Characterization and phylogenetic analysis of alkaline α-amylase producing Brevibacillus laterosporus from mountain climatic zone of India
α-amylases (EC188.8.131.52) are glycoside hydrolases that breakdown complex starch and maltodextrins into glucose and maltose by acting upon 1,4-glycosidiclinkages. Several amylases have been isolated and purified from members of Bacillus community, which find extensive application in starch processing, textile and pharmaceutical industry. Keeping this in mind we isolated α-amylase producing gram positive bacterium from soils collected from mountain climatic zone of India and identified it as Brevibacillus laterosporus. We further studied the effect of temperature and pH on the amylase activity of this strain and found a very stable activity at alkaline pH of 10 and temperature of 45 ºC. To our knowledge this a first report on characterization and evolutionary analysis of alkaline α-amylase producing Brevibacillus laterosporus isolated from unexplored sites of mountain climatic zone of India.
Keywords: Climatic zone, Brevibacillus, Amylase, 16S rRNA gene sequencing, Phylogenetic analysis
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