STATISTICAL ANALYSIS OF ACTINOMYCETES ISOLATED FROM THE HARIDWAR REGION OF UTTARAKHAND (INDIA)
Abstract
Statistical analysis of Actinomycetes isolated from biodegraded and non biodegraded building materials at Haridwar region of Uttarakhand (India). The concentrations of viable actinomycetes varied between 50-250 x104 and 5-150x104 cfu/g in the biodegradable and non degradable buildings, respectively. In all studies, the concentrations of viable actinomycetes were higher in the index (biodegradable) buildings than in the reference (non biodegradable) buildings. Phylogenetic characterization of the isolates based on 16S rRNA gene sequencing supported their assignment to 18 different actinomycete genera representing seven different suborders. The result exhibited similarity 91% with 10 strains from the uncultured known species of Streptomyces, Nocardia and Micromonospora. The 16S rRNA nucleotide sequence of Streptomyces, Nocardia and Micromonospora consisted of 749 bp each and the G-C content was 70%. These results show a high diversity of actinomycetes associated with buiding materials and the detection and identification of this group of organisms is extremely important for the deteriogenic process and the development of control methods.
Keywords: Actinomycetes; Nocardia, Micromonospora, building materials, statistical phylogenetic analysis
DOI
https://doi.org/10.22270/jddt.v6i4.1260Published
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