ANTI-ANAEMIC ACTIVITY OF HYDRO-ALCOHOLIC LEAF EXTRACT OF LUFFA AEGYPTIACA IN PHENYLHYDRAZINE INDUCED ANEMIC RATS

Abstract

The main aim of the current study is to determine the anti-anaemic activity in hydro-alcoholic leaf extract of Luffa aegyptiaca in phenylhydrazine induced anemic rats. Phenylhydrazine (60mg/kg) was administered intraperitoneally in rats for 2 days to induce anemia. The animals were divided into 5 groups containing 6 animals each. 1^st group was served as normal control group, 2^nd group was served as anaemic control administered with phenylhydrazine for two days, 3^rd group was served as standard reference control administered with Vitamin B₁₂ complex, 4^th group was served as test control administered with 100 mg/kg of hydro-alcoholic leaf extract of Luffa aegyptiaca and 5^th group was served as test control administered with 200mg/kg of hydro-alcoholic leaf extract of Luffa aegyptica. All the test drugs were given for 28 days daily through oral route. On 29^th day blood was withdrawn, through tail puncture and subjected to the determination of RBC, Hb and percentage Haematocrit. Both the hydro-alcoholic leaf extract of Luffa aegyptiacaand Vit. B₁₂ significantly increase the HB, RBC & % HCT level which indicates that Luffa aegyptiaca leaf exhibits’ the anti-anaemic activity.