Development and Validation of TLC of Flavonoid from the Ethanolic Extract of Plant Enhydra fluctuans
Abstract
Thin layer chromatography is a technique or an analytical tool to separate the bioactive compound from the mixture of components. In current research work special attention was given to develop specific solvent system and to validate the principle of separation of Flavonoid. The ethanolic extract of plant namely Helencha (in Bengali) was selected for such purpose. After several trials, the presence of Flavonoid which was confirmed by qualitative evaluation and was sepahjvvrated successfully under this study and the process was validated under the circumstances of ICH Guideline. The plant not only contained Flavonoid but there were the presence of little quantity of Alkaloid, Saponin and Tannins also. Due to presence of Flavonoid the ethanolic fraction of the plant may be evaluated for Anti-inflammatory and Anthelmintic activity for further research. The plant Helencha is known as Enhydra fluctuans belongs to the family Astereceae. According to folklore claim the plant is useful for nutrition purpose. Not only that the plant is also useful in Dropsy, anasarca and snake bite. As per the literature survey, the plant has Antioxidant and Analgesic activity. Here the total attention was given to separate Flavonoid from the mixture of Components present in the ethanolic fraction of the leaves of the plant.
Keywords: Flavonoid, Alkaloid, ICH Guideline, Enhydra fluctuans, Dropsy, Anaasrca.
Keywords:
Flavonoid, Alkaloid, ICH Guideline, Enhydra fluctuans, Dropsy, AnaasrcaDOI
https://doi.org/10.22270/jddt.v11i4-S.4915References
Madic SM, Males Z, Saric S, Debeljak Z, Liq J; Chromatogr. Technol. 1999; 22:82-103.
Jork H, Funk W, Fischer W, Wimmer H. Thin-Layer Chromatography Reagents and Detection Methods: vol 1: VCH Verlagsgesellschaftmb H, D-6940 Weinheim (Federal Republic of Germany), 1990 .p. 79-82
Chemistry chromatography, 2009 [cited 2009 sep 9]: Available from:http://www.marz-kreations.com/Chemistry/Chromatography/Dyes/RF-Values.html
Salunke PA, Barhate SD, Chavhan BR, Patil SS, Wagh BR, Rathod BR; Separation of Dyes by Mixed Hydrotropic Thin Layer Chromatography. Asian Journal of Pharmaceutical Analysis, 2019: DOI: https://doi.org/10.5958/2231-5675.2019.00027.9
Momin MAM, Khan RM, Rayhan J, Afrose A, Rana S, Begum AA;A. Ghani, Medicinal Plants of Bangladesh: Chemical Constituents & Uses,2nd Edition, Asiatic Society of Bangladesh, Dhaka, 2003 .p. 1-16.
Satyajit, Pradhan D, Natural Flavonoids Isolated from leaves of Enhydafluctuans inhibit cyclooxygenase-2 and 5, 2012; 2(1):65-71.
Hazra, H, Alfasane, MA, Kauser S, Shahjadee UF, Khondker M; Biochemical Composition of Some Selected Aquatic Macrophytes under Ex-Situ Conditions. Journal of the Asiatic Society of Bangladesh, Science, 2018; 44(1):53-60 https://doi.org/10.3329/jasbs.v44i1.46545
Markham KR, Techniques of Flavonoids Identification, Academic Press, London 1982.p. 33-39
Wagnar H, Bladt S; Plant Drug Analysis, 2nd ed. Berlin: springer dordrechtheidelberglondon new York; 1996 .p. 195. https://doi.org/10.1007/978-3-642-00574-9
Feng W, Hao Z, Li M; Isolation and structure Identification of Flavonoids, China: 2017; DOI: https://doi.org/10.5772/67810
Mukherjee KP. Evaluating natural products and traditional medicine. 1st ed.Imprint: Elsevier; 2019; p.360-402
ICH Harmonised Tripartite Guideline: Validation of Analytical Procedures: Text and Methodology, Q2 (R1), ICH, Geneva, Switzerland; 2005. Available from: http://www.ich.org.
Nair CVJ, Ahamad S, Khan W, Anjum V, Mathur R; Development and validation of High-performance Thin-layer Chromatography Method for Simultaneous Determination of Polyphenolic Compounds in Medicinal Plants. Pharmacognosy Research, 2017; 9(1):67-73 https://doi.org/10.4103/pr.pr_122_16
Banavatu L; TLC slide share 2015[cited 2015 Jan 7]: Available from: https://www.slideshare.net/LavakusaBanavatu/thin-layer-chromatography-43293607
Bereznitski, Yuri, Thompson, Richard, Neill O, Eavan, Grinberg, Nelu. "Thin Layer Chromatography-A Useful Technique for the Separation of Enantiomers." Journal of AOAC International, 2001; 84:1242-1251.
Tavallali H, Zareiyan FS, Naghian M, "An efficient and simultaneous analysis of caffeine and paracetamol in pharmaceutical formulations using TLC with a fluorescence plate reader," Journal of AOAC International, 2011; 94(4):1094-1099. https://doi.org/10.1093/jaoac/94.4.1094
Seikel MK, Geissman; In The Chemistry of Flavonoid Compounds, Pergamon Press, New York, 1962; 34.
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