Investigation of Effect of Nanosecond Pulsed Electric Field on MCF-7 Breast Cancer Cells

  • GYANENDRA KUMAR HOMI BHABHA NATIONAL INSTITUTE MUMBAI https://orcid.org/0000-0002-5246-4486
  • Sandeep Shelar Homi Bhabha National Institute, Mumbai-400085, India
  • Ankur Patel Accelerator and Pulse Power Division, Bhabha Atomic Research Centre, Mumbai-400085
  • Amitava Roy Accelerator and Pulse Power Division, Bhabha Atomic Research Centre, Mumbai-400085
  • Ramanujam Sarathi Department of Electrical Engineering, Indian Institute of Technology Madras, Chennai, India
  • Rajshri Singh Amity Institute of Biotechnology, Amity University Mumbai, Maharashtra-410206
  • Archana Sharma Accelerator and Pulse Power Division, Bhabha Atomic Research Centre, Mumbai-400085

Abstract

Pulsed electric field therapy is a novel non-invasive approach for cancer therapy. It serves as a cell permeability enhancing agent for cancer treatment. Nanosecond, high-electrical field pulse power technology is used for delivering variable, controllable, intracellular electrical perturbations in several biological systems. Here, we investigated the effect of nanosecond (ns) electric pulse (nsEP) as a therapeutic tool for cancer. In in-vitro study, the breast cancer cells (MCF-7) were exposed with electric field of ~18kV/cm intensity, ~25ns duration, at 1.5Hz in a 2mm electroporation cuvette. Post exposure, observation shows a significant reduction in cell viability. It was evident that after treatment the viability of MCF-7 cancer cells at 630 pulses are remains ~38% only. The optical microscopic analysis of MCF-7 cells shows cell morphology changes after electrical pulse exposure. Moreover, we have also investigated a comparative study of the effect nano-second electrical pulses on MCF-7 cells and Chinese Hamster Ovary (CHO) cell line. The comparative study, demonstrated that the effect of nsEPF on MCF-7 is more destructive than on CHO cell line. The obtained results support that the pulse electrical field of nanosecond (ns) duration therapy would be a potential solution for cancer treatment.


Keywords: Nanosecond Pulse Electric Field; Full Width at Half Maxima; Pulse Forming Line; Pulse Exposure; Viability;

Keywords: Nanosecond Pulse Electric Field, Full Width at Half Maxima, Pulse Forming Line, Pulse Exposure, Viability

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Author Biographies

GYANENDRA KUMAR, HOMI BHABHA NATIONAL INSTITUTE MUMBAI

Homi Bhabha National Institute, Mumbai-400085, India

Sandeep Shelar, Homi Bhabha National Institute, Mumbai-400085, India

Homi Bhabha National Institute, Mumbai-400085, India

Ankur Patel, Accelerator and Pulse Power Division, Bhabha Atomic Research Centre, Mumbai-400085

Homi Bhabha National Institute, Mumbai-400085, India

Amitava Roy, Accelerator and Pulse Power Division, Bhabha Atomic Research Centre, Mumbai-400085

Homi Bhabha National Institute, Mumbai-400085, India

Ramanujam Sarathi, Department of Electrical Engineering, Indian Institute of Technology Madras, Chennai, India

Homi Bhabha National Institute, Mumbai-400085, India

Rajshri Singh, Amity Institute of Biotechnology, Amity University Mumbai, Maharashtra-410206

Homi Bhabha National Institute, Mumbai-400085, India

Archana Sharma, Accelerator and Pulse Power Division, Bhabha Atomic Research Centre, Mumbai-400085

