ANTIDIABETIC AND HYPOLIPIDEMIC EFFECT OF METHANOL EXTRACT OF STEREOSPERMUM COLAIS FRUIT IN STREPTOZOTOCIN INDUCED DIABETIC RATS
Abstract
Objective: To assess the antidiabetic and hypolipidemic properties of Stereospermum colais Fruit.
Methods: Acute toxicity test was done to check the toxicity of Stereospermum colais Fruit methanol extract at three dose levels was administered orally to streptozotocin (STZ) (40 mg/kg bw) induced diabetic rats for 15 days. The various parameters were studied including body weight, fasting blood glucose levels, plasma insulin, lipid profile, glycogen content, glycoslylated hemoglobin (HbA1c) and serum marker enzymes levels in normal, treated and diabetic rats. Histochemical analysis of pancreas was also carried out in normal treated and diabetic rats.
Results: The treatment group with the extract at three dose levels showed a significant increase in the liver, muscle glycogen and serum insulin level and a significant decrease in fasting blood glucose, glycosylated hemoglobin levels and serum marker enzyme levels. The total cholesterol and serum triglycerides levels were also significantly reduced and the high density lipoprotein level was significantly increased upon treatment with the Stereospermum colais Fruit methanol extract. Histochemical study of pancreas also confirmed the biochemical findings. Acute toxicity studies revealed the non-toxic nature of the Stereospermum colais Fruit methanol extract. Â Conclusions: The results of the experiments presented here suggest that methanol extract of Stereospermum colais Fruit exerts significant antidiabetic and hypolipidaemic effect in STZ induced diabetic rats.
Keywords: Stereospermum colais, Antidiabetic, Hypolipidemic, Fruit, streptozotocin.
DOI
https://doi.org/10.22270/jddt.v6i4.1272References
Qaseem A et al, Oral pharmacologic treatment of type 2 diabetes mellitus: a clinical practice guideline from the American College of Physicians. Annals of Internal Medicine, 2012; 156(3): 218-231.
Fonseca VA. Clinical diabetes: Translating research into practice. Philadelphia: Saunders Elsevier: 2006; 2-3.
kanchana G, Malini P, Rajadurai M, Antibiabetic Efficacy of Ellagic Acid in Streptozotocin induced Diabetes Mellitus in albino wistar rats, Asian Journal of Pharmaceutical and Clinical Research, 2011; 4(3): 124-128.
Pari L and Latha M, Effect of Cassia auriculata flowers on Blood Sugar Levels, Serum Tissue Lipids in Streptozotocin Diabetic rats, Singapore Medical Journal, 2002; 43: 617â€621.
Chaterjee A, The treatise on Indian medicinal plants. Published by National institute of science communication and information resources, 1997; 5: 46-47.
Vaidya SA, Indian medicinal plants a compendium of 500 species, published by orient Longman private limited, 1996; 5: 192.
Khadabadi SS, Deore SL, Baviskar BA, Experimental Phytopharmacognosy, 1st edition, Published by Nirali prakashan. 2011; 3.2- 3.8, 4.29-4.33.
Ghosh MN. Fundamentals of experimental pharmacology. Calcutta: Scientific Book Agency. 1984; 153.
Turner MA. Screening methods in pharmacology. NewYork: Academic Press. 1965; 26.
Bonner-Weir S. Morphological evidence for pancreatic polarity of beta-cells within islets of langerhans. Diabetes. 1988; 37(5): 616-621.
Triender P. Determination of glucose using glucose oxidase with an alternative oxygen acceptor. Ann Clin Biochem. 1969; 6: 24-27.
Carrol NV, Longley RW, Roe JH. The determination of glycogen in liver and muscle by use of anthrone reagent. J Biol Chem. 1956; 220(2): 583-593.
Sudhakar NS, Pattabiraman TN. A new colorimetric method for the estimation of glycosylated haemoglobin. Clin Chim Acta. 1981; 109(3): 267-274.
Reitman S, Frankel S. Colorimetric method for the determination of serum glutamic oxaloacetic acid and glutamic pyruvic transaminases. Am J Clin Pathol 1957; 28(1): 56-63.
Nirmala A, Saroja S, Vasanthi HR, Lalitha G. Hypoglycemic effect of Basella rubra in streptozotocin- induced diabetic albino rats. J Pharmacog Phytother 2009; 2: 25-30.
Mahimainathan L, Das F, Venkatesan B, Choudhury GG. Mesangial cell hypertrophy by high glucose is mediated by down regulation of the tumor suppressor PTEN. Diabetes 2006; 55: 2115-2125.
