Total Phenolic Contents and Antioxidant Capacity of Aqueous Extract from Pituranthos scoparius (Coss. & Dur.) Growing in Algeria

  • Ahlem Karbab Laboratory of Applied Biochemistry, Department of Biochemistry, Faculty of Natural and Life Sciences, University Ferhat Abbas, Setif-1, 19000 Algeria
  • Kamel Mokhnache Laboratory of Applied Biochemistry, Department of Biochemistry, Faculty of Natural and Life Sciences, University Ferhat Abbas, Setif-1, 19000 Algeria
  • Lekhmici Arrar Laboratory of Applied Biochemistry, Department of Biochemistry, Faculty of Natural and Life Sciences, University Ferhat Abbas, Setif-1, 19000 Algeria
  • Noureddine Charef Laboratory of Applied Biochemistry, Department of Biochemistry, Faculty of Natural and Life Sciences, University Ferhat Abbas, Setif-1, 19000 Algeria

Abstract

This study aims to estimate the total phenolics, flavonoids and tannins contents then to investigate both in vitro antioxidant capacity models of aqueous extract (AqE) from the stems of Pituranthos scoparius, growing in Algeria. Total polyphenol contents were determined using Folin Ciocalteu's reagent; flavonoids were quantified employing the AlCl3 and method tannins using haemoglobin precipitation test. The in vitro antioxidant property was assessed by DPPH-scavenging radical and lipid peroxidation assays. The results revealed that aqueous extract presented a high total phenolic and tannins contents with values of 150.89 ± 0.68 mg GAE (gallic acid equivalent)/g and 71.24 ± 0.09 mg TAE (tannic acid equivalent)/g dry extract, respectively. This extract show an essential effect toward DPPH-scavenging assay and lipid peroxidation inhibition with 96.19 ± 0.00 µg/mL, 91.53 ± 0.98 %, respectively. This study indicates that the aqueous extract from Pituranthos sciparius has potent antioxidant effects and may prove to be of latent health benefit as well as supplementary sources for natural antioxidants drugs.


Keywords: Pituranthos scoparius, aqueous extract, antioxidant activity, phenolic compounds.

Keywords: Pituranthos scoparius, antioxidant activity, phenolic compounds

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Author Biographies

Ahlem Karbab, Laboratory of Applied Biochemistry, Department of Biochemistry, Faculty of Natural and Life Sciences, University Ferhat Abbas, Setif-1, 19000 Algeria

Laboratory of Applied Biochemistry, Department of Biochemistry, Faculty of Natural and Life Sciences, University Ferhat Abbas, Setif-1, 19000 Algeria

Kamel Mokhnache, Laboratory of Applied Biochemistry, Department of Biochemistry, Faculty of Natural and Life Sciences, University Ferhat Abbas, Setif-1, 19000 Algeria

Laboratory of Applied Biochemistry, Department of Biochemistry, Faculty of Natural and Life Sciences, University Ferhat Abbas, Setif-1, 19000 Algeria

Lekhmici Arrar, Laboratory of Applied Biochemistry, Department of Biochemistry, Faculty of Natural and Life Sciences, University Ferhat Abbas, Setif-1, 19000 Algeria

Laboratory of Applied Biochemistry, Department of Biochemistry, Faculty of Natural and Life Sciences, University Ferhat Abbas, Setif-1, 19000 Algeria

Noureddine Charef, Laboratory of Applied Biochemistry, Department of Biochemistry, Faculty of Natural and Life Sciences, University Ferhat Abbas, Setif-1, 19000 Algeria

Laboratory of Applied Biochemistry, Department of Biochemistry, Faculty of Natural and Life Sciences, University Ferhat Abbas, Setif-1, 19000 Algeria

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Karbab A, Mokhnache K, Arrar L, Charef N. Total Phenolic Contents and Antioxidant Capacity of Aqueous Extract from Pituranthos scoparius (Coss. & Dur.) Growing in Algeria. JDDT [Internet]. 15May2020 [cited 1Jun.2020];10(3):125-7. Available from: http://jddtonline.info/index.php/jddt/article/view/4096