Evaluation on In Vitro Blood Clot Dissolving Potential of Aqueous Extract of Sida acuta Burm. F. Leaves

  • Anita Mishra Department of Biotechnology, Vidyadayini Institute of Science, Management and Technology, Bhopal M.P. India
  • Shadma Siddiqui Department of Paramedical Sciences, SAM Global University, Raisen, M.P. India
  • Shreya Tiwari Department of Biotechnology, Shoolini University, Solan, H.P. India

Abstract

Clotting of blood is the vital processes and a perplexing interaction of various mechanisms of circulatory system due of failure of which is sometimes considered as a concern within the circulatory system causing acute myocardial or cerebral infarction which might cause demise. Sida acuta burm. f (Malvaceae) is abundantly growing small perennial shrub utilized by natives for diuretic, anthelmintic, calmative and wound healing properties, and are utilized in treating disorders like blood, bile, liver, nervous, urinary diseases and rheumatism. The present study was intended to evaluate the blood clot dissolving potential of Sida acuta leaf aqueous extract in vitro. The plant material as leaves were locally collected and subjected to phytochemical extraction with distilled water. The preliminary phytochemical tests total phenolic content was estimated by Folin-Ciocalteu’s method. In vitro thrombolytic activity of 3 different concentrations of aqueous extract was estimated on goat blood clot compared to the activity of streptokinase. The aqueous extract of S. acuta leaves  are reported to be rich in alkaloids, flavonoids, tannins, terpenoids and glycosides while the total phenolic content was estimated to be 17.48 % in extract which are mostly responsible for any pharmacological activity. Compared to the thrombolytic activity of standard streptokinase which was 73 %, the aqueous leaf extract of S. acuta displayed considerable blood clot dissolving activity at concentration 10 mg/100µl, 5.0 mg/100µl, and 2.5 mg/100µl as 41 %, 34 % and 12 % respectively. This property of plant extract is promising which could be could be exploited in development of new biopharmaceutical and therapeutic agents after stringent further physiological compatibility and in vivo pharmacological studies.


Keywords: Sida acuta, phytochemical extract, thrombolytic activity, streptokinase

Keywords: Sida acuta, phytochemical extract, thrombolytic activity, streptokinase

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Author Biographies

Anita Mishra, Department of Biotechnology, Vidyadayini Institute of Science, Management and Technology, Bhopal M.P. India

Department of Biotechnology, Vidyadayini Institute of Science, Management and Technology, Bhopal M.P. India

Shadma Siddiqui, Department of Paramedical Sciences, SAM Global University, Raisen, M.P. India

Department of Paramedical Sciences, SAM Global University, Raisen, M.P. India

Shreya Tiwari, Department of Biotechnology, Shoolini University, Solan, H.P. India

Biomedical & Biopharmaceutical Division, Lenience Biotech Lab, Bhopal, M.P. India

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Mishra A, Siddiqui S, Tiwari S. Evaluation on In Vitro Blood Clot Dissolving Potential of Aqueous Extract of Sida acuta Burm. F. Leaves. JDDT [Internet]. 15Sep.2021 [cited 20Oct.2021];11(5):96-9. Available from: https://jddtonline.info/index.php/jddt/article/view/5007