Assessment of anti bacterial screening of Pongamia pinnata stem against bacterial species: An In-vitro approach
Numerous studies have shown that aromatic and medicinal plants are sources of diverse nutrient and non-nutrient molecules which protect the human body against various pathogens. Nature has been a source of medicinal agents for thousands of years and a large number of modern drugs have been isolated from natural sources. Herbal medicine is the oldest known healthcare system known to mankind. India has rich medicinal plants of nearly 7500 species. Many medicinal plants were with a long history of use in folk medicine against a variety of diseases. Recently, many researchers have taken a great interest on medicinal plants for their phytochemical constituents and biological activities including anti microbial activity. The anti bacterial activity of the ethanolic crude stem extract of Pongamia pinnata against four bacterial species (Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtilis and Escherichia coli) was investigated, using agar diffusion technique. At concentrations ranging from 10-40 mg/mL, the ethanolic crude extract showed activity against the four bacteria (Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtilis and E. coli) from 2 mm-20 mm, after 24 hours incubation. The present study showed the effectiveness of the crude plant extract against the tested bacterial strains and indicates the potential use of the extract as anti bacterial agent for the control of infectious diseases.
Keywords: Pongamia pinnata, anti bacterial activity, agar diffusion technique, bacterial strains, chloramphenicol.
2. Al-Bari MA, Sayeed MA, Rahman MS, Mossadik MA. Characterization and antimicrobial activities of a phenolic acid derivative produced by Streptomyces bangladeshiensis, a novel species collected in Bangladesh. Research Journal of Medicine and Medical Sciences 2006; 1:77-81.
3. Zy EA, Area A, Aam K. Antimicrobial activity of some medicinal plant extracts in Palestine. Pakistan Journal of Medical Sciences 2005; 21:187-93.
4. Rojas JJ, Ochoa VJ, Ocampo SA, Munoz JF. Screening for antimicrobial activity of ten medicinal plants used in Colombian folkloric medicine: A possible alternative in the treatment of non-nosocomial infections. BMC Complementary and Alternative Medicine 2006; 6:2.
5. Adriana B, Almodóvar1 ANM, Pereiral CT, Mariângela TA. Antimicrobial efficacy of Curcuma zedoaria extract as assessed by linear regression compared with commercial mouthrinses. Brazilian Journal of Microbiology 2007; 38: 440-5.
6. Vijaya K, Ananthan S. Microbiological screening of Indian medicinal plants with special reference to enteropathogens. Journal of Alternative and Complementary Medicine1997; 3:13-20.
7. Dilhuydy JM. Patients attraction to complementary and alternative medicine (CAM): a reality which physicians can neither ignore nor deny. Bull Cancer 2003; 90:623-8.
8. Krentz AJ, Bailey CJ. Oral antidiabetic agents: current role in type 2 diabetes mellitus. Drugs 2005; 65:385-411.
9. Gupta YK and Briyal S. Animal models of cerebral ischemia for evaluation of drugs. Indian J Physiol Pharmacol 2004; 48:379–94.
10. Amadou CK. Promoting alternative medicine. Africa Health J 1998; 2:20-5.
11. Krishnamurthi A. The Wealth of India, vol. VIII. Publication and Information Directorate CSIR, New Delhi, India, 1969.
12. Punitha R and Manoharan S. Antihyperglycemic and antilipidperoxidative effects of Pongamia pinnata (Linn.) Pierre flowers in alloxan induced diabetic rats. J Ethnopharmacol 2006: 105:39–46.
13. Naik M, Meher L, Naik SN and Dasa LM. Production of biodiesel from high free fatty acid Karanja (Pongamia pinnata) oil. Biomass and Bioenergy 2008; 32:354–7.
14. Sarma AK, Konwer D and Bordoloi PK. A comprehensive analysis of fuel properties of biodiesel from Koroch seed oil. Energy Fuels 2005; 19:656–7.
15. Meera B, Kumar S, Kalidhar SB. A review of the chemistry and biological activity of Pongamia pinnata. J Medicinal Aromatic Plant Sci 2003; 25:441–65.
16. Shoba GF, Thomas M. Study of antidiarrhoeal activity of four medicinal plants in castor-oil induced diarrhoea. J Ethnopharmacol 2001; 76(1):73–6.
17. Brijesh S, Daswani PG, Tetali P. Studies on Pongamia pinnata (L.) Pierre leaves: understanding the mechanism(s) of action in infectious diarrhea. J Zhejiang Univ Science B 2006; 7(8):665–74.
18. The Ayurvedic Pharmacopeia of India, Part-1, Vol-2, The Controller Publications, New Delhi, 1999:76-83.
19. Prajapati NS, Purohit SS, Shara AK, Kumar T. A Handbook of Medicinal plants, Agrobios India, Jodhapur. 2003:418.
20. Narender Boggula et al. Phytochemical investigation and in-vitro anti bacterial activity of dried leaves of Aerva lanata. Indo American Journal of Pharmaceutical Sciences 2016; 3(6):637-43.
21. Narender B, Swetha Reddy NR, Teja Sri A, Azmath F, Jainendra B, Vasudha B. Phytochemical evaluation and in vitro anti bacterial activity of dried seeds of Abrus precatorius. International journal of pharmaceutical sciences review and research 2017; 44(1):101-7.
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