Physicochemical, Qualitative and Quantitative Determination of Secondary Metabolites and Antioxidant Potential of Kalanchoe Pinnata (Lam.) Pers. Leaf Extracts
Abstract
Kalanchoe pinnata (Lam.) Pers. has many pharmacological properties such as anticancer, antidiabetic, insecticidal, antimicrobial, anti-urolithiatic, etc. The plant leaves are used as ethno medicine traditionally. Many diseases are associated with oxidative stress caused by free radicals. The aim of the present study was to evaluate physicochemical, qualitative and quantitative phytochemical analysis and in vitro antioxidant activities of leaf of Kalanchoe pinnata collected from Bhopal region of Madhya Pradesh.The physicochemical evaluations carried out in terms of loss on drying, ash value, extractive values and acid insoluble ash value ect. Qualitative analysis of various phytochemical constituents and quantitative analysis of total phenolics and flavonoids were determined by the well-known test protocol available in the literature. Quantitative analysis of phenolic and flavonoids was carried out by FolinsCiocalteau reagent method and Aluminium chloride method respectively. The In vitro antioxidant activity of ethanolic extract of the leaf was assessed against DPPH assay method using standard protocols. Phytochemical analysis revealed the presence of phenols, flavonoids, tannins, saponins, alkaloids. The total phenolics content of leaves ethanolic extract was (4.63mg/100mg), followed by flavonoids (2.03mg/100mg). The activities of ethanolic leaves extract against DPPH assay method were concentration dependent with IC 50 values of ascorbic acid and extracts 24.44 and 49.81μg/ml respectively. These studies provided information for standardization and correct identification of this plant material. The diverse array of phytochemicals present in the plant thus suggests its therapeutic potentials which may be explored in drug manufacturing industry as well as in traditional medicine.
Keywords: Kalanchoe pinnata, Physicochemical, Qualitative, Quantitative phytochemical, Antioxidant
Downloads
References
2. WHO. Traditional medicine-growing needs and potentials. WHO Policy Perspectives Med, 2002.
3. WHO. Traditional Medicine and Modern Health Care Progress Report by the Direct General World Health Organization, Document No A-144110, 1991.
4. Sneader W, The discovery of aspirin: A reappraisal, BMJ (Clinical research ed., 2000; 321(7276):1591-1594.
5. Aguoru CU, Ameh SJ, Olasan O. Comparative phyochemical studies on the presence and quantification of various bioactive compounds in the three major organs of okoho plant (cissuspopuneaguill&perr) in Benue State, North Central Nigeria, West Africa, European Journal of Advanced Research in Biological and Life Sciences, 2014; 2(2):16-22.
6. Sindhu S, Manorama S. Exploration of Antioxidant Properties in Various Extracts of Bryophyllumpinnatum (Lank.). Research in Pharmacy 3(4): 01-08, 2013.
7. Nwali BU, Okaka ANC, Ibiam UA, Aja PM. Phytochemical Composition of BryphyllumPinnatum leaves. International Journal of Advanced Biological Research 2012; 2: 614-616.
8. Umebese CE, Falana FD. Growth, phytochemicals and antifungal activity of Bryophyllumpinnatum L. subjected to water deficit stress: African Journal of Biotechnology 2013; 12: 6599-6604.
9. Dassharma K, Bhatkar A, Pandey P, Shaikh N. Bryophyllumpinnatum (Lam): A potential source of antioxidants. Bionano Frontier 2013; 6:140-144.
10. Kamboj A, Saluja AK. Bryophyllumpinnatum (Lam.) Kurz.: Phytochemical and pharmacological profile. A review Phcog Rev 2009; 3: 364-374.
11. Mensor LL, Menezes FS, Leitão GG, Reis AS, dos Santos TC, et al. Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytother Res2001; 15: 127-130.
12. Ruqaiyah Khan, Afzal M, Kazmi I, Chauhan M, Bisht T. Chemical composition of bryophyllum. International Journal of Research in Biological Science 2012; 2:143-149.
13. Quality standards of Indian medicinal plants. Medicinal Plants Unit, ICMR, New Delhi, 2013, 11.
14. WHO Quality control methods for medicinal plants. Geneva, 2002, 28-31.
15. Government of India-Ministry of Health and Family Welfare. Indian Pharmacopoeia, Edn. 1, India Controller of Publications, New Delhi, 1996, II
16. Harborne JB. Phytochemical Methods: A Guide to Modern techniques of plant analysis, Edn 2, Chapman and Hall, London, 1984, 123-75.
17. Mukherjee PK. Quality control of herbal drugs. 2nd Ed. Business Horizons; 2007.
18. Khandelwal KR. Practical pharmacognosy technique and experiments. 23rd Ed. NiraliPrakashan; 2005.
19. Kokate CK. Practical pharmacognosy. 4th Ed. Vallabh Prakashan; 1994.
20. Olufunmiso OO, Afolayan AJ. Phenolic content and antioxidant property of the bark extract of Ziziphusmucronatawilld. Subsp. mucronatawilld, BMC Complement Alternative Medicine, 2011; 11:130.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0). that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
.