Isolation, Purification and Quantification of Enterocin from Enterococcus Species and Potential antibacterial activity
Abstract
Enterococcus is a large genus of lactic acid bacteria of the phylum Firmicutes. Enterococci are Gram-positive cocci that often occur in pairs (Diplococci) or short chains. The purpose of this study was to determine the antimicrobial activity and occurrence of Enterocin in Enterococcus species isolated from different clinical samples. In this study, isolate Enterococcus in nutrient agar media. Perform biochemical testing and gram staining for identification of Enterococcus and sub culture in BHI (Brain Heart Infusion) and LAPTg broth. Enterocin was isolated from broth by different spectral techniques and used as an antibiotic against selective bacteria (Staphylococcus aureus, Bacillus, Escherichia coli and Streptomyces).
Keywords: - Clinical Samples; Enterococcus Spp.; Enterocin; Catalase; SDS-PAGE.
DOI
https://doi.org/10.22270/jddt.v9i4-A.3452Published
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