Design, Development and Characterization of Nanoemulsion developed by High Pressure Homogenization (HPH) method Containing Antifungal Drug
Nanoemulsion is one the most attractive drug delivery for researchers to treat fungal diseases and getting interested in increasing the solubility of low soluble drugs. The objective of this research was to develop a topical nanoemulsion formulation of Posaconazole drug with the intention to enhance its aqueous solubility and local action. By employing High Pressure Homogenization (HPH) method, oil in water nanoemulsion was formulated with 0.2% cinnamon oil as lipid phase, 0.4% tween-80 and poloxamer-188 as surfactants, 0.2% transcutol as a co-surfactant. The physical characteristics of formulations were found to be stable after thermodynamic stability testing. In-vitro diffusion study for optimized nanoemulsion was performed using a dialysis bag membrane and cumulative % drug release was determined. Viscosity and percent drug content was observed to be 0.0593 cps and 90.21 ± 0.23% respectively. The optimized nanoemulsion formulation (F8) was evaluated to be transparent and thermodynamically stable, with -9.46 zeta potential, 78.79 nm particle size, 0.315 polydispersity index. The optimized Nanoemulsion was stable for 3 month in three different temperature conditions. The result from the release study was indicative of improved solubility of Posaconazole, which may serve to boost up the action of the drug for the treatment of fungal diseases.
Keywords: Posaconazole, Nanoemulsion, HPH method, Physical Characterization
2. Jacobsen ID. Fungal infection strategies. Virulence. 2019;10(1):835–8. Available from: https://doi.org/10.1080/21505594.2019.1682248
3. Nagappan V, Deresinski S. Posaconazole: A broad-spectrum triazole antifungal agent. Clin Infect Dis. 2007; 45(12):1610–7.
4. Garg A, Sharma GS, Goyal AK, Ghosh G, Si SC, Rath G. Recent advances in topical carriers of anti-fungal agents. Heliyon. 2020; 6(8):e04663. Available from: https://doi.org/10.1016/j.heliyon.2020.e04663
5. Kassem MA, Ghalwash MM, Abdou EM. Development of nanoemulsion gel drug delivery systems of cetirizine; factorial optimisation of composition, in vitro evaluation and clinical study. J Microencapsulation. 2020; 37(6):413–30.
Available from: http://dx.doi.org/10.1080/02652048.2020.1771446
6. Patel NA, Patel NJ, Patel RP. Formulation and evaluation of curcumin gel for topical application. Pharm Dev Technol. 2009; 14(1):83–92. https://doi.org/10.1080/10837450802409438
7. Kujawski J, Czaja K, Dettlaff K, Żwawiak J, Ratajczak T, Bernard MK. Structural and spectroscopic properties of posaconazole – Experimental and theoretical studies. J Mol Struct.2019;1181:179–89. https://doi.org/10.1016/j.molstruc.2018.12.074
8. More A.,Wahid AA. Development and Characterization of Nanoemulsion Gel for Topical Drug Delivery of Nabumetone. Int. J of Pharmacy & Pharm Research 2016; 7 (3):126-157.
9. Jagdish G, Shukla P, Shukla R.Formulation And Evaluation Of Microemulsion Based Gel of Posaconazole For Topical Delivery Epra International Journal of Research and Development ( IJRD ). 2021; 6(1):164–74.
10. Jayvadan P., Modi J. Nanoemulsion-Based Gel Formulation of Aceclofenac for Topical Delivery Nanoemulsion-Based Gel Formulation of Aceclofenac for Topical Delivery. International Journal of Pharmacy and Pharmaceutical Science Research 2011; 1(1):6-12 11.
11. Hamid KM, Wais M, Sawant G. a Review on Nanoemulsions: Formulation, Composition, and Applications. Asian J Pharm Clin Res. 2021;14(4):22–8. https://doi.org/10.1080/10837450802409438
12. Calligaris S, Plazzotta S, Bot F, Grasselli S, Malchiodi A, Anese M. Nanoemulsion preparation by combining high pressure homogenization and high power ultrasound at low energy densities. Food Res Int. 2016; 83:25–30. Available from: http://dx.doi.org/10.1016/j.foodres.2016.01.033
13. Sharma N, Mishra S, Sharma S, Deshpande RD, Kumar Sharma R. Preparation and Optimization of Nanoemulsions for targeting Drug Delivery. Int J Drug Dev Res. 2013;5(4):37–48.
14. Qian C, McClements DJ. Formation of nanoemulsions stabilized by model food-grade emulsifiers using high-pressure homogenization: Factors affecting particle size. Food Hydrocoll. 2011; 25(5):1000–8. http://dx.doi.org/10.1016/j.foodhyd.2010.09.017
15. Kaur R, Ajitha M. Transdermal delivery of fluvastatin loaded nanoemulsion gel: Preparation, characterization and in vivo anti-osteoporosis activity. Eur J Pharm Sci 2019; 136:104956. https://doi.org/10.1016/j.ejps.2019.104956
16. Lala RR, Awari NG. Nanoemulsion-based gel formulations of COX-2 inhibitors for enhanced efficacy in inflammatory conditions. Appl Nanosci. 2014; 4(2):143–51.
17. Maha HL, Sinaga KR, Masfria. Formulation and evaluation of miconazole nitrate nanoemulsion and cream. Asian J Pharm Clin Res. 2018; 11(3):319–21. https://doi.org/10.1080/10837450802409438
18. Gurpreet K., Singh SK.Review of Nanoemulsion Formulation and Characterization Techniques. Indian Journal of Pharmaceutical Sciences 2018; 80(5) https://doi.org/10.1080/10837450802409438
19. Fernández-Campos F, Clares Naveros B, López Serrano O, Alonso Merino C, Calpena Campmany AC. Evaluation of Novel Nystatin Nanoemulsion for Skin Candidosis Infections. Mycoses. 2013; 56(1):70–81. https://doi.org/10.1111/j.1439-0507.2012.02202.x
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