Development and Evaluation of Nutritious Supplementary Food Product in Phenyl Hydrazine Induced Anaemia in Wistar Rats
Objective- Nutritional deficiency anemia mainly results from a lack of iron, but it also associated due to lack of folate/vitamin B12 and low vitamin C intake. From various studies this is found anemia in people is due to poor eating habits, such as not eating enough fruits and legumes such as beans and peas. Proper nutritional supplementation may be useful in such condition. The aim of the present work was to select a suitable iron rich food material, and prepared a suitable dosage form for management of anaemic condition and to evaluate anti-anaemic potential of nutritious supplementary food. Method- Anemia was induced by phenyl-hydrazine. Animals were treated with nutritious supplementary food throughout the study for 30 days. The haemoglobin concentration was determined after 30 days. Results- Nutritious supplementary food was found to be rich in folic acid, ascorbic acid, and iron. Following the induction of anaemia, the haemoglobin concentration decreased by 30%. Administration of standard hematinic preparation and nutritious supplementary food 2gm/day in divided dose resulted in significant increase (P ≤ 0.05) in haemoglobin concentration when compared to the untreated phenyl hydrazine-induced anaemic rats. Conclusion- Nutritious supplementary food effectively raised the level of haemoglobin. Vitamin and minerals found in nutritious supplementary food are most likely active ingredients responsible for its hematinic effects.
Keywords- Anaemia, nutrition, nutritional anaemia, iron, phenyl hydrazine
2. Rebecca J. Stoltzfus, Luke Mullany and Robert E. Black, Iron deficiency anaemia, p. 64-204.
3. Singh S, Parihar S, “Prevalence of anemia in under five-year-old children” a hospital-based study, International Journal of Contemporary Pediatrics, 2019 Mar; 6(2):842-847, DOI: http://dx.doi.org/10.18203/2349-3291.ijcp20190740
4. International Institute for Population Sciences, Family Health Survey-2. Anemia among women and children. Mumbai: IIPS, 2000.
5. Michaelsen KF, Weaver L, Francesco Branca and Aileen Robertson, “Feeding and nutrition of infants and young children” ISBN 9289013540, ISSN 0378-2255.
6. World Health Organization 2017 Nutritional anaemia: "tools for effective prevention and control ISBN 978-92-4-151306-7.
7. Brian Thompson, Food-based approaches for combating iron deficiency, p.2-21.
8. Ekta K. Kalra (2003) Nutraceutical - Definition and Introduction. AAPS PharmSci 2003; 5(3):25 (http://www.pharmsci.org).
9. Duke, James A. 1992. Handbook of phytochemical constituents of GRAS herbs and other economic plants.
10. Gaud RS, Gupta GD, Practical Pharmaceutics, CBS Publication and distributors Pvt. Ltd. 2016, p. 164-167.
11. Jaiswal A, Ganeshpurkar A, Awasthi A, Bansal D, Dubey N, Protective Effects of Beetroot Extract against Phenyl Hydrazine Induced Anemia in Rats, PHCOG J, Sep-Oct 2014; 5:1-3.
12. Singh J, Asava & Arishta, Lohasav, Aug 16, 2016, https://www.ayurtimes.com/lohasava-lohasavam.
13. Tawfique M, Jamal CY, “Approach to Diagnosis of Anemia in Children”, Northern International Medical College Journal, DOI: http://dx.doi.org/10.3329/nimcj.v9i1.35920, Northern International Medical College Journal 2017; 9(1):248-251.
14. World Health Organization 2016, “Guideline: Daily iron supplementation in adult women and adolescent girls”, ISBN 978 92 4 151019 6.
15. World Health Organization 2016, “Guideline: daily iron supplementation in infants and children”, ISBN 978 92 4 154952 3 .
16. Deaton A, Drèze J, Food and Nutrition in India: Facts and Interpretations, february 14, 2009 vol xliv No 7.
17. Zimmermann MB, Hurrell, RF“Nutritional iron deficiency”, Lancet 2007; 370: 511–20.
18. Health supplements and nutraceuticals– Emerging high growth sector in India, world food India 2017.
19. ASA & Associate LLP, A brief report on nutraceutical product in India, p. 2-7.
20. Broek, NVD Anaemia and micronutrient deficiencies, British Medical Bulletin 2003; 67: 149–160 DOI: 10.1093/bmb/ldg004.
21. World Health Organization 2015, The global prevalence of anaemia in 2011, ISBN 978 92 4 156496 0.
22. Bhardwaj AK, Raina SK, Rapid Assessment for Coexistence of Vitamin B12 and Iron Deficiency Anemia among Adolescent Males and Females in Northern Himalayan State of India, Volume 2013, Article ID 959605, http://dx.doi.org/10.1155/2013/959605, pp.2-5.
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