Homi Bhabha National Institute, Mumbai-400085, India

References

1. Ferraro B, Cruz YL, Coppola D, Heller R. Intradermal delivery of plasmid VEGF (165) by electroporation promotes wound healing. Mol Ther 2009; 17(4):651-7.
2. Heller L, Jaroszeski MJ, Coppola D, Pottinger C, Gilbert R, Heller R. Electrically mediated plasmid DNA delivery to hepatocellular carcinomas in vivo. Gene Ther 2000; 7(10):826-9.
3. Bodles-Brakhop AM, Heller R, Draghia-Akli R. Electroporation for the delivery of DNA-based vaccines and immunotherapeutics: current clinical developments. Mol Ther 2009; 17(4):585-92.
4. Livingston BD, Little SF, Luxembourg A, Ellefsen B, Hannaman D. Comparative performance of a licensed anthrax vaccine versus electroporation-based delivery of a PA encoding DNA vaccine in rhesus macaques. Vaccine 2010; 28(4):1056-61.
5. Donate A, Coppola D, Cruz Y, Heller R. Evaluation of a novel non-penetrating electrode for use in DNA vaccination. PLoS One 2011; 6(4):0019181.
6. Okino M, Mohri H. Effects of a high-voltage electrical impulse and an anticancer drug on in vivo growing tumors. Jpn J Cancer Res 1987; 78(12):1319-21.
7. Orlowski S, Belehradek J, Jr., Paoletti C, Mir LM. Transient electropermeabilization of cells in culture. Increase of the cytotoxicity of anticancer drugs. Biochem Pharmacol 1988; 37(24):4727-33.
8. Heller R, Jaroszeski MJ, Glass LF, Messina JL, Rapaport DP, DeConti RC, et al. Phase I/II trial for the treatment of cutaneous and subcutaneous tumors using electrochemotherapy. Cancer 1996; 77(5):964-71.
9. Gehl J, Skovsgaard T, Mir LM. Enhancement of cytotoxicity by electropermeabilization: an improved method for screening drugs. Anticancer Drugs 1998; 9(4):319-25.
10. Heller R, Coppola D, Pottinger C, Gilbert R, Jaroszeski MJ. Effect of electrochemotherapy on muscle and skin. Technol Cancer Res Treat 2002; 1(5):385-92.
11. Gothelf A, Mir LM, Gehl J. Electrochemotherapy: results of cancer treatment using enhanced delivery of bleomycin by electroporation. Cancer Treat Rev 2003; 29(5):371-87.
12. Munoz Madero V, Ortega Perez G. Electrochemotherapy for treatment of skin and soft tissue tumours. Update and definition of its role in multimodal therapy. Clin Transl Oncol 2011; 13(1):18-24.
13. Testori A, Tosti G, Martinoli C, Spadola G, Cataldo F, Verrecchia F, et al. Electrochemotherapy for cutaneous and subcutaneous tumor lesions: a novel therapeutic approach. Dermatol Ther 2010; 23(6):651-61.
14. Yang XJ, Li J, Sun CX, Zheng FY, Hu LN. The effect of high frequency steep pulsed electric fields on in vitro and in vivo antitumor efficiency of ovarian cancer cell line skov3 and potential use in electrochemotherapy. J Exp Clin Cancer Res 2009; 28(53):1756-9966.
15. Kirson ED, Dbaly V, Tovarys F, Vymazal J, Soustiel JF, Itzhaki A, et al. Alternating electric fields arrest cell proliferation in animal tumor models and human brain tumors. Proc Natl Acad Sci U S A 2007; 104(24):10152-7.
16. Persson BR, Baureus Koch C, Grafstrom G, Engstrom PE, Salford LG. A model for evaluating therapeutic response of combined cancer treatment modalities: applied to treatment of subcutaneously implanted brain tumors (N32 and N29) in Fischer rats with pulsed electric fields (PEF) and 60Co-gamma radiation (RT). Technol Cancer Res Treat 2003; 2(5):459-70.
17. Kubota Y, Nakada T, Sasagawa I. Treatment of rat bladder cancer with electrochemotherapy in vivo. Methods Mol Med 2000;37: 293-8.
18 Hofmann GA, Dev SB, Dimmer S, Nanda GS. Electroporation therapy: a new approach for the treatment of head and neck cancer. IEEE Trans Biomed Eng 1999; 46(6):752-9.
19. Al-Sakere B, André F, Bernat C, Connault E, Opolon P, Davalos RV, et al. Tumor ablation with irreversible electroporation. PLoS One 2007; 2(11): e1135-e.
20. Davalos RV, Mir IL, Rubinsky B. Tissue ablation with irreversible electroporation. Ann Biomed Eng 2005; 33(2):223-31.
