The Effect of Various Concentrations of Ethanol Extract of the Leaves of Paederia foetida L. on the Growth of Escherichia Coli Bacteria

  • Hertina Silaban Medical Faculty, Universitas Kristen Indonesia, Jakarta, Indonesia

Abstract

Bacterial infection of Escherichia coli (E. coli) as the cause of gastrointestinal disorders in humans has increased their prevalence. Treatment using natural ingredients can be a choice of therapy because of the minimal side effects. One of the rare plants believed by the community as an antibacterial is stinking vin’e known as the ‘leaf fart’. The purpose of this research is for knowing the activity of the ethanol extract of Paederia foetida L can affect the growth of E.coli. The serial diffusion disc method is being used as the antibacterial activity test. The concentration  of this extract are 10%, 20%, 40%, 80%, 100% with positive control (ciprofloxacin) and negative control (aqua dest). The inhibition zone diameter characterized the effect of Extract on bacterial growth were 6.16 mm of the concentration 10%, 6.667 mm of the concentration 20%, 7.10 mm of the concentration 40 %, 7.78 mm of the concentration 80%, and 10.03 mm of the concentration 100%. As for the negative control has no effect. The study stated that the higher concentration of antibacterial agent used, the greater the inhibition zone formed. Based on the result of the analysis of the data by using the One-Way ANOVA Test showed a probability value (p) = 0.000 or value (p) < 0.05, that H0 is rejected and H1 is accepted. The conclusion is that the Extract of stinking vin’e has an antibacterial effect on the growth of E.coli.


Keywords: Antibacterial, E.coli, Extract of  Sembukan leaf

Keywords: Antibacterial, E.coli, Extract of Sembukan leaF

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Author Biography

Hertina Silaban, Medical Faculty, Universitas Kristen Indonesia, Jakarta, Indonesia

