HYPOGLYCAEMIC ACTIVITY OF CENTRATHERUM ANTHELMINTICU IN EXPERIMENTAL ANIMALS
Abstract
The Effect of Ethanolic extracts of Centratherum anthelminticum on Diabetic rats Assessment of the antihyperglycemic effect of ethanolic extract of Centratherum anthelminticum seeds in normal and alloxan induced diabetic rats: The animals will be divided into nine groups and each group consisted of six rats. Group 1 as normal control. Group 2 as the diabetic control. Groups 3, 4 and 5 are normally treated with 0.25 g, 0.50 g and 0.75 g/kg BW of CAEt, respectively. Groups 6, 7 and 8 are diabetic rats treated with 0.25 g, 0.50 g, and 0.75 g/kg BW of CAEt, respectively. Group 9 are diabetic rats which received glibenclamide (0.02 g/kg BW) an oral hypoglycemic agent dissolved in distilled water. Normal control and the diabetic control rats are fed distilled water alone. After an overnight fast, the plant extract suspended in distilled water will feed to the rats by gastric intubation using a force feeding needle. Blood samples are collected for the measurement of blood glucose from the tail vein at 0, 1, 2, 3, 4, 5 and 6 h after the administration of plant extract and blood glucose levels are determined by Glucose oxidase – peroxidase method. The phytochemical analysis will be carried out in the ethanolic extract by different methods of phytochemical analysis.
Keywords: Hypoglycaemia, Diabetes mellitus, Centratherum anthelminticumThe effect of Ethanolic extracts of Centratherum anthelminticum on Diabetic rats Assessment of antihyperglycemic effect of ethanolic extract of Centratherum anthelminticum seeds in normal and alloxan induced diabetic rats: The animals will be divided in to nine groups and each group consisted of six rats. Group 1 as normal control. Group 2 as the diabetic control. Groups 3, 4 and 5 are normal treated with 0.25 g, 0.50 g and 0.75 g/kg bw of CAEt, respectively. Groups 6, 7 and 8 are diabetic rats treated with 0.25 g, 0.50 g and 0.75 g/kg bw of CAEt, respectively. Group 9 are diabetic rats which received glibenclamide (0.02 g/kg bw) an oral hypoglycemic agent dissolved in distilled water. Normal control and the diabetic control rats are fed distilled water alone. After an overnight fast, the plant extract suspended in distilled water will fed to the rats by gastric intubation using a force feeding needle. Blood samples are collected for the measurement of blood glucose from the tail vein at 0, 1, 2, 3, 4, 5 and 6 h after the administration of plant extract and blood glucose levels are determined by Glucose oxidase – peroxidase method.Phytochemical analysis will carried out in the ethanolic extract by different methods of phytochemical analysis.
Keywords: Hypoglycaemia, Diabetes mellitus, Centratherum anthelminticumDOI
https://doi.org/10.22270/jddt.v7i4.1472References
Carvalho E. N, Carvalho N. A. S, Ferreira L. M, “Experimental model of induction of diabetes mellitus in ratsâ€, Acta Cir Bras, 2003; (18):268-72
Handa S.S, Chawla A.C, Maninder A. “Hypoglycaemic Plants reviewâ€. Fitoterapia 1989; (60):195-222.
Holman R.R, Turner R.C, “oral agents and insulin in the treatment of NIDDMâ€, Textbook of diabetes Oxford Black, 1991; 469-470.
Ohkubo Y. Kishikawa, H. Araki, E. “intensive insulin therapy prevented the progression of diabetic microvascular complications in Japanese patients with non insulin dependent diabetes mellitus: a randomized prospective six year studyâ€, Diabetes res clin Prac. Pub Med 1995; (28):103.117.
Qureshi S.A, Nawaz, A. Udani S.K, Azmi, M.B, “Hypoglycaemic and hypolipidemic activities of Rauwolfia serpentina in alloxan-induced diabetic ratsâ€, Int J Pharmacol, 2009; (5):323-326.
World Health Organization, “second report of the WHO Expert committee on diabetes mellitusâ€, Geneva, Technical Report Series; 1980; 646-666.
Zhang, X.F, Tan, B.K.H, “Antihyperglycemic and antioxidant properties of Andrographis paniculata in normal and diabetic rats, clin Exp Pharmacol Physiol; (2002); 27(29):358-363.
Published
Abstract Display: 480 
PDF Downloads: 659 How to Cite
Issue
Section
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0). that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
How to Cite
.