Physicochemical evaluation, in vitro anti-inflammatory, in vitro anti-arthritic activities and GC-MS analysis of the oil from the leaves of Gaultheria fragrantissima Wall of Meghalaya
Abstract
The aim of our present study is to assess the in vitro anti-inflammatory activity against denaturation of proteins whereby the test oil sample at different concentrations was incubated with egg albumin and the absorbance was determined at 660 nm. The anti-arthritic activity was investigated by two methods; firstly, in-vitro method by bovine serum protein denaturation and the absorbance was measured at 255 nm and secondly in vitro method by egg albumin denaturation where the absorbance was measured at 660 nm. In all the studies diclofenac sodium was used as thestandard drug. The physicochemical parameters like colour, solubility, refractive index, boiling point, specific gravity, carbon residue, iodine value, acid value, ester value were evaluated. The different components of the volatile oil were determined by GC-MS analysis. Ten organic compounds were identified out of which Methyl salicylate C8H8O6 was found to be the most dominant organic compound of Gaultheria fragrantissima oil (97.7%). The present results exhibited a concentration dependent inhibition of protein (albumin) denaturation by the test oil. The study reveals that diclofenac sodium was less effective when compared with the test oil. From the present findings it can be concluded that the essential oil of Gaultheria fragrantissima from Meghalaya possessed significant anti-inflammatory and anti- arthritic effects against the denaturation of protein in vitro. This effect could be due to the high content of methyl salicylate (97.7%) which is an inflammation-fighting compound. The high altitude and conducive climatic conditions of Meghalaya make wintergreen from this region far more superior in comparison to the ones grown in other parts of the world.
Keywords: Gaultheria fragrantissima, anti-inflammatory, anti-arthritic, GC-MS, methyl salicylate.
DOI
https://doi.org/10.22270/jddt.v9i3-s.2819References
Guenther E. The Essential Oils; van Nostrand Co., Inc.: New York, NY, USA (1950)
Bakkali F, Averbeck S, Averbeck D, Idaomar M. Biological effects of essential oils - a review. Food Chemistry and Toxicology 46: 446–75 (2008)
Bassolé I. H. N., & Juliani H. R.. Essential oils in combination and their antimicrobial properties. Molecules 17(4): 3989-4006 (2012).
Pranaybantawa and Mondal TK, Population studies of Gaultheria fragrantissimaWall in Darjeeling district of West Bengal. Natural Product Radiance. 2008; 7(1):68-73.
(Ayesha &Borthakur 2005.Ethnobotanicalwisdom of Khasi (Hynniewtreps) of Meghalaya,), Shiva offset press, Dhradun,pp 133.
Essien, E.P., J.P. Essien, B.N. Ita and G.A. Ebong,. Physicochemical Properties and Fungitoxicity of the Essential Oil of Citrus medica L. against Groundnut Storage Fungi. Turk. J. Bot., 2008; 32:161-164.
Nkafamiya, H. M. Maina, S. A. Osemeahon and U. U. Modibbo, Percentage oil yield and physiochemical properties of different groundnut species (Arachishypogaea), African Journal of Food Science, 2010; 4(7):418 - 421.
Bamgboye, A.I. and O.I. Adejumo, Physicochemical properties of Roselle seed oil. Nutrul and Food Science, 2010; 40(2):186-192.
Juven, B.J., kanner et al., Factors that interact with the antibacterial action of thyme essential oil and its active constituients. Journal of applied bacteriology 1994; (6):626-631.
Juliani, H.R., J.A. Zygadlo, R. Scrivanti, E. Sota and J.E. Simon, The essential oil of Anemiatomentosa (Savigny) Sw. var. anthriscifolia (Schrad). Mickel. FlavourFragr. J., 2004; 19: 541-543.
AOAC (Association of Official Analytical Chemists), 2000.Official methods of analysis. Gaithersburg, MD, Washington, USA.
Ambert, R.J.W , P.N.Skandamis, et al. A study of minimum inhibitory concentration and mode of action of oregano essential oil, thymol and carvacrol.Journal of applied microbiology 2001; 91(3): 453-462.
Charles D.J. and J.E. Simon Comparison of extraction methods for rapid determination of essential oil content and comparison of basil. ’Journal of the American Society for Horticulture Science 1990; 115(3); 458-462.
Sokovi , M and L.j.l.d. van Griensven, ‘Antimicrobial activity of essential oils and their components against the three major pathogens cultivated button mushroom, agaricusbiporus.’ European Journal of plant pathology 2006; 116(3)211-224.
Khajeh, M., Yamini Y, et al. Comparison of essential oils compositions of Ferula assa-foetida obtained by supercritical carbon dioxide extraction and hydro-distillation methods.’ Food chemistry 2005; 91(4):639-644.
Inouye S., Takizawa T, et al. Antibacterial activity of essential oils and their major constituients against respiratory tract pathogens by gaseous contact.’ Journal of Antimicrobial Chemotheraphy 2001; 47(5):565_573.
Khoddami A., Ghazali HM, A. Yassoralipour, Y. Ramakrishnan and A. Ganjloo, Physicochemical Characteristics of Nigella Seed (Nigella sativa L.) Oil as Affected by Different Extraction Methods. Journal of American Oil Chemical Society, 88: 533-540.
Chandra S, Chatterjee P, Dey P, Bhattacharya S. Phcog. J.,2012; 4,29:47-49.
Habibur Rahman, M. ChinnaEswaraiah and A.M. Dutt, In-vitroAnti-inflammatory and Anti-arthritic Activity of Oryzasativa Var. Joha Rice (An Aromatic Indigenous Rice of Assam), American-Eurasian J. Agric. & Environ. Sci., 2015; 15(1):115-121.
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