Isolation and Characterization of Bioactive Components Derived from Whole Plant of Selaginella bryopteris
Abstract
Natural products and herbal remedies used in traditional folklore medicine have been the source of many medically beneficial drugs because they elicit fewer side effects, relatively cheap, affordable and claimed to be effective. However, in order to make these remedies acceptable to modern medicine, there is a need to scientifically evaluate them to identify the active principles and to understand their mechanism of action. Selaginella bryopteris (S. bryopteris) is a pteridophytic plant belongs to the family selaginellaceae. Its familiar name is sanjeevani booti. The aim of the present investigation was isolation and characterization of active components derived from whole plant of S. bryopteris. The plant was extracted with petroleum ether, ethyl acetate and methanol solvent. The preliminary phytochemical results revealed that alkaloids, carbohydrates, reducing sugar, flavonoids, protein, amino acid, tannin and phenolic compound as active constituents in methanolic extract of S. bryopteris. The total phenolics content of whole plant of methanolic extract was (184.13±0.416 mg/gm), followed by flavonoids (89.67±2.516mg/gm). The quantification and the identification of compounds in the crude extract and active bands isolated by preparative TLC were accomplished using spectroscopic analysis. The most important compounds amentoflavone identified in the crude extract appreciable amounts may account for its various biological activities.
Keywords: Selaginella bryopteris, Isolation, Plant extraction, Phytochemical
Keywords:
Selaginella bryopteris, Isolation, Plant extraction, PhytochemicalDOI
https://doi.org/10.22270/jddt.v10i4-s.4255References
Rahmani AH, Aly SM, Ali H, Babiker AY, Srikar S, Khan AA. Therapeutic effects of date fruits (Phoenix dactylifera) in the prevention of diseases via modulation of anti-inflammatory, antioxidant and anti-tumour activity. Int J Clin Exp Med. 2014; 7:483-491.
Rahmani AH, Aly SM. Nigella sativa and its active constituent’s thymoquinone shows pivotal role in the diseases prevention and treatment. Asian J Pharm Clin Res. 2015; 8:48-53.
Shukla AN, Srivastava S & Rawat AKS. An ethnobotanical study of medicinal plants of Rewa district, Madhya Pradesh. Ind J Trad Knowl. 2010; 9:191-202.
Singh S, Dixit RD, Sahu TR. Ethnomedicinal uses of pteridophytes of Amarkantak, Madhya Pradesh. Ind J Trad Knowl. 2010; 4:392–395.
Silva GL, Chai H, Gupta MP, et al. Cytotoxic biflavonoids from Selaginella willdenowii. Phytochem. 1096; 40:129-134.
Miao N, Tao H, Tong C, et al. The Selaginella tamariscina (Beauv.) Spring complex in the treatment of experimental diabetes and its effect on blood rheology. Zhongguo Zhong Yao Za Zhi. 1996; 21:493-512.
Lee IS, Nishikawa A, Furukawa F, et al. Effects of Selaginella tamariscina on in vitro tumor cell growth, p53 expression, G1 arrest and in vivo gastric cell proliferation. Cancer Lett. 1999; 144:93-99.
Rojas A, Bah M, Rojas JI, et al. Spasmolytic activity of some plants used by the Otomi Indians of Queretaro for the treatment of gastrointestinal disorders. Phytomed. 1999; 6:367–371.
Ma SC, But PP, Ooi VE, et al. Antiviral amentoflavone from Selaginella sinensis. Biol Pharm Bull. 2001; 24:311-312.
Sah NK, Singh SN, Sahdev S, et al. Indian herb ‘Sanjeevani’ (Selaginella bryopteris) can promote growth and protect against heat shock and apoptotic activities of ultra violet and oxidative stress. J Biosci. 2005; 30: 499-505.
Cao Y, Tan NH, Chen JJ, et al. Bioactive flavones and biflavones from Selaginella moellendorffii Hieron. Fitoterapia 2010; 81:253-258.
