IN VITRO AND IN VIVO EVALUATION OF MUCOADHESIVE MICROSPHERES FOR TREATMENT OF HELICOBACTER PYLORI USING FACTORIAL DESIGN
Abstract
The research involves characterization of in vitro and in vivo activity of mucoadhesive microspheres for Helicobacter pylori eradication. Amoxicillin and Famotidine were used as model drugs so that the dual therapy gives better H. pylori eradication. Preparation was carried by an emulsion-solvent evaporation method and 27 batches were prepared individually using 33 factorial designs to study the effect of independent variables on dependent variables. The in vitro mucoadhesion test and in vivo studies (Bacterial clearance study, in vivo mucoadhesion and in vivo ulcer index studies) were performed. A27 batch showed 66% mucoadhesion after 10 h and F24 showed 74% of mucoadhesion after 10 h. In the bacterial clearance studies, the mean bacterial count (log colony forming units) after oral administration of drug-loaded microspheres was found to be 3.72 ± 0.58. The drug-loaded microspheres formulation exhibited better clearance from infection than plain drugs solution at the same doses. Drug microspheres formulation was found to be effective in the treatment of H. pylori infections effectively, and in in vivo mucoadhesion studies, the developed system was well taken up and processed by the cells of gastric mucosa of the stomach.
DOI
https://doi.org/10.22270/jddt.v7i7.1589References
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