21. Onik G, Mikus P, Rubinsky B. Irreversible electroporation: implications for prostate ablation. Technol Cancer Res Treat 2007; 6(4):295-300.
22. Rubinsky B. Irreversible electroporation in medicine. Technol Cancer Res Treat 2007; 6(4):255-60.
23. Rubinsky B, Onik G, Mikus P. Irreversible electroporation: a new ablation modality-clinical implication. Technol Cancer Res Treat 2007; 6(1):37-48.
24. Lee EW, Loh CT, Kee ST. Imaging guided percutaneous irreversible electroporation: ultrasound and immunohistological correlation. Technol Cancer Res Treat 2007; 6(4):287-94.
25. Garcia PA, Rossmeisl JH, Jr., Robertson J, Ellis TL, Davalos RV. Pilot study of irreversible electroporation for intracranial surgery. Conf Proc IEEE Eng Med Biol Soc 2009; 6(10):5333141.
26. Vollherbst D, Fritz S, Zelzer S, Wachter MF, Wolf MB, Stampfl U, et al. Specific CT 3D rendering of the treatment zone after Irreversible Electroporation (IRE) in a pig liver model: the "Chebyshev Center Concept" to define the maximum treatable tumor size. BMC Med Imaging 2014; 14:2-.
27. Stampflj R. Reversible electrical breakdown of the excitable membrane of a Ranvier node. Anais da Academia Brasileira de Ciencias 1958; 30(1):57-61.
28. Neumann E, Rosenheck K. Permeability changes induced by electric impulses in vesicular membranes. The Journal of Membrane Biology 1972; 10(1):279-90.
29. Schoenbach KH, Hargrave SJ, Joshi RP, Kolb JF, Nuccitelli R, Osgood C, et al. Bioelectric Effects of Intense Nanosecond Pulses. IEEE Transactions on Dielectrics and Electrical Insulation 2007; 14(5):1088-109.
30. Nuccitelli R, McDaniel A, Anand S, Cha J, Mallon Z, Berridge JC, et al. Nano-Pulse Stimulation is a physical modality that can trigger immunogenic tumor cell death. J Immunother Cancer 2017; 5(32):017-0234.
31. Cole KS. Electric Impedance of Marine Egg Membranes. Nature 1938; 141(3558):79-.
32. Schoenbach K, Beebe S, Buescher E. Intracellular effect of ultrashort pulses. Bioelectromagnetics 2001; 22: 440-8.
33. Schoenbachk KH, Joshi RP, Kolb JF, Chen N, Stacey M, Buescher ES, et al. Ultrashort electrical pulses open a new gateway into biological cells. Conference Record of the Twenty-Sixth International Power Modulator Symposium, 2004 and 2004 High-Voltage Workshop.; 2004:205-9.
34. Buescher ES, Schoenbach KH. Effects of submicrosecond, high intensity pulsed electric fields on living cells - intracellular electromanipulation. IEEE Transactions on Dielectrics and Electrical Insulation 2003; 10(5):788-94.
35. Zhang H, Liu K, Xue Z, Yin H, Dong H, Jin W, et al. High-voltage pulsed electric field plus photodynamic therapy kills breast cancer cells by triggering apoptosis. American journal of translational research 2018; 10(2):334-51.
36. Wu S, Guo J, Wei W, Zhang J, Fang J, Beebe SJ. Enhanced breast cancer therapy with nsPEFs and low concentrations of gemcitabine. Cancer Cell Int 2014; 14(1):014-0098.
37. Sundararajan R, Xiao F, Salameh T, Reece LM, Leary JF, Otto K, et al. Effective proliferation control of human cancer cells using electrical pulses. IEEE Transactions on Dielectrics and Electrical Insulation 2012; 19(6):2225-36.
38. Rezaee, Z., Yadollahpour, A., & Bayati, V. Single Intense Microsecond Electric Pulse Induces Radiosensitization to Ionizing Radiation: Effects of Time Intervals Between Electric Pulse and Ionizing Irradiation. Frontiers in oncology, 2018; 8:418. https://doi.org/10.3389/fonc.2018.00418
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1.
KUMAR G, Shelar S, Patel A, Roy A, Sarathi R, Singh R, Sharma A. Investigation of Effect of Nanosecond Pulsed Electric Field on MCF-7 Breast Cancer Cells. JDDT [Internet]. 15May2021 [cited 18Sep.2021];11(3):43-9. Available from: https://jddtonline.info/index.php/jddt/article/view/4827