Antioxidant and Antidiabetic activity of hydroalcoholic flower extract of Woodfordia fruticosa (L.) Kurz
Abstract
Woodfordia fruticosa (L.) Kurz, (W. fruticosa) a member of the Lythraceae family, commonly known as dhaiphul, has been used in traditional medicine to cure common illnesses since ancient times. The most effective part of this plant is the flower which has a high demand in the national as well as the international market of the eastern south part of Asian countries. The present study focuses on exploring the presence of phytochemicals, antioxidants, and antidiabetic potential of hydroalcoholic extract of flower of W. fruticosa. Phytochemical test showed the presence of saponin, flavonoid, carbohydrates, alkaloids, tannin, anthraquinone, and triterpenoids in the extract, and it showed an effective result of total phenolic content, total flavonoid Content with the value of 371.96 ± 2.86 mg of gallic acid equivalent per gram of the extract and 113.82 ± 0.178 mg of quercetin equivalent per gram of dry weight. In vitro antioxidant assay, for the DPPH assay, the IC50 values were found 30.85 ± 0.36 μg/ml and 22.35 ± 0.54 μg/ml for the extract of W. fruticosa flower and ascorbic acid respectively and for the FRAP Assay, the reducing power assay of plant extract is lesser than standard (Ascorbic acid). W. fruticosa also showed significant hyperglycemic activity. In accordance with results, we can conclude that plant consists of a high polyphenolic and flavonoid group which help in antioxidant activity as well as the hydroalcoholic extract shows mild activity against diabetes.
Keywords: Woodfordia fruticosa, phytochemical, hydroalchoholic, antioxidants, antidiabetic, DPPH.
Keywords:
Woodfordia fruticosa, phytochemical, hydroalchoholic, antioxidants, DPPHDOI
https://doi.org/10.22270/jddt.v12i1.5247References
Ekor M. The growing use of herbal medicines: issues relating to adverse reactions and challenges in monitoring safety. Frontiers in pharmacology, 2014; 10(4):177. DOI: https://doi.org/10.3389/fphar.2013.00177
Saxena M, Saxena J, Nema R, Singh D, Gupta A. Phytochemistry of medicinal plants. Journal of pharmacognosy and phytochemistry. 2013; 1:1(6).
Chen J, Mangelinckx S, Adams A, Wang ZT, Li WL, De Kimpe N. Natural flavonoids as potential herbal medication for the treatment of diabetes mellitus and its complications. Natural product communications. 2015; 10(1):187-200. DOI: https://doi.org/10.1177/1934578X1501000140
GBIF (Global Biodiversity Information Facility). GBIF secretariat: GBIF backbone taxonomy. available at: https://www.gbif.org/species/5635525. Accessed December 5, 2021.
Das PK, Goswami S, Chinniah A, Panda N, Banerjee S, Sahu NP, Achari B. Woodfordia fruticosa: Traditional uses and recent findings. Journal of Ethnopharmacology. 2007 Mar 21; 110(2):189-99. DOI: https://doi.org/10.1016/j.jep.2006.12.029
Baravalia Y, Vaghasiya Y, Chanda S. Brine shrimp cytotoxicity, anti-inflammatory and analgesic properties of Woodfordia fruticosa Kurz flowers. Iranian journal of pharmaceutical research: IJPR. 2012; 11(3):851
Khan IA, Singh A, Mindala DP, Meena S, Vij B, Yadav AK, Roy S, Nandi U, Katare AK, Jaglan S, Singh D. Preclinical development of gastro-protective botanical candidate from Woodfordia fruticosa (Linn.) Kurz: Chemical standardization, efficacy, pharmacokinetics and safety pharmacology. Journal of ethnopharmacology. 2019 Sep 15; 241:112023. DOI: https://doi.org/10.1016/j.jep.2019.112023
Chandan BK, Saxena AK, Shukla S, Sharma N, Gupta DK, Singh K, Suri J, Bhadauria M, Qazi GN. Hepatoprotective activity of Woodfordia fruticosa Kurz flowers against carbon tetrachloride induced hepatotoxicity. Journal of ethnopharmacology. 2008 Sep 26; 119(2):218-24. DOI: https://doi.org/10.1016/j.jep.2008.06.020
Chaturvedi PA, Ghatak AA, Desai NS. Evaluation of radical scavenging potential and total phenol content in Woodfordia fruticosa from different altitudes. Journal of plant biochemistry and biotechnology. 2012 Jan; 21(1):17-22. DOI: https://doi.org/10.1007/s13562-011-0066-1
Raghuvanshi R, Nuthakki VK, Singh L, Singh B, Bharate SS, Bhatti R, Bharate SB. Identification of Plant-based Multitargeted Leads for Alzheimer's Disease: In-vitro and In-vivo Validation of Woodfordia fruticosa (L.) Kurz. Phytomedicine. 2021 Jul 21:153659. DOI: https://doi.org/10.1016/j.phymed.2021.153659
Verma N, Amresh G, Sahu PK, Mishra N, Rao CV, Singh AP. Wound healing potential of flowers extracts of Woodfordia fruticosa Kurz. Indian journal of biochemistry and biophysics. 2013 Aug; 50:296-304.
