FORMULATION, CHARACTERIZATION AND EVALUATION OF ANTI-INFLAMMATORY AND ANTI-ANGIOGENIC ACTIVITIES OF MEMECYLAENE NANOEMULSION
Abstract
The present study was undertaken to formulate and evaluate the anti-inflammatory, anti-oxidant and anti-angiogenic activities of nanoemulsion of Memecylaene. Memecylaene was isolated from the leaves of Memecylon malabaricum by using various chromatographic methods. An oil-in-water (O/W) nanoemulsion of Memecylaene was formulated by sonication method using sunflower oil (oil phase), Tween 80 (Surfactant) and Ethanol (co-surfactant). The prepared nanoemulsion was characterized for its droplet size, poly dispersity index and zeta potential. Stability studies were performed and the nanoemulsions were subjected to different biological activities. The formulated nanoemulsion had a particle size range of 52.02 nm to 59.47 nm and zeta potential of -1.27 mV. The enhanced activity of Memecylaene, encapsulated in O/W emulsions is evidenced by the inhibition of phospholipase (PLA2) enzyme and H+, K+ -ATPase and thus showing anti-inflammatory and anti-secretagogues effects. The in vitro anti-oxidant activity was evaluated by DPPH radical and Nitric oxide radical scavenging activity. Further, the inhibition of the growth of neo vessels formation in the in-vivo model system of chick chorioallantoic membrane (CAM) assay, which is angiogenesis dependent, was also observed. The above findings would help in understanding the putative potential of Memecylaene-loaded nanoemulsion as a therapeutic agent.
Keywords: Anti-angiogenesis, Anti-oxidant, Gastric (H+ K+), Memecylaene, Nanoemulsion, Phospholipase A2 (PLA2).
DOI
https://doi.org/10.22270/jddt.v8i5-s.1915References
Bharathi TR, Sampath Kumar KK, Prakash HS. memecylon species: a review of traditional information and taxonomic description. International Journal of Pharmacy and Pharmaceutical Sciences, 2016; 8(6):27-34.
Rekha ND, Gowda TV, Aradhya SM, Suresha RN, Jayashree K. Anti-inflammatory properties of Memecylaene: A novel compound isolated from Memecylaene Malabaricum. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 2014; 5:1645-1654.
Shafiq S, Shakeel F, Talegaonkar S, Ahmad FJ, Khar RK, Ali M. Development and bioavailability assessment of Ramipril nanoemulsion formulation. European Journal of Pharmaceutics and Biopharmaceutics, 2007; 66:227-243.
Nagarajan R, Ruckenestein E. Molecular theory of microemulsions. Langmuir, 2000; 16:6400-6415.
Joyce NM, Nagarajan R. Recent research trends in fabrication and applications of plant essential oil based nanoemulsions. Journal of Nanomedicine and Nanotechnology, 2017; 8:1-10.
Srilatha R, Aparna C, Srinivas P, Sadanandam M. Formulation, evaluation and characterization of Glipizide nanoemulsion. Asian Journal of Pharmaceutical and Clinical Research, 2013; 6:66-71.
Rajasree PH, Willi P, Chandra P S, Riaz A O, Umme H, Atul S. Eudragit encapsulated cationic poly(lactic-co-glycolicacid) nanoparticles in targeted delivery of capecitabine for augmented colon carcinoma therapy. Journal of Drug Delivery Science and Technology. 2018; 46:302-311.
Boman HG, Kaletta U. Chromatography of rattlesnake venom: A separation of three phosphodiesterases. Biochimica et Biophysica Acta, 1957; 24:619-631.
Mohamed R, Dharmappa KK, Tarannum S, Jameel NM, Kannum SA, Ashrafulla HS et al. Chemical modification of ascorbic acid and evaluation of its lipophilic derivatives as inhibitors of secretory phospholipase A2 with anti-inflammatory activity. Molecular and Cellular Biochemistry, 2010; 345:69-76.
Im WB, Sih JC, Blakeman DP, Megrath JP. Omeprazole, a specific inhibitor of Gastric (H+, K+) ATP ase is a H+ activated oxidizing agent of sulfhydryl groups, The Journal of Biological Chemistry.1985;260:4591-4597.
Tsai CM, Chen KY, Canellakis ES. Isolation and characterization of the plasma membrane of l-1210 cells. Iodination as a marker for the plasma membrane. Biochimica et Biophvsica Acta(BBA)-Biomembranes, 1975; 401:196-212.
Mallesha L, Kikkeri PH, Kikkeri NM, Rekha ND. In vitro anti-oxidant activity of 1-[5-(4-Methoxy-phenyl)- [1,3,4] oxadiazol-2-yl] piperazine derivatives. Canadian Chemical Transactions, 2014; 2:518-525.
Marcocci L, Packer L, Droy LMT, Sekaki A, Gardes AM. The anti-oxidant action of Ginko biloba extracts EGb761. Methods in Enzymology, 1994; 234:462-475.
Bushra BA, Rekha ND, Vasantha Kumar BC, Lakshmi RV, Khanum SA. Synthesis, characterization, biological and catalytic applications of transition metal complexes derived from Schiff base. Bioorganic and Medicinal Chemistry Letters, 2014; 24:3559-3564.
Yamaguchi T, Takamura H, Matoba T, Terao J. HPLC method for evaluation of the free radical scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl. Bioscience. Biotechnology and Biochemistry, 1998; 62:1201-1204.
Hullatti KK, Ravi VR. Anti-microbial activity of Memecylon Malabaricum leaves. Fitoterapia, 2004; 75:409-411.
Ramaiah M, Srikantha RK, Rao BG. Anti-diabetic activity of methanolic extract of Memecylon malabaricum (Melastomataceae) leaves. International Journal of Phytopharmcy, 2012; 10:160-166.
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