Medical Faculty, Universitas Kristen Indonesia, Jakarta, Indonesia

References

1. Sartika, Ratu Ayu Dewi, Yvonne M. Indrawani, and Trini Sudiarti. "Analisis mikrobiologi Escherichia coli O157: H7 pada hasil olahan hewan sapi dalam proses produksinya." Makara Kesehatan 2005; 9(1):23-28
2. Mahon, C., D. Lehman, and George Manuselis. "Textbook of Diagnostic Microbiology. 5th Edidtion." Missouri: Elsevier (2014).
3. Zakki, Ghulam Izza. "Pengetahuan Dan Perilaku Preventif Terhadap Bakteri E-Coli Pada Masyarakat Kecamatan Gondomanan di Kota Yogyakarta." PhD diss., Universitas Negeri Semarang, 2015.
4. Jawetz, Ernest, Joseph L. Melnick, and Edward A. Adelberg. "Jawetz, melnick, Adelberg's Medical microbiology." 2001.
5. Welch, Rodney A. "The genus Escherichia." Prokaryotes 2006; 6(1):60-71 https://doi.org/10.1007/0-387-30746-X_3
6. Cavalieri, S. J., R. J. Harbeck, Y. S. McCarter, J. H. Ortez, I. D. Rankin, R. L. Sautter, S. E. Sharp, and C. A. Spiegel. "Manual of antimicrobial susceptibility testing. American Society for Microbiology." Pan American Health Organization: Washington, DC, USA 2005.
7. KemenKes, R. I. "Riset kesehatan dasar (Riskesdas) 2013." Jakarta: Badan Penelitian dan Pengembangan Kesehatan Kementrian Kesehatan Republik Indonesia 2013.
8. KeMenKes, R. I. "Profil kesehatan Indonesia tahun 2015." Jakarta Kementeri Kesehat Republik Indones 2016.
9. Maharani, Krisnina. "Uji Antibakteri Ekstrak Kulit Buah dan Biji Manggis (Garcinia mangostana) pada Bakteri Penyebab Jerawat (Staphylococcus epidermidis) dengan Menggunakan Solven Etanol." PhD diss., UNIVERSITAS AIRLANGGA, 2012.
10. Nurcahyanti, A. D. R., and J. Wandra. "Sembukan kurang sedap namun berkhasiat hebat." Bios: Salatiga 2012.
11. Handrianto, Prasetyo. "Analisis Kandungan Kimia Daun Dan Batang Sembukan (Paederia Foetida) Dengan Menggunakan 2 Pelarut Yang Berbeda." Journal of Pharmacy and Science 2018; 3(2):23-27. https://doi.org/10.53342/pharmasci.v3i2.112
12. Leininger, Dagny Jayne, Jerry Russel Roberson, and Francois Elvinger. "Use of eosin methylene blue agar to differentiate Escherichia coli from other gram-negative mastitis pathogens." Journal of veterinary diagnostic investigation 2001; 13(3):273-275. https://doi.org/10.1177/104063870101300319
13. Kumari, Preeti, Chandrawati Kumari, and Poornima Shekhar Singh. "Phytochemical screening of selected medicinal plants for secondary metabolites." Int. J. Life. Sci. Scienti. Res 2017; 3(4):1151-1157. https://doi.org/10.21276/ijlssr.2017.3.4.9
14. Saifudin, Azis. Senyawa alam metabolit sekunder teori, konsep, dan teknik pemurnian. Deepublish, 2014.
15. Abriyanto, Adityas Elvian, Sabikis Sabikis, and Sudarso Sudarso. "Aktivitas anti-fungi ekstrak etanol daun sembukan (Paederia foetida L) terhadap Candida albicans." PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) 2012; 9(3).
16. Utami, Evy T., Rebecca A. Kuncoro, Islami Rahma Hutami, F. T. Sari, and J. Handajani. "Efek antiinflamasi ekstrak daun sembukan (Paederia scandens) pada tikus wistar." Majalah Obat Tradisional 2011; 16(2):95-100.
17. Ryan, K. J., and C. G. Ray. "Mycoplasma. Ryan KJ, Ray CG. Sherris Medical Microbiology." (2014).
18. Sikumalay, Adriyan, Netti Suharti, and Machdawaty Masri. "Efek Antibakteri dari Rebusan Daun Sambiloto (Andrographis paniculata Nees) dan Produk Herbal Sambiloto Terhadap Staphylococcus Aureus." Jurnal Kesehatan Andalas 2016; 5(1). https://doi.org/10.25077/jka.v5i1.468
19. Senja, Rima Yulia, E. Issusilaningtyas, A. K. Nugroho, and E. P. Setyowati. "Perbandingan metode ekstraksi dan variasi pelarut terhadap rendemen dan aktivitas antioksidan ekstrak kubis ungu (brassica oleracea l. var. capitata f. rubra)." Traditional Medicine Journal 2014; 19(1):43-48
20. Sa'adah, Lailis. "Isolasi dan identifikasi senyawa tanin dari daun belimbing wuluh (averrhoa bilimbi l.)." Skripsi tidak diterbitkan. Malang: Fakultas Sains dan Teknologi-Universitas Islam Negeri (UIN) Maulana Malik Ibrahim 2010.
21. Tetti, Mukhriani. "Ekstraksi, pemisahan senyawa, dan identifikasi senyawa aktif." Jurnal Kesehatan 2014; 7(2).
22. Stout, John David. "Aspects of the microbiology and oxidation of Wicken Fen soil." Soil Biology and Biochemistry 1971; 3(1):9-25. https://doi.org/10.1016/0038-0717(71)90027-7
23. Usman, Samsidar, and Ismail Ibrahim. "Uji Aktivitas Senyawa Bioaktif Antimikroba Dari Ekstrak Daun Sembukan (Paederia Foetida L.) Pada Bakteri Staphylococcus Aureus Dengan Metode Bioautografi." Media Farmasi 2019; 13(2):42-48. https://doi.org/10.32382/mf.v13i2.881
24. Rachmad, Basuki, Wiria Saputri, A. S. Yandi, Andi Setiawan, and Mulyono Mulyono. "Isolasi dan Identifikasi Gen Resisten Ciprofloxacin pada Isolat Escherichia coli MDR Ciprofloxacin dari Penderita ISK di RSUDAM Provinsi Lampung." Jurnal Kedokteran Universitas Lampung 2017; 1(3):487-497.
25. Kepel, Lisa, and Fona Budiarso. "Uji Resistensi Bakteri Escherichia Coli Yang Diisolasi Dari Plak Gigi Terhadap Merkuri Danantibiotik Siprofloksasin." eBiomedik 3, no. 1 (2015). https://doi.org/10.35790/ebm.3.1.2015.6604
26. Azis, Tamzil, Sendry Febrizky, and Aris D. Mario. "Pengaruh jenis pelarut terhadap persen yieldalkaloiddari daun salam india (Murraya koenigii)." Jurnal Teknik Kimia 2014; 20(2).
27. Jain J, Tikare S, Mahuli A. "Antifungal activity of ginger extract on Candida albicans: An in-vitro study." Journal of Dental Sciences and Research 2011; 2(2):18-21.
28. Fadillah, Haris. "Optimasi Sabun Cair Antibakteri Ekstrak Etanol Rimpang Jahe Merah (Zingiber Officinale Rosc. Var. Rubrum) Variasi Virgin Coconut Oil (Vco) Dan Kalium Hidroksida (Koh) Menggunakan Simplex Lattice Design." Jurnal Mahasiswa Farmasi Fakultas Kedokteran UNTAN 2014; 1(1).
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Silaban H. The Effect of Various Concentrations of Ethanol Extract of the Leaves of Paederia foetida L. on the Growth of Escherichia Coli Bacteria. JDDT [Internet]. 15Nov.2021 [cited 1Dec.2021];11(6):61-7. Available from: https://jddtonline.info/index.php/jddt/article/view/5037