Phytochemical and High-Performance Liquid Chromatography Analysis of Extract of Vernonia cinerea
Abstract
The present study aims to screen and quantify hydroalcoholic extract for phytochemical content and HPLC profiles for standardization. HPLC was carried out using a RP-C18 analytical column with a mobile phase composed of acetonitrile: methanol (50:50 v/v) and was isocratically eluted at a flow rate of 1 mL min-1. A small sample volume of 20 μL was used for each sample run, being injected into the HPLC system. The chromatogram was monitored with UV detection at a wavelength of 256 nm. Phytochemical screening of the extract showed the presence of flavonoids, amino acids, carbohydrates and protiens in hydroalcoholic extract. Quantification of total flavonoids showed that hydroalcoholic extract of Vernonia cinerea had flavonoid content 0.547 mg/100mg equivalent to quercetin. The data presented here could be used for the standardization of hydroalcoholic extract of Vernonia cinerea, either for future studies or in herbal drug formulations.
Keywords: Vernonia cinerea, HPLC profiling, Phytochemical analysis, Hydroalcoholic extract.
DOI
https://doi.org/10.22270/jddt.v9i1.2227References
Cowan M.M., Plant products as antimicrobial agents. Clin. Microbiol. Rev., 1999; 12(4): 564-582.
Butu M., Rodino S., Butu A., Butnariu M., Screening of bioflavonoid and antioxidant activity of Lens culinaris medikus. Digest Journal of Nanomaterials and Biostructures, 2014; 9(2): 519-529.
Ahmed D., Khan M.M., Saeed R., Comparative Analysis of Phenolics, Flavonoids, and Antioxidant and Antibacterial Potential of Methanolic, Hexanic and Aqueous Extracts from Adiantum caudatum Leaves. Antioxidants, 2015; 4:394-409.
Tshivhandekano I., Ntushelo K., Ngezimana W., Tshikalange T.E., Mudau F.N., Chemical compositions and antimicrobial activities of Athrixia phylicoides DC. (bush tea), Monsonia burkeana (special tea) and synergistic effects of both combined herbal teas. Asian Pac. J. Trop. Med., 2014; 7: S1:S4: 48-53.
Yusuf M, Chowdhury JU, Wahab MA, Begum J. Medicinal plants of Bangladesh. BCSIR, Dhaka (1994) 17–266.
Latha LY, Darah I, Jain K, Sasidharan S. Toxicity study of Vernonia cinerea. Pharmaceutical Biology. 2010; 48 (1):101-104.
Adeboye JO, Asije W, Awe SO. Diuretic and antidiuretic activity of the leaf extracts of Vernonia cinerea. Phytotherapy research. 1997; 11(6):454-456.
Iwalewa EO, Iwalewa OJ, Adeboye JO. Analgesic, antipyretic, anti-inflammatory effects of methanol, chloroform and ether extracts of Vernonia cinerea less leaf. Journal of Ethnopharmacology. 2003; 86:229- 234.
Ganesh P, Kumar KV, Kumar HS. Antidiarrhoeal activity of methanolic extract of V. Cinerea leaves less on female albino rats. International Research Journal of Pharmacy. 2011; 2 (5):211-213.
Rizvi SMD, Biswas D, Arif JM, Zeeshan M. In-vitro antibacterial and antioxidant potential of leaf and flower extracts of Vernonia cinerea and their phytochemical constituents. International Journal of Pharmaceutical Sciences Review and Research. 2011; 9 (2):164-169
Arivoli S, Tennyson S, Martin JJ. Larvicidal efficacy of Vernonia cinerea (L.) (Asteraceae) leaf extracts against the filarial vector Culex quinquefasciatus Say (Diptera: Culicidae). Journal of Biopesticides. 2011; 4 (1):37-42
Kumar PP, Kuttan G. Vernonia cinerea L. scavenges free radicals and regulates nitric oxide and proinflammatory cytokines profile in carrageenan induced paw edema model. Immunopharmacology and Immunotoxicology. 2009; 31(1):94-102.
Kokate CK. Practical Pharmacognosy. 4th edition. Delhi: Vallabh Prakashan; 1994.
Harborne JB. Phytochemical methods. London: Chapman and Hall; 1973.
Mervat MM, El Far, Hanan AA. Antioxidant activities, total anthocyanins, phenolics and flavonoids contents of some sweet potato genotypes under stress of different concentrations of sucrose and sorbitolAustralian J Basic Applied Sc. 2009; 3:3609-3616.
Published
Abstract Display: 837 
PDF Downloads: 917 How to Cite
Issue
Section
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0). that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
How to Cite
.