The Effectiveness of Giving Natural Honey as a Bactericide Against the Growth of Escherichia coli Bacteria

  • Mulyadi Djojosaputro Faculty of Medicine, Universitas Kristen Indonesia, Jakarta, Indonesia
  • Moskwadina Gultom Faculty of Medicine, Universitas Kristen Indonesia, Jakarta, Indonesia


Honey is one of the natural treatments with antibacterial effects such as hydrogen peroxide, low pH and short water activity that may inhibit bacterial growth. This study aimed to determine the activity of honey as antibacterial to bacteria Escherichia coli. Antibacterial activity test was done in vitro through diffusion method by using disk disc in MHA media then measuring the diameter of a clear zone that inhibition zone form growth of Escherichia coli bacteria and scaled it millimetre unit. This research was done in the laboratory of the Faculty of Medicine Universitas Kristen Indonesia. The concentrations used are 10%, 30%, 50%, 70%, 100% by using sterile equates as honey diluents. The control used was chloramphenicol, the results obtained at a concentration of 10% with a zone of 7.6 mm inhibition of honey had been able to inhibit the growth of Escherichia coli bacteria, and the optimum concentration was 100% with a zone of 24.5 mm. This test showed that natural honey could inhibit bacteria, which conclude that honey might be used as alternative medicine in society.

Keywords: Antibacterial effect, Escherichia coli, honey

Keywords: Antibacterial effect, Escherichia coli, honey


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Author Biographies

Mulyadi Djojosaputro, Faculty of Medicine, Universitas Kristen Indonesia, Jakarta, Indonesia

Faculty of Medicine, Universitas Kristen Indonesia, Jakarta, Indonesia

Moskwadina Gultom, Faculty of Medicine, Universitas Kristen Indonesia, Jakarta, Indonesia

Faculty of Medicine, Universitas Kristen Indonesia, Jakarta, Indonesia


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Djojosaputro M, Gultom M. The Effectiveness of Giving Natural Honey as a Bactericide Against the Growth of Escherichia coli Bacteria. JDDT [Internet]. 30May2021 [cited 28Sep.2021];11(3):183-7. Available from: