ANTIOXIDANT ACTIVITY OF POMEGRANATE PEEL POWDER
Abstract
The aim of present study was to evaluate in-vitro anti-oxidant properties of Punica granatum fruit (Pomegranate fruit) peel. Antioxidants are molecules involved in defense mechanisms against the deleterious effects of free radicals in most organisms. Antioxidants are the agents responsible for scavenging free radicals. A number of methods are currently being used for the evaluation of the antioxidant and free-radical scavenging properties of natural and synthetic antioxidants, including the DPPH method. The Punica granatum fruit (Pomegranate fruit) peel powder suspension was prepared and the DPPH radical scavenging assay was the method adopted to determine antioxidant potentials of aqueous suspension of pomegranate peel powder. Results revealed that DPPH aqueous solution gave comparable free-radical activity 24 hours post preparation compared with the freshly prepared solution. After 24 hours, activity was greatly reduced. It is, therefore, recommended that freshly prepared DPPH solution should be used at all times; however for prolonged experimental schedules, the DPPH solution should be used within 24 hours post preparation, so as to give comparable results with the freshly prepared solution and avoid ambiguity in results interpretation. Aqueous suspension of peel powder showed good antioxidant effect. Phenolic compounds, tannins and flavonoids are the major phytochemicals present in the pomegranate peel. Percentage of inhibition increased with the increased concentration of extracts. The present study provides evidence that the Punica granatum fruit peels is potential source of natural antioxidant
Keywords: Antioxidant, Pomegranate peel powder suspension, DPPH, Free radical scavenging.
Keywords:
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https://doi.org/10.22270/jddt.v7i2.1380References
El-Desouky TA, Sherif MR, Sherif MS, Khayria NM, Protective effect of aqueous extract pomegranate peel against sterigmatocystin toxicity in rat, Journal of Drug Delivery & Therapeutics. 2015; 5(5):9-18.
Rajan S, Mahalakshmi S, Deepa V.M., Sathya K, Sajitha S, Thairunalasundari T. Antioxidant potentials of Punica granatum fruite rind extract. Int J Pharm Pharm Sci., 2011; 3:82-88.
Ren W, Qian Z,Wang H, Zhu L, Zhang L. Flavonoids: Promising anticancer agents. Medicinal Res Rev., 2003; 23(4):519-534.
Gil M.I., Tomas-Barberan F.A., Hess-Pierce B., Holcroft D.M., Kader AA. Antioxidant activity of pomegranate juice and its relationship with phenolic composition and processing. J Agric Food Chem., 2000; 48:4581-4589.
Negi P.S., Jayaprakasha G.K., Jena B.S. Antioxidant and antimutagenic activities of Pomegranate peel extracts. Food Chemistry. 2003; 80:393-397.
Singh R.P., Murthy K.N.C., Jayaprakasha G.K. Studies on antioxidant activity of pomegranate (Punica granatum) peel and seed extracts using in vitro models. J Agric Food Chem., 2002; 50: 81–86.
Aviram M., Rosenblat M., Bisgaier C.L., Newton R.S., Primo-Parmo S.L., La Du B.N. Paraoxonase inhibits high-density lipoprotein oxidation and preserves its functions. A possible peroxidative role for paraoxonase. J Clin Invest., 1998; 101:1581-90.
Aviram M., Rosenblat M., Gaitini D. Pomegranate juice consumption for 3 years by patients with carotidartery stenosis reduces common carotid intima-media thickness, blood pressure and LDL oxidation. Clin Nutr., 2004; 23: 423-433.
Sushil Kumar Middha, Talambedu Usha, and Veena Pande. A Review on Antihyperglycemic and Antihepatoprotective Activity of Eco-Friendly Punica granatum Peel Waste. Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicin., 2013, Article ID., 656172:1-10.
Eliana Harue Endo, Tânia Ueda-Nakamura, Celso Vataru Nakamura, Benedito Prado Dias Filho. Activity of Spray-dried Microparticles Containing Pomegranate Peel Extract against Candida albicans. Molecules. 2012; 17:10094-10107.
Himaja M., Anand Ranjitha, Ramana M.V., Anand M., Karigar Asif. Phytochemical Screening and Antioxidant Activity of Rhizome Part of Curcuma Zedoaria. Himaja Met al/ IJRAP. 2010; 1(2):414-417.
Kaur AD, Nain P, Nain J. In-vitro Antimicrobial and Antioxidant Activity of Ginkdo Biloba Bark Extract. IRJP. 2012; 3(6):116-119.
David A. Otohinoyi, Okobi Ekpo and Omodele Ibraheem. Effect of ambient temperature storage on 2,2-dipheny l-1-picrylhydrazyl (DPPH) as a free radical for the evaluation of antioxidant activity. Int. J. Biol. Chem. Sci., 2014; 8(3):1262-1268.
Brand-Williams W, Cuvelier M.E., Berset C.. Use of a free radical method to evaluate antioxidant activity. Lebenson Wiss Technol., 1995; 28(1): 25-30.
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