional Herbal Formulations. Pharmacogn Commun 2015; 5:140-145. https://doi.org/10.5530/pc.2015.2.5
Hossain MA, Mostofa M, Debnath D, Alam AK Yasmin Z, Moitry NF. Antihyperglycemic and antihyperlipidemic of Karala (Momordica charantia) fruits in streptozotocin induced diabetic rats. J Environ Sci Nat Res 2012; 5(1):29-37. https://doi.org/10.3329/jesnr.v5i1.11550
Javed F, Al‐Askar M, Al‐Hezaimi K. Cytokine profile in the gingival crevicular fluid of periodontitis patients with and without type 2 diabetes: a literature review. J Periodontology 2012; 83(2):156-161. https://doi.org/10.1902/jop.2011.110207
Schindhelm RK, Diamant M, Heine RJ. Nonalcoholic fatty liver disease and cardiovascular disease risk. Current Diabetes Reports 2007; 7(3):181-187. https://doi.org/10.1007/s11892-007-0030-6
Mathur S, Mehta DK, Kapoor S, Yadav S. Liver function in type-2 diabetes mellitus patients. Int J Sci Study, 2016; 3(10):43-47.
Deeds MC, Anderson JM, Armstrong AS, Gastineau DA, Hiddinga HJ, Jahangir A, Eberhardt NL, Kudva YC. Single dose streptozotocin-induced diabetes: considerations for study design in islet transplantation models. Lab animals 2012; 45(3):131-140. https://doi.org/10.1258/la.2010.010090
Eleazu CO, Eleazu KC, Chukwuma S, Essien UN. Review of the mechanism of cell death resulting from streptozotocin challenge in experimental animals, its practical use and potential risk to humans. J Diab Metc Dis 2013; 12(1):60. https://doi.org/10.1186/2251-6581-12-60
Reitman S, Frankel S. Determination of aminotransaminases in serum. Ame J Clin Path 1957; 2(8):50-56. https://doi.org/10.1093/ajcp/28.1.56
Egbuonu ACC, Obidoa O, Ezeokonkwo CA, Ejikeme PM. Hepatotoxic effects of low dose oral administration of monosodium glutamate in male albino rats. Afri J Biotechn: 2009; 8(13):3031-3035. https://doi.org/10.4314/br.v7i1.45475
King EJ, Armstrong AR. Can Med Assoc J 1964; 31:376 https://doi.org/10.1007/BF02762226
Harris, D. C. (1995). Quantitative Chemical Analysis 4th Ed., New York. Freeman, W. H and Company.
Schales O, Schales. SS. A simple and accurate method for the determination of chloride in biological fluids. J Bioll Chem 1941; 140:879-884. https://doi.org/10.1016/S0021-9258(18)72872-X
Anrade DE. Determination of bicarbonate using back titration. Am. Chem. Soc, 1995; 35:49-54.
Asiwe JN, Daubry TME, Okon IA, Akpotu AE, Adagbada EO, Eruotor H, Agbugba LC, Buduburisi BR. Ginkgo biloba Supplement Reverses Lead (II) Acetate-Induced Haematological Imbalances, Hepatic and Renal Dysfunctions in Male Wistar Rat. Biol Trace Element Res 2022; 1-11. https://doi.org/10.1007/s12011-022-03098-6
Rhoda KM, Porter MJ, Quintini C. Fluid and electrolyte management: putting a plan in motion. J Parenteral Enteral Nutri 2011; 35(6):675-685. https://doi.org/10.1177/0148607111421913
Usoh IF, Akpan HD, Akpanyung EO. Nephroprotection against streptozotocin diabetes is more effective in combined than single leaves extracts of Gongronema Latifolium and Ocimum Gratissimum L. J nnovations Pharma Biol Sci 2016; 3(1):001-016.
Akinsola, A. F., Osasona, I., Akintayo, E. T., Siyanbola, T. O., & Omosebi, S. O. Nutritional Evaluation of Calabash Gourd (L agenaria Siceraria) Seeds and Oil. Journal of Culinary Science & Technology, 2022; 1-22. https://doi.org/10.1080/15428052.2021.2016527
Egbung GE, Essien NA, Mgbang JE, Egbung JE. Serum lipid and electrolyte profiles of Wistar rats fed with Vernonia amygdalina supplemented Vigna subterranea (Bambara groundnut) pudding. Calabar J Health Sci 2020; 3(2):40-45. https://doi.org/10.25259/CJHS_10_2019
Eneji EE, Atangwho IJ, Iwara IA, Eyong UE. Micronutrient and phyto-chemical composition of root bark and twig extracts of Gongronema Latifolium. J Med Med Sci 2011; 2(11):1185-1188.
Akpanabiatu MI, Umoh IB, Udosen EO, Udoh AE, Edet EE. Rat serum electrolytes, lipid profile and cardiovascular activity on Nauclea latifolia leaf extract administration. . Indian J Clin Biochem 2005b; 20(2):29-34. https://doi.org/10.1007/BF02867397
Imo C, Friday OU. Renal Protective Effect of Ethanolic Leaf Extract of Gongronema Latifolium Benth in Acetaminophen-induced Renal Toxicity in Male Albino Rats. Am Chem Sci J 2015; 8(3):1-10. https://doi.org/10.9734/ACSJ/2015/18881
Thapa BR, Walia A. Liver function tests and their interpretation. The Indian J Pediatrics 2007; 74(7):663-671. https://doi.org/10.1007/s12098-007-0118-7
Ajiboye BO, Oyinloye BE, Udebor EA, Owolabi OV, Ejeje JN, Onikanni SA, Omotuyi OI. Hepatoprotective potential of flavonoid-rich extracts from Gongronema latifolium benth leaf in type 2 diabetic rats via fetuin-A and tumor necrosis factor-alpha. Mol Biol Rep 2022; 1-10. https://doi.org/10.1007/s11033-022-07657-x
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