QUANTITATIVE DETERMINATION AND OPTIMIZATION OF EXTRACTION CONDITIONS FOR EMBELIN IN EMBELIA SCHIMPERI BY UV-VIS SPECTROMETRY

  • Yehualashet Belete Ethiopian Public Health Institute (EPHI), Traditional and Modern Medicine Research Directorate, Addis Ababa, Ethiopia.
  • Yared Debebe Ethiopian Public Health Institute (EPHI), Traditional and Modern Medicine Research Directorate, Addis Ababa, Ethiopia.
  • Abiy Abebe Ethiopian Public Health Institute (EPHI), Traditional and Modern Medicine Research Directorate, Addis Ababa, Ethiopia.
  • Temesgen Menberu Ethiopian Public Health Institute (EPHI), Traditional and Modern Medicine Research Directorate, Addis Ababa, Ethiopia.
  • Asfaw Debella Ethiopian Public Health Institute (EPHI), Traditional and Modern Medicine Research Directorate, Addis Ababa, Ethiopia.

Abstract

Embelin is a unique chemical compound found in nature, composed of quinone moiety resembling Coq10 (Ubiquinones), having ketone and hydroxy groups with an aliphatic chain. An Optimum solvent for extracting Embelin from Embelia schimperi and its simultaneous determination of the content of Embelin in the extract has been performed using UV-VIS spectrometry. The identification of Embelin spot obtained from hot ethyl acetate extract of the seed is confirmed by Nuclear Magnetic Spectroscopy (NMR). A plot of peak absorbance versus concentration of Embelin was found to be linear over the range of 3-12 μg/ml. The limit of detection was 0.11μg/ml and the limit of quantitation was 0.37 μg/ml. Extraction conditions were also optimized for the best possible extraction of Embelin from the fruits of Embelia schimperi in different solvent polarity like n-hexane, carbon tetrachloride, diethyl ether, chloroform, ethyl acetate, propanol and ethanol. The determination of Embelin in various solvent extract exhibited a mean content of 0.66-5.79 % w/w. Carbon tetrachloride and ethyl acetate was found to be best for the highest possible recovery of the analyte, Embelin. The developed UV method was validated in terms of precision, accuracy, stability, LOD and LOQ

Keywords: Embelia schimperi, Embelin, spectrometry, extraction

Downloads

Download data is not yet available.
Statistics
290 Views | 341 Downloads
How to Cite
1.
Belete Y, Debebe Y, Abebe A, Menberu T, Debella A. QUANTITATIVE DETERMINATION AND OPTIMIZATION OF EXTRACTION CONDITIONS FOR EMBELIN IN EMBELIA SCHIMPERI BY UV-VIS SPECTROMETRY. JDDT [Internet]. 14Jul.2014 [cited 18Sep.2021];4(4):10-3. Available from: http://jddtonline.info/index.php/jddt/article/view/924