Effect of Time and Haemolysis on Prothrombin Time (PT) and Activated Partial Thromboplastin Time (APTT) Measurement on Blood Samples
Abstract
Background: Coagulation, also known as blood clotting, is the process by which blood convert from a liquid to a gel, forming a blood clot. It referred to haemostasis, the stopping of blood loss from a damaged vessel, followed by repair.
Material and methods: This was cross sectional study conducted at the albawasla medical laboratory, Khartoum, Sudan during the period August to November, 2021 and to evaluate the effect of time and hemolysis on prothrombin time and activated partial thromboplastin time tests. 50 samples (case group) were collected from the patients attending police teaching hospital and requested to the PT and APTT test in addition to that,50 apparently healthy donors with no history of any coagulation problems or any chronic disease were selected as control group. Three ml of venous blood samples were collected in container with Tri Sodium Citrate anticoagulant. The coagulation tests (PT and APTT) were performed using semiautomatic device (coagulometer machine MI).
Results: The result of this study revealed that; when compared the measurement of PT and APTT immediately and after one hour there was insignificant differences (p. v.>0.05). also when compared the measurement of PT and APTT between hemolyzed and non-hemolyzed samples there was significant differences ( p. v.<0.05) in addition when compared case and control for the PT and APTT immediately, after one hour, hemolyzed and non-hemolyzed sample there was significant differences ( p. v.<0.05) except the APTT hemolyzed samples and insignificant differences with age and gender ( p. v.>0.05). For the correlation there was significant correlation in the case group for the PT and APTT immediately, after one hour, and hemolyzed samples.
Conclusion: In the cases group results showed insignificant differences in the results of PT and APTT between immediate sample and after 1 hour in and significant differences in the results of PT and APTT between hemolyzed and non-hemolyzed samples, also there was insignificant differences between age and gender, immediately, after one hr. and hemolyzed sample in PT and APTT.
Keywords: Homeostasis, hemolyzed sample, PT and APTT
Keywords:
Homeostasis, hemolyzed sample, PT, APTTDOI
https://doi.org/10.22270/jddt.v11i6-S.5252References
-Furie B, Furie BC. "Thrombus formation in vivo". The Journal of Clinical Investigation. 2005; 115(12):3355-62. PMC 1297262. PMID 16322780. https://doi.org/10.1172/JCI26987
- David Lillicrap; Nigel Key; Michael Makris; Denise O'Shaughnessy Practical Haemostasis and Thrombosis. Wiley-Blackwell.2009. pp. 1-5. ISBN 978-1-4051-8460-1.
-Chawla R, Goswami B, Singh B, Chawla A, Gupta VK, Mallika V. Evaluating laboratory performance with quality indicators. Lab Med. 2010; 41:297-300. https://doi.org/10.1309/LMS2CBXBA6Y0OWMG
Laga AC, Cheves TA, Sweeney JD. The effect of specimen haemolysis on coagulation test results. Am J Clin Pathol. 2006; 126:748-55. https://doi.org/10.1309/03FK3378YTRA1FRF
Lippi G, Guidi GC. Effect of specimen collection on routine coagulation assays and D-dimer measurement. Clin Chem. 2004; 50:2150-2. https://doi.org/10.1373/clinchem.2004.036244
Bauer NB, Eralp O, Moritz A. Effect of haemolysis on canine kaolin-activated thromboelastography values and ADVIA 2120 platelet activation indices. Vet Clin Pathol. 2010; 39:180-9. https://doi.org/10.1111/j.1939-165X.2010.00224.x
Marlar RA, Potts RM, Marlar AA. Effect on routine and special coagulation testing values of citrate anticoagulant adjustment in patients with high hematocrit values. Am J Clin Pathol. 2006; 126:400-5. https://doi.org/10.1309/RRQKT2JEYV33D19D
van Geest-Daalderop JH, Mulder AB, Boonman-de Winter LJ, Hoekstra MM, van den Besselaar AM. Preanalytical variables and off-site blood collection: Influences on the results of the prothrombin time/international normalized ratio test and implications for monitoring of oral anticoagulant therapy. Clin Chem. 2005; 51:561-8. https://doi.org/10.1373/clinchem.2004.043174
Lippi, G., Guidi, G.C., Mattiuzzi, C. and Plebani, Preanalytical Variability: The Dark Side of the Moon in Laboratory Testing. Clinical Chemistry and Laboratory Medicine, 2006; 44:358-365. https://doi.org/10.1515/CCLM.2006.073
Zhao, Y. and Lv, G. Influence of Temperature and Storage Duration on Measurement of Activated Partial Thromboplastin Time, D-Dimers, Fibrinogen, Prothrombin Time and Thrombin Time, in Citrate-Anticoagulated Whole Blood Specimens. International Journal of Laboratory Haematology, 2013; 35:566-570. https://doi.org/10.1111/ijlh.12113
Burns ER, Yoshikawa N. Homolysis in serum samples drawn by emergency department personnel versus laboratory phlebotomists. Lab Med. 2002; 33:378-80. https://doi.org/10.1309/PGM4-4F8L-2P1M-LKPB
Favaloro EJ, Funk DM, Lippi G. Pre-analytical variables in coagulation testing associated with diagnostic errors in haemostasis. Lab Med 2012; 43:1-0 https://doi.org/10.1309/LM749BQETKYPYPVM
Kroll MH, Elin RJ. Interference with clinical laboratory analyses. Clin Chem. 1994; 40:1996-2005. https://doi.org/10.1093/clinchem/40.11.1996
Published
Abstract Display: 916 
PDF Downloads: 765 PDF Downloads: 884 How to Cite
Issue
Section
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0). that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
How to Cite
.