Inhibitory effects of hydroethanolic extracts from three Cameroonian medicinal plants on proteins inflammation and growth of multi-resistant strains of Mycobacterium tuberculosis

Authors

  • Esther Del florence Ndedi Moni Laboratory of Microbiology, Department of Microbiology, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon;
  • Patrick Hervé Diboue Betote Laboratory of Microbiology, Department of Microbiology, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon;
  • Christelle Wayoue Kom Laboratory of Pharmacology, Centre For Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plants Studies, P.O. Box 13033, Yaoundé, Cameroon;
  • Chimène Félicite Mekoulou Benga Multidisciplinary Laboratory, Department of Galenical Pharmacy and Pharmaceutical Law, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Cameroon;
  • Armelle Deutou Tchamgoue Laboratory of Pharmacology, Centre For Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plants Studies, P.O. Box 13033, Yaoundé, Cameroon;
  • Maximilienne Ascension Nyegue Laboratory of Microbiology, Department of Microbiology, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon;

Abstract

The present work aimed to determine the phytochemical components and evaluate the in vitro anti-inflammatory and anti-mycobacterial effects of hydroethanolic extracts of Allium sativum L bulbs, Drypetes gossweileri S. MOORE stem-barks and Pentadiplandra brazzeana Baill roots against several resistant strains of Mycobacterium tuberculosis. The phytochemical screenings of extracts were carried out according the colorimetric and precipitation tests to reveal the presence of phytochemical compounds. The anti-inflammatory effects of extracts were evaluated using in vitro Bovine Serum Albumin denaturation and proteinase inhibitory action assays. The inhibitory parameters of hydro-ethanol extracts were evaluated by the microdilution method agaisnt Mycobacterium tuberculosis. The phytochemical screening of hydro-ethanol extracts revealed the presence of phenols, polyphenols, flavonoids, alkaloids, cathechic tannins, triterpens, steroids, anthocyanins and leucoanthocyanins. The anti-inflammatory activity of hydro-ethanol extracts of D. gossweileri, P. brazzeana and A. sativum have shown the inhibitory concentrations 50 (IC50) values ranging from 356.70, 183.30 and 226.30 mg/mL for BSA denaturation and 31.92, 33.62 and 56.93 mg/mL for proteinase inhibitory action respectively. The hydroethanolic extracts of D. gossweileri, P. brazzeana and A. sativum exhibited moderate and weak anti-mycobacterial activities with the minimum inhibitory concentrations (MICs) ranging from 312.5 to 2500 μg/mL. A. sativum hydro-ethanol extract has shown the highest anti-mycobacterial activity with MIC of 312.5 μg/mL against isoniazid resistant of M. tuberculosis and extremely resistant drug strain of M. tuberculosis. These results suggest that hydro-ethanol extracts of A. sativum, D. gossweileri and P. brazzeana are efficient against tuberculosis caused by multi-resistant Mycobacterium tuberculosis strains and are able to resorb the inflammation induced during infection.

Keywords: Anti-inflammatory activity, Anti-mycobacterial effect, Hydroethanolic extracts, Medicinal plants, Phytochemical screening.

Keywords:

Anti-inflammatory activity, Anti-mycobacterial effect, Hydroethanolic extracts, Medicinal plants, Phytochemical screening

DOI

https://doi.org/10.22270/jddt.v11i4-S.4930

Author Biographies

Esther Del florence Ndedi Moni, Laboratory of Microbiology, Department of Microbiology, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon;

Laboratory for Tuberculosis Research and Pharmacology, The Biotechnology Centre, Nkolbisson, P.O. Box 3851, Yaoundé, Cameroon;

Patrick Hervé Diboue Betote, Laboratory of Microbiology, Department of Microbiology, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon;

Laboratory of Pharmacology, Centre For Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plants Studies, P.O. Box 13033, Yaoundé, Cameroon;

Multidisciplinary Laboratory, Department of Galenical Pharmacy and Pharmaceutical Law, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Cameroon;

Christelle Wayoue Kom, Laboratory of Pharmacology, Centre For Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plants Studies, P.O. Box 13033, Yaoundé, Cameroon;

Laboratory of Pharmacology, Centre For Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plants Studies, P.O. Box 13033, Yaoundé, Cameroon;

Chimène Félicite Mekoulou Benga, Multidisciplinary Laboratory, Department of Galenical Pharmacy and Pharmaceutical Law, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Cameroon;

Multidisciplinary Laboratory, Department of Galenical Pharmacy and Pharmaceutical Law, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Cameroon;

Armelle Deutou Tchamgoue, Laboratory of Pharmacology, Centre For Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plants Studies, P.O. Box 13033, Yaoundé, Cameroon;

Laboratory of Pharmacology, Centre For Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plants Studies, P.O. Box 13033, Yaoundé, Cameroon;

Maximilienne Ascension Nyegue, Laboratory of Microbiology, Department of Microbiology, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon;

Multidisciplinary Laboratory, Department of Galenical Pharmacy and Pharmaceutical Law, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Cameroon;

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Published

2021-08-15
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Moni ED florence N, Betote PHD, Kom CW, Benga CFM, Tchamgoue AD, Nyegue MA. Inhibitory effects of hydroethanolic extracts from three Cameroonian medicinal plants on proteins inflammation and growth of multi-resistant strains of Mycobacterium tuberculosis. J. Drug Delivery Ther. [Internet]. 2021 Aug. 15 [cited 2026 Jan. 22];11(4-S):15-21. Available from: https://jddtonline.info/index.php/jddt/article/view/4930

Issue

Vol. 11 No. 4-S (2021): Volume 11, Issue 4-S, July-August 2021

Section

Research

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How to Cite

1.
Moni ED florence N, Betote PHD, Kom CW, Benga CFM, Tchamgoue AD, Nyegue MA. Inhibitory effects of hydroethanolic extracts from three Cameroonian medicinal plants on proteins inflammation and growth of multi-resistant strains of Mycobacterium tuberculosis. J. Drug Delivery Ther. [Internet]. 2021 Aug. 15 [cited 2026 Jan. 22];11(4-S):15-21. Available from: https://jddtonline.info/index.php/jddt/article/view/4930
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