Chrysin and rutin protect against hydrogen peroxide and tert-butyl hydroperoxide induced oxidative cell damage

  • Rouamba Ablassé Laboratory of Applied Biochemistry and Chemistry (LABIOCA), UFR-SVT, University Joseph KI-ZERBO, 03 BP 7021, Ouagadougou 03, Burkina Faso
  • Compaoré Moussa Laboratory of Applied Biochemistry and Chemistry (LABIOCA), UFR-SVT, University Joseph KI-ZERBO, 03 BP 7021, Ouagadougou 03, Burkina Faso;
  • Ouédraogo Maurice Laboratory of Animal Physiology (LAPA), UFR-SVT, University Joseph KI-ZERBO, 09 BP 848, Ouagadougou 09, Burkina Faso;
  • Bationo Raoul University Hospital Center of Bogodogo, Ouagadougou, Burkina Faso;
  • Kiendrebeogo Martin Laboratory of Applied Biochemistry and Chemistry (LABIOCA), UFR-SVT, University Joseph KI-ZERBO, 03 BP 7021, Ouagadougou 03, Burkina Faso;

Abstract

Objective: Chrysin and rutin are two dietary flavonoids lying in fruits or honey bee’s products. Their pharmacological properties include antioxidant, anti-inflammatory, anticancer, neuroprotection and immunomodulatory. In the current study, the potentiality of chrysin and rutin to protect human gingival fibroblasts against oxidative cell damage has been investigated in vitro.  


Method: Human gingival fibroblasts, passage 3, were concomitantly put in contact with the cytotoxic compounds and chrysin or rutin for 24 h at 37 °C, 5% CO2 atmosphere, and 96% humidity. The amount of viable cell after the incubated time was recorded by using the thiazolyl blue tetrazolium bromide (MTT) assay. 


Results: Chrysin in all tested concentration didn’t exhibit any cytoprotective effect against the tert-butyl hydroperoxide-induced oxidative cell damage. Moreover, chrysin in a low concentration (5 and 10 µg/mL) didn’t protect the fibroblasts against oxidative cell damage induced by the hydrogen peroxide. However, chrysin in a concentration of 20 µg/mL showed a significant cytoprotective activity in the hydrogen peroxide-induced cell damage (p < 0.05). Rutin in all tested concentrations protected fibroblasts against hydrogen peroxide and tert-butyl hydroperoxide-induced oxidative cell damage. The cytoprotective effect of rutin didn’t increase with the increase of the concentration when hydrogen peroxide is used to induce oxidative cell damage. However, rutin has protected cells against the tert-butyl hydroperoxide cytotoxicity in a concentration dependent manner.


Conclusion: Given to the interesting cytoprotective activities exhibited by chrysin and rutin, further investigations to highlight their cytoprotective involved mechanisms are justified.  


Keywords: Chrysin, Cytoprotective, Fibroblasts, Rutin.

Keywords: Cytoprotective, Fibroblasts, Chysin, Rutin

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Author Biographies

Rouamba Ablassé, Laboratory of Applied Biochemistry and Chemistry (LABIOCA), UFR-SVT, University Joseph KI-ZERBO, 03 BP 7021, Ouagadougou 03, Burkina Faso

Laboratory of Applied Biochemistry and Chemistry (LABIOCA), UFR-SVT, University Joseph KI-ZERBO, 03 BP 7021, Ouagadougou 03, Burkina Faso;

Compaoré Moussa, Laboratory of Applied Biochemistry and Chemistry (LABIOCA), UFR-SVT, University Joseph KI-ZERBO, 03 BP 7021, Ouagadougou 03, Burkina Faso;

Laboratory of Applied Biochemistry and Chemistry (LABIOCA), UFR-SVT, University Joseph KI-ZERBO, 03 BP 7021, Ouagadougou 03, Burkina Faso;

Ouédraogo Maurice, Laboratory of Animal Physiology (LAPA), UFR-SVT, University Joseph KI-ZERBO, 09 BP 848, Ouagadougou 09, Burkina Faso;

Laboratory of Animal Physiology (LAPA), UFR-SVT, University Joseph KI-ZERBO, 09 BP 848, Ouagadougou 09, Burkina Faso;

Bationo Raoul, University Hospital Center of Bogodogo, Ouagadougou, Burkina Faso;

University Hospital Center of Bogodogo, Ouagadougou, Burkina Faso;

