Indian toad (Bufo melanostictus, Schneider) skin extract induces apoptosis and shows cytotoxic effect on Ehrlich ascites carcinoma (EAC) cells
Abstract
Indian toad (Bufo melanostictus) skin extract (TSE) was evaluated for cytotoxic and apoptogenic activities on Ehrlich ascites carcinoma (EAC) cells of EAC bearing Swiss albino male mice. LD50 of TSE was found to be 400 mg kg-1, (i.v.) and 750 mg kg-1, (i.p.) in mice. EAC cells (1-2 ´ 105 / mouse) were inoculated (i.p.) into mice (20.6 ± 0.14 gm) and the status of EAC cell proliferation and viability were studied in control and TSE treated groups. TSE (10, 20, 30 mg kg-1 d-1, i.p. for 10 d) significantly inhibit EAC cell growth in dose dependent manner. TSE (50, 100 mg kg-1 d-1, i.p. for 1 d) significantly reduced the viability of EAC cells and decreased the MTT values compared to control cells. TSE induced DNA breakage was reflected in DNA ladder and single cell gel electrophoresis (comet assay). TSE significantly (P<0.001) increased the number tailed cells and length-width ratio of DNA mass in EAC cells as compared to control. Fluorescent microscopy of TSE treated cells showed significant increase in number of early and late apoptotic cells compared to the control EAC cells. Apoptotic index of TSE treated cells was significantly (P<0.001) higher than that of the control cells. Changes in serum LDH, β2-microglobulin in TSE treated EAC mice and our earlier studies indicated the involvement of immunomodulation which may indirectly be associated with the anticancer activity of TSE.
Keywords: Toad skin extract; Bufo melanostictus; Ehrlich ascites carcinoma; Cytotoxic agent;
DOI
https://doi.org/10.22270/jddt.v8i5.1873References
Hiteksha SP, Mamata BS; Thespesia populnea Linn., A review, Int J Pharmacognosy 2017; 4(1):1-05
Bhat Gopalakrishna K., Flora Of Udupi, Published by Indian Naturalist, Udupi, Edition 2003, 66
Nadakarni KM, Indian Materia Medica; Popular prakashana; PVT. Ltd., 1976, pp630
Khare CP, Indian Medicinal Plants, An illustrated dictionary; New Delhi; Springer; 2007; 659
Kirtikar KR & Basu BD. Indian Mediicnal Plants, Volume I, Dehradun; International Book Distributors; 2008, 341
Sharma PV, Classical uses of Medicinal plants, ChaukambhaBharati Academy, Varanasi, 2004, 239.
Mallya Suma V, Suchitra P, Vishwanatha U, Sunilkumar Narayana; Anatomical atlas of Panchavalkala- effective healing of Five bark drugs in gynecological disorders; J. Ayu. Herb Med, 2018; 4(1):6.
Mukharjee Pulok K, Quality Control of Herbal Drugs; New Delhi: Business Horizons; 2002; 68
Quality control methods for medicinal plants. Geneva: World Health Organization; 1998; 25-8
Devaiah KM, Venkatasubramanian P., Genetic characterization and authentication of Embeliaribes using RAPD-PCR and SCAR marker; Planta Med, 2008; 74:306-8
Bhagyawant SS, RAPD-SCAR Markeres; An interface tool for Authentication of traits; Journal of Biosciences and Medicines; 2016; 4:1-9.
Charu D et al., Development of male-specific SCAR marker in datepalm(Phoenix dactylifera L.); Tree Genetics and Genmics. 2013; 9:143-50
Marym Sarvat et al., A standardized protocol for genomic DNA isolation from Terminaliaarjuna for genetic diversity analysis; Electronic Journal of Biotechnology 2006; 9(1):87-91
Pirttila AM et al., DNA isolation methods for medicinal and aromatic plants; Plant Molecular Biology Reporter; 19(3) 2001pp 273 a-f.
Polashock James J, Development of SCAR markers for DNA fingerprinting and Germplasm analysis of American Cranberry; J. Amer. Soc. Hort. Sci. 2002; 127(4):677-84
Joshi D, Joshi G; Quality control and standardization of Ayurvedic medicines; Varanasi, Chaukamba orientalia 2011,p113
Kalpana Joshi et al., Molecular markers in herbal drug technology; Current Science 2004; 87(2):159.
Sunilkumar KN et al., Molecular fingerprinting of Helianthus elastica(Desr.) Danser growing on five different hosts by RAPD; Saudi Journal of Biological Sciences. 2016; 23:335-40.
Santoshkumar P et al. Application of DNA fingerprinting tools for authentication of ayuredic herbal medicine- A review; Journal of scientific and Innovative Research 2014; 3(6):606-12.
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