Trypanocidal effect of aqueous and ethanolic extracts of Strychnos spinosa on white mice infected with Trypanosoma brucei brucei
Abstract
The emergence of trypanocidal resistance has led to a change in the behavior and use of local plants for the treatment of parasites. The trypanocidal activity of Strychnos spinosa aqueous and ethanolic extracts on Trypanosoma brucei brucei was evaluated during ten days of treatment. Thus, the smear was used to monitor the parasitaemia of mice treated with Strychnos spinose aqueous and ethanolic extracts by gavage at doses of 250, 500, 1000 mg/kg (test groups); with 10 ml/kg of distilled water (negative control) and 1mg/kg of isometamidium chloride (positive control). The results of the pharmacological studies on the trypanocidal potential of the plant showed that On day ten (D10) a mortality rates of 32 %, 39 % and 62 % respectively for the doses 250, 500 and 1000 mg/kg with the ethanolic extract while the aqueous extract induced a mortality rates of 32 %, 37 % and 56 % respectively. Isometamidium chloride induced the highest mortality rate of 85 %. The lethal doses were 218.07 and 225.79 mg/kg for the aqueous and ethanolic extracts respectively. All these results justify at least in part the use of this plant in traditional medicine for the treatment of trypanosomiasis.
Keywords: Ethanolic extracts - Aqueous extracts - Trypanocidal potential - Trypanosomes - Inhibition of parasitaemia.
Keywords:
Ethanolic extracts, Aqueous extracts, Trypanocidal potential, Trypanosomes, Inhibition of parasitaemiaDOI
https://doi.org/10.22270/jddt.v11i6-S.5103References
Barro F. Contribution to the Epidemiological Study of Human African Trypanosomiasis in Mali. In the former HAT foci of Kita, Kéniéba, Kolondièba, Yanfolila in the Republic of Mali. Thesis in pharmacy, Bamako, 2003; 137p.
Sissoko MM. Overview of human African trypanosomiasis in Mali: assessment of thirty-seven years of activities (from 1970 to 2007). Thesis Faculty of Medicine, Pharmacy and Odonto-stomatology, 2007; 136p.
Desquesnes M, Michel JF, de la Rocque S, Solano P, Millogo L, Bengaly Z, Sidibe I. Parasitological and serological survey (Elisa-indirect) on bovine trypanosomoses in the Sidéradougou area, Burkina Faso. Journal of Animal Husbandry and Veterinary Medicine in Tropical Countries, 1999; 52(3-4):223-232. https://doi.org/10.19182/remvt.9667
Hamadama H. Control of bovine trypanosomiasis on the Amadaoua plateau in Cameroon. Thesis: Veterinary Medicine Dakar, 1982; 126p.
Sawadogo R. Trypanocidal effect of medicinal plants from Burkina Faso: development of a method for the comparative evaluation of extracts of four plants on cattle trypanosomes in Africa. Doctoral thesis in Pharmacy from the University of Ouagadougou, 2003; 143p.
Barrett MP and Fairlamb AH. The biochemical basis of arsenical-diamidine crossresistance in African trypanosomes. Parasitol Today, 2007; 15(4):136-40. https://doi.org/10.1016/S0169-4758(99)01414-3
Kennedy PG. "The continuing problem of human African trypanosomiasis (sleeping sickness)." Ann Neurol, 2008; 64(2): 116-26. https://doi.org/10.1002/ana.21429
Nikiéma JB. Effects of antiinflammatory Triterpènes isolated from Leptadenia hastata latex on keratinocyte proliferation. Phytother Res, 2001; 15(2):131-134. https://doi.org/10.1002/ptr.700
Nouhoun C. Importance of essential drugs in the cost recovery system of health care in the KBK zone of the PDS. Doctoral thesis in Pharmacy from the University of Bamako Mali, 1988; 88p.
Mamoudou A, Zoli A, Mbahin N, Tanenbe C, Bourdanne A, Clausen PH, Marcotty T, Van den Bossche P, Geerts S. Prevalence and incidence of bovine trypanosomosis on the Adamaoua Plateau in Cameroon 10 years after the tsetse eradication campaign. Vetenary parasitology, 2006; 142:16-22. https://doi.org/10.1016/j.vetpar.2006.06.033
Darsaud A. Study of the circadian dysrhythmia of sleep-wake alternation, clinical follow-up and therapeutic validation in an experimental model of African trypanosomiasis, 2003; p.17.
