Effect of Arabica Coffee Bean Extract (Coffea arabica) as a Growth Inhibitor of Enterococcus faecalis ATCC 29212
Abstract
Arabica coffee seeds contain a composition of Caffeine, Chlorogenic acid, Flavonoids, and Trigonelline. The purpose of this study is to test the antibacterial of Arabica coffee seed extract against bacterium Enterococcus faecalis at concentrations of 1,5625%, 3,125%, 6,25%, 12,5%, 25%, 50% and 100%. The extraction method using maceration with solvent 96%. Antibacterial activity test was done by using the Kirby and Bauer diffusion test method. The results showed that Arabica coffee seed extract could provide inhibition starting from 3,125% with an average diameter of inhibition zone of 1,16 mm to the largest concentration of 100% with an average zone diameter of inhibition of 14,6 mm. At the same time, the average diameter of the inhibitory zone of antibiotic ampicillin at a concentration of 1% as a control (+) is 24,6 mm. The results showed that the greater concentration, the greater the inhibitory zones are formed.
Keywords: Coffea arabica, Enterococcus faecalis, antibacterial, inhibition zone
Keywords:
Coffea arabica, Enterococcus faecalis, antibacterial, inhibition zoneDOI
https://doi.org/10.22270/jddt.v11i3.4820References
Imran, H. Effect of Coffee Consumption on Decreasing PH of Silvida in Adults. Ponorogo Health Scientific Forum. 2019.
Chismirina S, Andayani R, Ginting R. Pengaruh Kopi Arabika (Coffea Arabica) Dan Kopi Robusta (Coffea Canephora) Terhadap Viskositas Saliva Secara In Vitro. Cakradonya Dental Journal. 2014; 6(2):687-91.
Nurmilawati MA. The Effect of Robusta Coffe Extract (Coffea Robusta) as Inhibitors of Growth Staphylococcus Aureus. InSeminar Nasional XII Pendidikan Biologi FKIP UNS 2015 2015. Sebelas Maret University.
Harseno S, Mooduto L, Prasetyo EP. DAYA ANTIBAKTERI EKSTRAK DAUN KEDONDONG BANGKOK (Spondias dulcis Forst.) TERHADAP BAKTERI Enterococcus faecalis Antibacterial Potency of Kedondong Bangkok Leaves Extract (Spondias dulcis Forst.) against Enterococcus faecalis Bacteria. Conservative Dentistry Journal. 2016 Jul 31; 6(2):52-8. https://doi.org/10.20473/cdj.v6i2.2016.110-116
Nurdin D, Hardiansyah A, Chaldun ER, Fikkriyah AK, Dharsono HD, Kurnia D, Satari MH. Preparation and Characterization of Terpenoid-Encapsulated PLGA Microparticles and its Antibacterial Activity against Enterococcus faecalis. InKey Engineering Materials 2020 (Vol. 829, pp. 263-269). Trans Tech Publications Ltd. https://doi.org/10.4028/www.scientific.net/KEM.829.263
Lissanawidya P, Risandiansyah R, Airlangga H. Frequency of resistance to Enterococcus faecalis against Roselle decoction (Hibiscus sabdariffa Linn) and Amoxicillin antibiotics. JIMR-Journal of Islamic Medicine Research. 2017 Jun 6; 1(1).
Hiwot H. Growth and Physiological Response of Two Coffea Arabica L. Population under Higha and Low Irradiance (Doctoral dissertation, Thesis. Addis Ababa University).
Mulyani A, Las I. Land resources potential and optimalization of bioenergy producing commodities development in Indonesia. Jurnal Penelitian dan Pengembangan Pertanian. 2008; 27(1):31-41.
Gumulya D, Helmi IS. Study of Indonesian coffee drinking culture. Jurnal Dimensi Seni Rupa dan Desain. 2017 Feb 1; 13(2):153-72. https://doi.org/10.25105/dim.v13i2.1785
Fajriana NH, Fajriati I. Analysis of Arabica Coffee (Coffea arabica L.) Caffeine Levels at Variations in Roasting Temperature by Ultra Violet Spectrophotometry. Analit: Analytical and Environmental Chemistry. 2018 Oct 30; 3(2). https://doi.org/10.23960/aec.v3.i2.2018.p148-162
Farhaty N, Muchtaridi M. Tinjauan kimia dan aspek farmakologi senyawa asam klorogenat pada biji kopi. Farmaka. 2016; 14(1):214-27.
Lou Z, Wang H, Zhu S, Ma C, Wang Z. Antibacterial activity and mechanism of action of chlorogenic acid. Journal of food science. 2011 Aug; 76(6):M398-403. https://doi.org/10.1111/j.1750-3841.2011.02213.x
Xie Y, Yang W, Tang F, Chen X, Ren L. Antibacterial activities of flavonoids: structure-activity relationship and mechanism. Current medicinal chemistry. 2015 Jan 1; 22(1):132-49. https://doi.org/10.2174/0929867321666140916113443
Almeida AA, Farah A, Silva DA, Nunan EA, Glória MB. Antibacterial activity of coffee extracts and selected coffee chemical compounds against enterobacteria. Journal of agricultural and food chemistry. 2006 Nov 15; 54(23):8738-43. https://doi.org/10.1021/jf0617317
Tanauma HA. Aktivitas antibakteri ekstrak biji kopi robusta (Coffea canephora) terhadap bakteri Escherichia coli. Pharmacon. 2016 Oct 31; 5(4).