Kiendrebeogo Martin, Laboratory of Applied Biochemistry and Chemistry (LABIOCA), UFR-SVT, University Joseph KI-ZERBO, 03 BP 7021, Ouagadougou 03, Burkina Faso;

Laboratory of Applied Biochemistry and Chemistry (LABIOCA), UFR-SVT, University Joseph KI-ZERBO, 03 BP 7021, Ouagadougou 03, Burkina Faso;

References

1. Sudha G, Priya MS, Shree RI, Vadivukkarasi S. In vitro free radical scavenging activity of raw pepino fruit (Solanum muricatumatton). Int J Curr Pharm Res. 2011; 3(2):8-11. https://doi.org/10.1111/j.1750-3841.2012.02944.x
2. Manzolli ES, Serpeloni JM, Grotto D, Bastos JK, Maria L, Antunes G, et al. Protective Effects of the Flavonoid Chrysin against Methylmercury-Induced Genotoxicity and Alterations of Antioxidant Status , In Vivo. Oxid Med Cell Longev. 2015; 2015:1-7. https://doi.org/10.1155/2015/602360
3. Li K, Fan H, Yin P, Yang L, Xue Q, Li X, et al. Structure-activity relationship of eight high content flavonoids analyzed with a preliminary assign-score method and their contribution to antioxidant ability of flavonoids-rich extract from Scutellaria baicalensis shoots. Arab J Chem. 2017; 11(35):159-70. https://doi.org/10.1016/j.arabjc.2017.08.002
4. Kumar S, Pandey AK. Chemistry and Biological Activities of Flavonoids : An Overview. Sci World J. 2013; 2013:1-16. https://doi.org/10.1155/2013/162750
5. Moghadam ER, Ang HL, Asnaf SE, Zabolian A. Broad-Spectrum Preclinical Antitumor Activity of Chrysin : Current Trends and Future Perspectives. Biomolecules. 2020; 10(1374):1-39. https://doi.org/10.3390/biom10101374
6. Mehdi SH, Nafees S, Khan A, Rizvi MA. iMedPub Journals Chrysin : A Promising Anticancer Agent its Current Trends and Future Perspectives Bioavailability and Toxicity. Eur J Exp Biol. 2018; 8(3):1-7. https://doi.org/10.21767/2248-9215.100057
7. Ganeshpurkar A, Saluja AK. The Pharmacological Potential of Rutin. Saudi Pharm J. 2017; 25(2):149-64. https://doi.org/10.1016/j.jsps.2016.04.025
8. Lazaro de SF, Joao AL de M, Conceiçao de SM, Lucianny M, Barbosa L, Bind H, et al. Role of Rutin in 5-Fluorouracil-Induced Intestinal Mucositis : Prevention of Histological Damage and Reduction of Inflammation and Oxidative Stress. molecules. 2020; 25(2786):1-23. https://doi.org/10.3390/molecules25122786
9. Boyle SP, Dobson VL, Duthie SJ, Hinselwood DC, Kyle JAM, Collins AR. Bioavailability and efficiency of rutin as an antioxidant : A human supplementation study. Eur J Clin Nutr. 2000; 54:774-82. https://doi.org/10.1038/sj.ejcn.1601090
10. Su K, Yu CY, Chen Y, Huang Y, Chen C, Wu H, et al. Rutin , a Flavonoid and Principal Component of Saussurea Involucrata , Attenuates Physical Fatigue in a Forced Swimming Mouse Model. Int J Med Sci. 2014; 11(5):528-37. https://doi.org/10.7150/ijms.8220
11. Almutairi MM, Alanazi WA, Alshammari MA, Alotaibi MR, Alhoshani AR, Al-rejaie SS, et al. Neuro-protective effect of rutin against Cisplatin-induced neurotoxic rat model. Complement Altern Med. 2017; 17(472):1-9. https://doi.org/10.1186/s12906-017-1976-9
12. Prasad R, Prasad SB. A review on the chemistry and biological properties of Rutin , a promising nutraceutical agent. Asian J Pharm Pharmacol. 2019; 5:1-20. https://doi.org/10.31024/ajpp.2019.5.s1.1
13. Ramalingayya VG, Cheruku SP, Nayak GP, Kishore A, Chamallamudi SR, Rao M, et al. Rutin protects against neuronal damage in vitro and ameliorates doxorubicin-induced memory deficits in vivo in Wistar rats. Drug Des Devel Ther. 2017;1 1:1011-26. https://doi.org/10.2147/DDDT.S103511