Kang SS, Lee JY, Choi YK, et al. Neuroprotective effects of naturally occurring biflavonoids. Bioorg Med Chem Lett. 2005; 15:3588-3591.
Woo ER, Pokharel YR, Yang JW, et al. Inhibition of nuclear factor-kB activation by 20, 800-biapigenin. Biol Pharm Bull. 2006; 29:976-980.
Jing Y, Tang AZ, Liu J, et al. Effects of Selaginella doederleinii on human nasopharyngeal carcinoma TW03 cells in vitro and its mechanism. Zhong Yao Cai. 2009; 32:1864-1867.
Okigawa M, Hwa CW, Kawano N, Rahman W. Biflavones in Selaginella species. Phytochem. 1971; 10:3286-3287.
Arwa PS, Zeraik ML, Ximenes VF, Da Fonseca LM, Bolzani VS, Silva DHS. Redox-active biflavonoids from Garcinia brasiliensis as inhibitors of neutrophil oxidative burst and human erythrocyte membrane damage. J Ethnopharmacol. 2015; 174:410-418.
Abdallah HM, Almowallad FM, Esmat A, Shehata IA, Abdel-Sattar EA. Anti-inflammatory activity of flavonoids from Chrozophora tinctoria. Phytochem Lett. 2015; 13:74-80.
Park NH, Lee CW, Bae JH, Na YJ. Protective effects of amentoflavone on Lamin A-dependent UVB-induced nuclear aberration in normal human fibroblasts. Bioorg Med Chem Lett. 2011; 21:6482-6484.
Ndongo JT, Issa ME, Messi AN, Mbing JN, Cuendet M, Pegnyemb, DE Bochet, CG. Cytotoxic flavonoids and other constituents from the stem bark of Ochna schweinfurthiana. Nat. Prod. Res. 2015; 29:1684-1687.
Coulerie P, Nour M, Maciuk A, Eydoux C, Guillemot JC, Lebouvier N, et al., Structure-activity relationship study of biflavonoids on the Dengue virus polymerase DENV-NS5 RdRp. Planta Med. 2013; 79:1313-1318.
Hwang IS, Lee J, Jin HG, Woo ER, Lee DG. Amentoflavone stimulates mitochondrial dysfunction and induces apoptotic cell death in Candida albicans. Mycopathologia 2012; 173:207-218.
Zhang Z, Sun T, Niu JG, He ZQ, Liu Y, Wang F. Amentoflavone protects hippocampal neurons: Anti-inflammatory, antioxidative and antiapoptotic effects. Neural Regen. Res. 2015; 10:1125-1133.
Zheng XK, Liu CX, Zhai YY, Li LL, Wang XL. Feng WS. Protection effect of amentoflavone in Selaginella tamariscina against TNF-α-induced vascular injure of endothelial cells. Acta Pharm Sin. 2013; 48:1503-1509.
Vikram PK, Malvi R, Jain DK. Evaluation of analgesic and anti-inflammatory potential of mimosa pudica linn. Int J Curr Pharm Res 2012; 4 (4):47-50.
Nayak A, Garg M, Jain S, Khan MA, Jain DK, Khan N. Anti-Urolithiatic and Invitro-Invivo Anti-Oxidant Effects of Methanolic Extract of Thunbergia laurifolia on Ethylene Glycol-Induced Kidney Calculi in Rats. Sch Acad J Pharm. 2019; 8 (3):94-104.
Joshi S, Parkhe G, Aqueel N, Dixit N, Jain DK. Estimation of total phenolic, total flavonoids and total protein content of hydroalcoholic extract of Anacyclus pyrethrum. Pharmacologyonline 2019; 1:27-33.
Jain DK, Nayak A, Patel P, Jain S, Khan MA. Appraisal of in vitro Antioxidant and in vivo Anti-Inflammatory Activities of Various Extracts from the Fruits of Vitis vinifera L. Sch Acad J Pharm 2019; 8 (3):86-93.
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