Getachew AT, Chun BS. Influence of hydrothermal process on bioactive compounds extraction from green coffee bean. Innovative food science & emerging technologies. 2016 Dec 1; 38:24-31. https://doi.org/10.1016/j.ifset.2016.09.006
Suchitra U, Kundabala M. Enterococcus Faecalis-An Endodontic Pathogen. Endodontology. 2006; 18(2):11-3.
Kayaoglu G, Ørstavik D. Virulence factors of Enterococcus faecalis: relationship to endodontic disease. Critical Reviews in Oral Biology & Medicine. 2004 Sep; 15(5):308-20. https://doi.org/10.1177/154411130401500506
Vos P, Garrity G, Jones D, Krieg NR, Ludwig W, Rainey FA, Schleifer KH, Whitman WB, editors. Bergey's manual of systematic bacteriology: Volume 3: The Firmicutes. Springer Science & Business Media; 2011 Jan 28.
Portenier I, Waltimo TM, Haapasalo M. Enterococcus faecalis-the root canal survivor and 'star'in post‐treatment disease. Endodontic topics. 2003 Nov; 6(1):135-59. https://doi.org/10.1111/j.1601-1546.2003.00040.x
Sedgley CM, Lennan SL, Clewell DB. Prevalence, phenotype and genotype of oral enterococci. Oral microbiology and immunology. 2004 Apr; 19(2):95-101. https://doi.org/10.1111/j.0902-0055.2004.00122.x
Nonthakaew A, Matan N, Aewsiri T, Matan N. Caffeine in foods and its antimicrobial activity. International Food Research Journal. 2015 Jan 1; 22(1).
Ezeh O, Gordon MH, Niranjan K. Enhancing the recovery of tiger nut (Cyperus esculentus) oil by mechanical pressing: Moisture content, particle size, high pressure and enzymatic pre-treatment effects. Food chemistry. 2016 Mar 1; 194:354-61. https://doi.org/10.1016/j.foodchem.2015.07.151
Purbaya S, Aisyah LS, Nopitasari D. The Effectiveness of Adding Red Fruit Oil (Pandanus conoideus Lamk.) into Ethanol Extract of Temulawak rhizome (Curcuma xanthorrhiza Roxb.) as Antioxidant. Jurnal Kimia Sains dan Aplikasi. 2020; 23(11):409-13. https://doi.org/10.14710/jksa.23.11.409-413
Owczarek K, Szczepanska N, Plotka-Wasylka J, Rutkowska M, Shyshchak O, Bratychak M, Namiesnik J. Natural deep eutectic solvents in extraction process. Chemistry & Chemical Technology. 2016; 10(4):601-6. https://doi.org/10.23939/chcht10.04si.601
Aziz T, KN RC, Fresca A. Pengaruh pelarut heksana dan etanol, volume pelarut, dan waktu ekstraksi terhadap hasil ekstraksi minyak kopi. Jurnal Teknik Kimia. 2009 Jan 1; 16(1).
Viera VB, Piovesan N, Rodrigues JB, de O Mello R, Prestes RC, dos Santos RC, de A Vaucher R, Hautrive TP, Kubota EH. Extraction of phenolic compounds and evaluation of the antioxidant and antimicrobial capacity of red onion skin (Allium cepa L.). International Food Research Journal. 2017 Jul 1; 24(3).
Maulyanti R. Activity Test of Cempedak (Arthocarpus champeden) Leaf Ethanol Extract Gel Against Acne-causing Bacteria (Doctoral dissertation, Universitas Islam Negeri Alauddin Makassar).
Ballesteros LF, Teixeira JA, Mussatto SI. Selection of the solvent and extraction conditions for maximum recovery of antioxidant phenolic compounds from coffee silverskin. Food and bioprocess technology. 2014 May; 7(5):1322-32. https://doi.org/10.1007/s11947-013-1115-7
Chen J, Nan G, Yang R, Zhang S, Bian X, Yang G. Solubility of three types of benzamide derivatives in toluene, ethyl acetate, acetone, chloroform, methanol, and water at temperatures ranging from 288.15 to 328.15 K at atmospheric pressure. Journal of Molecular Liquids. 2015 Apr 1; 204:137-46. https://doi.org/10.1016/j.molliq.2015.01.045
Wijaya W, Ridwan RD, Budi HS. The antibacterial ability of arabica (Coffea arabica) and robusta (Coffee Canephora) Coffee Extract on Lactobacillus acidophillus. Dental Jurnal (Majalah Kedokteran Gigi). 2016; 49(2):99-103. https://doi.org/10.20473/j.djmkg.v49.i2.p99-103
Milah N. The Effect of Propolis Antibacterial Concentration on Bacterial Growth Streptococcus pyogenes Secara In Vitro (Doctoral dissertation, Universitas Negeri Semarang).
Aljufri, A. H. (2010). The Effect of CMC-Na Concentration Variations as a Binder on Physical Properties and Inhibition of Streptococcus Mutans Bacteria in Black Tea Extract Toothpaste Preparations (Camellia sinensis) (Doctoral dissertation, Universitas Muhammadiyah Surakarta).
Aljufri, A. H. (2010). The Effect of CMC-Na Concentration Variations as a Binder on Physical Properties and Inhibition of Streptococcus Mutans Bacteria in Black Tea Extract Toothpaste Preparations (Camellia sinensis) (Doctoral dissertation, Universitas Muhammadiyah Surakarta).
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