14. Bationo R, Rouamba A, Diarra A, Beugré-kouassi ML, Jordana F, Beugré J. Culture of Human Gingival Fibroblasts : An Experimental Model. Cell Biol. 2020; 8(1):8-11. https://doi.org/10.11648/j.cb.20200801.12
15. World Medical Association W. World Medical Association Declaration of Helsinki Ethical Principles for Medical Research Involving Human Subjects. J Am Med Assoc. 2013; 310(20):2191-4. https://doi.org/10.1001/jama.2013.281053
16. Burlec AF, Pecio Ł, Kozachok S, Mircea C, Corciov A, Vere L, et al. Phytochemical Profile , Antioxidant Activity , and Cytotoxicity Assessment of Tagetes erecta L . Flowers. Molecules. 2021; 26(1201):1-15. https://doi.org/10.3390/molecules26051201
17. Halliwell B. Biochemistry of oxidative stress. Biochem Soc Trans. 2007; 35(5):1147-50. https://doi.org/10.1042/BST0351147
18. Bota DA, Davies KJA. Protein degradation in mitochondria : implications for oxidative stress , aging and disease : a novel etiological classi ® cation of mitochondrial proteolytic disorders. Mitochondrion. 2001; 1(1):33-49. https://doi.org/10.1016/S1567-7249(01)00005-8
19. Kelly FJ. OXIDATIVE STRESS : ITS ROLE IN AIR POLLUTION AND ADVERSE HEALTH EFFECTS. Occup Environ Med. 2003; 60:612-6. https://doi.org/10.1136/oem.60.8.612
20. Manisha N, Hasan W, Rajak R, Jat D. Oxidative stress and antioxidants : an overview. Int J Adv Res Rev. 2017; 2(9):110-9.
21. Breitenbach M, Eckl P. Introduction to Oxidative Stress in Biomedical and Biological Research. Biomolecules. 2015; 5:1169-77. https://doi.org/10.3390/biom5021169
22. Gupta RK, Patel AK, Shah N, Kumar A, Jha UK, Yadav UC, et al. Oxidative Stress and Antioxidants in Disease and Cancer : A Review. Asian Pacific J Cancer Prev. 2014; 15:4405-9. https://doi.org/10.7314/APJCP.2014.15.11.4405
23. Barbusinski K. Fenton reaction - Controversy concerning the chemistry. Ecol Chem Eng. 2009; 16(3):347-58.
24. Lipinski B. Hydroxyl Radical and Its Scavengers in Health and Disease. Oxid Med Cell Longev. 2011; 2011:1-9. https://doi.org/10.1155/2011/809696
25. Dizdaroglu M, Jaruga P. Mechanisms of free radical-induced damage to DNA. Free Radic Res. 2012; 46(4):382-419. https://doi.org/10.3109/10715762.2011.653969
26. Borra SK, Mahendra J, Gurumurthy P, Jayamathi, Iqbal SS, Mahendra L. Effect of Curcumin against Oxidation of Biomolecules by Hydroxyl Radicals. J Clin Diagnostic Res. 2016; 8(10):1-5.
27. Kucera O, Endlicher R, Roušar T, Lotková H, Garnol T, Drahota Z, et al. The Effect of tert-Butyl Hydroperoxide-Induced Oxidative Stress on Lean and Steatotic Rat Hepatocytes In Vitro. Oxid Med Cell Longev. 2014; 2014:1-12. https://doi.org/10.1155/2014/752506
28. Makota O, Trach Y, Saldan I, Evers E, Kalevaru VN, Martin A. Decomposition of Tert -Butyl Hydroperoxide in the presence of selected initiators and catalysts. Chem Chem Technol. 2018; 12(2):154-7. https://doi.org/10.23939/chcht12.02.154
29. Khan R, Khan AQ, Qamar W, Lateef A, Ali F, Rehman MU, et al. Chrysin abrogates cisplatin-induced oxidative stress, p53 expression, goblet cell disintegration and apoptotic responses in the jejunum of Wistar rats. Br J Nutr. 2012; 108:1574-85. https://doi.org/10.1017/S0007114511007239
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1.
Ablassé R, Moussa C, Maurice O, Raoul B, Martin K. Chrysin and rutin protect against hydrogen peroxide and tert-butyl hydroperoxide induced oxidative cell damage. JDDT [Internet]. 15Nov.2021 [cited 1Dec.2021];11(6):20-5. Available from: https://jddtonline.info/index.php/jddt/article/view/5131