Rao NK, Nammi S. Antidiabetic and renoprotective effects of the chloroform extract of Terminalia chebula Retz. seeds in streptozotocin-induced diabetic rats. BMC Comp Alt Med 2006; 6: 17.
Shirwaikar A, Rajendran K, Barik R. Effect of aqueous bark extract of Garuga pinnata Roxb. in streptozotocin-nicotinamide induced type-II diabetes mellitus. J Ethnopharmacol 2006; 107: 285-290.
Dimo T, Rakotonirina SV, Tan PV, Azay J, Dongo E, KamtchouingP, et al. Effect of Sclerocarya birrea (Anacardiaceae) stem bark methylene chloride/methanol extract on streptozotocin-diabetic rats. J Ethnopharmacol 2007; 110(3): 434-438.
Punitha R, Manohara S. Antihyperglycemic and antilipidperoxidative effects of Pongamia pinnata (Linn.) Pierre flowers in alloxan induced diabetic rats. J Ethnopharmacol 2006; 105: 39-46.
Singh SK, Kesari AN, Gupta RK, Jaiswal D, Watal G. Assessment of antidiabetic potential of Cynodon dactylon extract in streptozotocin diabetic rats. J Ethnopharmacol 2007; 114:174-179.
Daisy P, Balasubramanian K, Rajalakshmi M, Eliza J, Selvaraj J. Insulin mimetic impact of Catechin isolated from Cassia fistula on the glucose oxidation and molecular mechanisms of glucose up take on streptozotocin-induced diabetic Wistar rats. Phytomedicine 2010; 17: 28-36.
Selvan VT, Mani Kandan L, Senthil Kumar GP, Suresh R, Kumar DA, Mazumdar UK. Antidiabetic and antioxidant effect of methanol extract Artanema Sesamoides in streptozotocin induced diabetic rats. Int J Appl Res Nat Prod 2008; 1(1): 25-33.
Michela CG, Nesseemb DI, Ismailc MF. Anti-diabetic activity and stability study of the formulated leaf extract of Zizyphus spina-christi (L.) Willd with the influence of seasonal variation. J Ethnopharmacol 2011; 133: 53-62.
Kondeti VK, Badri KR, Maddirala DR, Thur SKM, Fatima SS, Kasetti RB, et al. Effect of Pterocarpus santalinus bark, on blood glucose, serum lipids, plasma insulin and hepatic carbohydrate metabolic enzymes in streptozotocin-induced diabetic rats. Food Chem Toxicol 2010; 48: 1281-1287.
Kesari AN, Kesari S, Singh SK, Gupta RK, Watal G. Studies on the glycemic and lipidemic effect of Murraya koenigii in experimental animals. J Ethnopharmacol 2007; 112: 305-311.
Kumar S, Kumar V, Prakash O. Antidiabetic, hypolipidemic and histopathological analysis of Dillenia indica (L.) leaves extract on alloxan induced diabetic rats. Asian Pac J Trop Med 2011; 4(5): 347-352.
Patil R, Patil R, Ahirwar B, Ahirwar D. Isolation and characterization of anti-diabetic component (bioactivity-guided fractionation) from Ocimum sanctum L. (Lamiaceae) aerial part. Asian Pac J Trop Med 2011; 4(4): 278-282.
Patil RN, Patil RY, Ahirwar B, Ahirwar D. Evaluation of antidiabetic and related actions of some Indian medicinal plants in diabetic rats. Asian Pac J Trop Med 2011; 4(1): 20-23.
Kumar S, Kumar V, Prakash O. Antidiabetic and anti-lipemic effects of Cassia siamea leaves extract in streptozotocin induced diabetic rats. Asian Pac J Trop Med 2010; 3(11): 871-873.
Osadebe PO, Omeje EO, Nworu SC, Esimone CO, Uzor PF, David EK, et al. Antidiabetic principles of Loranthus micranthus Linn. parasitic on Persea americana. Asian Pac J Trop Med 2010; 3(8): 619-623.
Kasetti RB, Rajasekhar MD, Kondeti VK, Fatima SS, Kumar EGT, Swapna S, et al. Antihyperglycemic and antihyperlipidemic activities of methanol: water (4:1) fraction isolated from aqueous extract of Syzygium alternifolium seeds in streptozotocin induced diabetic rats. Food Chem Toxicol 2010; 48: 1078-1084.
Eliza J, Daisy P, Ignacimuthu S, Duraipandiyan V. Normoglycemic and hypolipidemic effect of costunolide isolated from Costus speciosus (Koen ex. Retz.) Sm. in streptozotocin-induced diabetic rats. Chem Biol Interact 2009; 179: 329-334.
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