Diallo D, Doumbia O, Sanogo F, Mahamoud M. Contribution to the phytochemical study of some medicinal plants of Gourma. Annex N° 21, SSE progress report wild plants. Doctoral thesis in pharmacy from the University of Gourma Mali, 1992; 140p.
Boukef MK. Traditional medicine and pharmacopoeia. Plants in traditional Tunisian medicine, 1986. p. 350.
Boullard B. Medicinal plants of the world, realities and beliefs. Editions Estems, 2001. p. 636.
Hennebelle T. Sahpaz S, Bailleul F. Plant polyphenols, sources, uses and potential in the fight against oxidative stress. Phytotherapie, 2004; N°1: 3-6. https://doi.org/10.1007/s10298-004-0003-8
Karumi Y, Onyeyili PA, Ogugbuaja VO. Identification of active principles of M. balsamina (Balsam Apple) leaf extract. J Med Sci., 2004; 4(3):179-182. https://doi.org/10.3923/jms.2004.179.182
Edeoga HO, Okwu DE, Mbaebie BO. Phytochemical constituents of some Nigerian medicinal plants. African Journal of Biotechnology, 2005 ; 4(7):685-688. https://doi.org/10.5897/AJB2005.000-3127
Fatoumata OO. Traditional treatment of sexually transmitted infections in Mali: Study of the phytochemistry and biological activities of Annona senegalensis L. (Annonaceae) and Stchytarpheta angustifolia VALH (Verbenaceae), 2005. p. 165-188.
WHO. Control and surveillance of African trypanosomiasis. Report of a Who Expert Committee. Who technical Report Series, 1998; n°881.
Tamboura HH. Ecological distribution, morphological characteristics and acute toxicity of aqueous extracts of Holarrhena floribunda (G. Don) Durand and Schinz, Leptadenia hastata (Pers.) Decne and Cassia sieberiana (DC) used by veterinary healers in Burkina Faso. Afr. J. Trad. Complementary Altern. Med., 2004; 2:11-22. https://doi.org/10.4314/ajtcam.v2i1.31100
Hilaly JE, Israili ZH, Lyoussi B. Acute and chronic toxicological studies of Ajuga iva in experimental animals. J. Ethnopharmacol, 2004; 91:43-50. https://doi.org/10.1016/j.jep.2003.11.009
Leger S. Strychnos cocculoides Baker, Strychnos pungens Solered. Forsthaus 1, D-85290, Guisenfeld, Germany, 2003. p. 98.
Matovu E, Stewart ML, Geiser F, Brun R, Maser P. Mechanisms of arsenical and diamidine uptake and resistance in Trypanosoma brucei. Eukaryot Cell, 2003; 2(5):1003-8. https://doi.org/10.1128/EC.2.5.1003-1008.2003
Balé B, Maria T, Rubio P, Moussa Z, Benoit M, Laya S. Anti-androgenic activity of Leptadenia hastata (Pers.) Dene: competitive effect of aqueous extracts of the plant and testosterone propionate on impubertal castrated rats, 2010. p. 229.
Vitouley SH. Study of the trypanocidal potential of aqueous extracts of medicinal plants for the treatment of African animal trypanosomosis, 2005. p. 63-81.
Trail JCM, Ieteren GDM, Feron AL. Effect of trypanosome infection, control of parasitemia and control of anernla development on productivity of N'Dama cattle. Acta Tropica, 1991; 48:37-45. https://doi.org/10.1016/0001-706X(90)90063-6
Tamini L. Preliminary pharmacological study of Terminalia avicennioides Guill and Perr. (Combretaceae): action on African animal trypanosomosis. Dissertation of D.E.A.: Applied Biological Sciences : Université de Ouagadougou, 1994 ; 63p.
Ake-Assi YA. Contribution to the census of plant species traditionally used for zootechnical and veterinary purposes in West Africa. PhD thesis: Faculty of Veterinary Sciences, Claude Bernard University (Lyon), 1992; 225p.
Tamboura HH. Ecological distribution, morphological characteristics and acute toxicity of aqueous extracts of Holarrhena floribunda (G. Don) Durand & Schinz, Leptadenia hastata (Pers.) Decne and Cassia sieberiana (DC) used by veterinary healers in Burkina Faso. Afr. J. Trad. Complementary Altern. Med., 2004; 2:11-22. https://doi.org/10.4314/ajtcam.v2i1.31100
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