Analytical method development and validation for the determination of Brinzolamide by RP-HPLC

  • Balakrishna Tiwari Research Scholar, Pacific University, Udaipur, India
  • Mrunal K. Shirsat Dean/Principal, PAHER University, Udaipur, India
  • Amol Kulkarni Director, Institute of Pharmaceutical Science and Research (for girls), Swami Chincholi, Daund, Maharashtra, India
DOI https://doi.org/10.22270/jddt.v10i1.3845

Abstract

Brinzolamide is inhibitor of carbonic anhydride and is highly specific and non-competitive. The aim of the present study is to develop a simple, precise, accurate, sensitive RP-HPLC method for the determination of bulk drug. The objective of the method validation is to demonstrate whether the method was suited for the intended purpose. The method was validated as per the ICH guidelines. The method was validated for linearity, precision (repeatability, intermediate precision), accuracy, specificity, robustness, ruggedness, limit of detection and limit of quantification. Cosmosil (4.6X250mm, 5 μ) column was used for separation. The selected wavelength for Brinzolamide was 254 nm. The mobile phase consists of Acetonitrile: Potassium dihydrogen phosphate buffer (40:60). Flow rate was delivered at 1.0 mL/min. Appropriate dilutions of standard stock solutions were prepared to get desired concentrations in the range of 100-500 mcg/ml. The equation od standard curve was y = 441.8x + 1132 and R2 = 0.998. The RT obtained was 6.6167 minutes.


Keywords: Brinzolamide, UV spectroscopy, RP-HPLC, ICH

Keywords: Brinzolamide, UV spectroscopy, RP-HPLC, ICH

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1.
Tiwari B, Shirsat MK, Kulkarni A. Analytical method development and validation for the determination of Brinzolamide by RP-HPLC. JDDT [Internet]. 15Jan.2020 [cited 18Apr.2024];10(1):92-6. Available from: https://jddtonline.info/index.php/jddt/article/view/3845
Issue
Vol 10 No 1 (2020): Volume 10, Issue 1, Jan-Feb 2020
Section
Research
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