Grover N, Meena R, Patni V. Physiochemical evaluation, phytochemical screening and chromato-graphic fingerprint profile of Woodfordia fruticosa (L.) Kurz extracts. International Journal of Pharmaceutical Sciences and Research. 2014 Jul 1; 5(7):2772.
Yadav M, Chatterji S, Gupta SK, Watal G. Preliminary phytochemical screening of six medicinal plants used in traditional medicine. Int J Pharm Pharm Sci. 2014; 6(5):539-42.
Ikram EH, Eng KH, Jalil AM, Ismail A, Idris S, Azlan A, Nazri HS, Diton NA, Mokhtar RA. Antioxidant capacity and total phenolic content of Malaysian underutilized fruits. Journal of food Composition and Analysis. 2009 Aug 1; 22(5):388-93. DOI: https://doi.org/10.1016/j.jfca.2009.04.001
Aryal S, Baniya MK, Danekhu K, Kunwar P, Gurung R, Koirala N. Total phenolic content, flavonoid content and antioxidant potential of wild vegetables from Western Nepal. Plants (Basel). 2019 Apr 11; 8(4):96. DOI: https://doi.org/10.3390/plants8040096
Mensor LL, Menezes FS, Leitão GG, Reis AS, Santos TC, Coube CS, Leitão SG. Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytotherapy research. 2001 Mar; 15(2):127-30. DOI: https://doi.org/10.1002/ptr.687
Oyaizu M. Studies on products of browning reaction antioxidative activities of products of browning reaction prepared from glucosamine. The Japanese journal of nutrition and dietetics. 1986; 44(6):307-15.
Apostolidis E, Lee CM. In vitro potential of Ascophyllum nodosum phenolic antioxidant‐ mediated α‐glucosidase and α‐amylase inhibition. Journal of food science. 2010 Apr; 75(3):H97-102. DOI: https://doi.org/10.1111/j.1750-3841.2010.01544.x
Shu XS, Lv JH, Tao J, Li GM, Li HD, Ma N. Antihyperglycemic effects of total flavonoids from Polygonatum odoratum in STZ and alloxan-induced diabetic rats. Journal of ethnopharmacology. 2009 Jul 30; 124(3):539-43. DOI: https://doi.org/10.1016/j.jep.2009.05.006
Mechchate H, Es-Safi I, Louba A, Alqahtani AS, Nasr FA, Noman OM, Farooq M, Alharbi MS, Alqahtani A, Bari A, Bekkari H. In Vitro Alpha-Amylase and Alpha-Glucosidase Inhibitory Activity and In Vivo Antidiabetic Activity of Withania frutescens L. Foliar Extract. Molecules. 2021 Jan; 26(2):293. DOI: https://doi.org/10.3390/molecules26020293
Alqahtani AS, Hidayathulla S, Rehman MT, ElGamal AA, Al-Massarani S, Razmovski-Naumovski V, Alqahtani MS, El Dib RA, AlAjmi MF. Alpha-Amylase and alpha-glucosidase enzyme inhibition and antioxidant potential of 3-Oxolupenal and Katononic Acid Isolated from Nuxia oppositifolia. Biomolecules. 2020 Jan; 10(1):61. DOI: https://doi.org/10.3390/biom10010061
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