NEW ANALYTICAL METHODS FOR ESTIMATION OF ARTEETHER BY UV AND FLUORESCENCE SPECTROPHOTOMETRY: DEVELOPMENT AND VALIDATION
Abstract
The present research work discusses the development and validation of two different spectrophotometric methods for estimation of α-β arteether using UV spectrophotometer and spectrofluorimeter for the first time. Two simple, accurate, precise, sensitiveand economical methods has been developed and validated for the estimation of α-β arteether in bulk and pharmaceutical dosage form as per ICH guidelines Q2(R1). The solvent used for UV spectroscopy was methanol and HCl (8:2) and methanol was used for fluorimeter. For qualitative and quantitative analysis, 254 nm was used in UV spectroscopy and excitation and emission wavelengths were set at 354 nm and 697 nm respectively for fluorimetry. Coefficients of correlation were found to be 0.993 and 0.992 for UV spectroscopy and fluorimetry. Both methods show good accuracy and precision and were compared statistically by using two way ANOVA which shows no significant difference between these methods. So the proposed methods were found to have equal applicability for estimation and routine analysis of arteether in pharmaceutical formulations.
Keywords: Arteether, Analytical method, Fluorimeter, Pharmaceutical formulation, UV spectrophotometer.
DOI
https://doi.org/10.22270/jddt.v8i5.1797References
Asthana OP, Srivastava JS, Pandey TK, Vishwanathan KA, Dev V, Mahapatra KM, Nayak NC, Balsara AB, Mandal OP, Gupta N, Mishra SK, Multicentric clinical trials for safety and efficacy evaluation of alpha; beta arteether in complicated P. falciparum malaria, The Journal of the Association of Physicians of India. 2001; 49:155-1160.
Thakur S, Sharma S, Kumar S, Singh U, Baldi A, Artemisinin: The ‘Molecule of Hope’ for treatment of malaria, Pharm Aspire, 2013; 4:84-89.
Mandal PK, Sarkar N, Pal A, Efficacy of arteether in chloroquine resistant falciparum malaria in eastern India, Indian J. Med. Res, 2004; 119(1):28-32.
Agarwal SP, Ali A, Ahuja S, Estimation of artemether and arteether by HPTLC, Asian J.Chem, 2007; 19:4407- 4414.
Al-Angary AA, Bayomi MA, Khidr SH, Al-Meshal MA, Lutfi KM. Determination of arteether in plasma using a simple and rapid high-performance liquid chromatographic assay. Analytical letters. 1994; 27(14):2689-2702.
Rajanikanth M, Madhusudanan KP, Gupta RC, A HPLC-MS method for simultaneous estimation of α-β -arteether and its metabolite dihydroartemisinin, in rat plasma for application to pharmacokinetic study, Biomed.Chromatogr.,2004; 17(7):440-446.
ICH, Q2 (R1) Validation of analytical procedures: Text and methodology, International Conference on Harmonisation, 1996.
Jain D, Bhadra D, Bhadra S, Jain NK, Direct UV Spectrophotometric estimation of -arteether, Indian Journal of Pharmaceutical Sciences, 2005; 67(4):506-507.
Baldi A, Panwar M S, Swapnil G, Development and validation of spectrophotometric method for determination of ropinirole hydrochloride in bulk and formulation, Inventi Rapid: Pharm Analysis & Quality Assurance, 2015; 1:1-4.
Singh U, Baldi A, Simultaneous estimation of quercetin and silymarin: Method development and validation, International Journal of Pharmaceutical Biological Archive, 2013; 4(3):527-531.
Baldi A, Sanadya J, Patidar A, Method development and validation for estimation of quetiapinefumerate by RP-HPLC, Asian Journal of Research in Chemistry, 2010; 3(3):604-607.
Zulfiqar Ali, Neeraj Mishra, Ashish Baldi, Development and characterization of arteether loaded nanostructured lipid carriers for treatment of malaria, Artificial Cells, Nanomedicine and Biotechnology,2016; 44 (2):545-559
Published
Abstract Display: 881 
PDF Downloads: 946 How to Cite
Issue
Section
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0). that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
How to Cite
.