Granulocyte colony stimulating factor (GCSF) protected in ovarian tissues against ischemia-reperfusion injury

  • Ayfer Aktaş 1 Department of Histology and Embryology, Faculty of Medicine, Dicle University, Diyarbakır, Turkey
  • Fırat Aşır Department of Histology and Embryology, Faculty of Medicine, Dicle University, Diyarbakır, Turkey
  • Süreyya Özdemir Başaran 1 Department of Histology and Embryology, Faculty of Medicine, Dicle University, Diyarbakır, Turkey
  • Özge Kaplan 1 Department of Histology and Embryology, Faculty of Medicine, Dicle University, Diyarbakır, Turkey
  • Işılay Sezen Ermiş 1 Department of Histology and Embryology, Faculty of Medicine, Dicle University, Diyarbakır, Turkey
  • Engin Deveci 1 Department of Histology and Embryology, Faculty of Medicine, Dicle University, Diyarbakır, Turkey
DOI https://doi.org/10.22270/jddt.v12i4.5538

Abstract

Ovarian torsion is an important reproductive gynecological emergency in daily life and physiology. Granulocyte colony stimulating factor (G-CSF) is a glycoprotein capable of hematopoietic development and successfully used in the treatment of congenital granulocytopenia. In our study, we aimed to investigate protective effects of G-CSF on ovarian ischemia reperfusion (IR) immunohistochemically. Four groups were used. In control group, abdomen was opened and closed with the surgical protocol. The rats in the G-CSF group were given 100 µg/kg G-CSF subcutaneously before the IR and the abdomen was surgically opened and closed. 3-h of ischemia was and then 3-h reperfusion was induced in the ischemia-reperfusion (I/R) group. In the IR+G-CSF group, 100 µg/kg G-CSF was given before the procedure and IR was performed. At the end of the experiment, ovarian tissues were fixed in 10% formalin and then processed for routine paraffin tissue protocol. Normal ovarian histology was observed in the control and G-CSF groups. In the IR group, vascular dilatations, hemorrhage and increased inflammation were observed. In the I/R+G-CSF group, pathology in seen IR was decreased. IL-6 expression was mainly negative in control and G-CSF groups. Positive IL-6 immune reaction was observed in the granulosa cells and stromal area. In the I/R+G-CSF group, IL-6 expression was significantly decreased in ovarian follicular structures and in the stromal area compared to the I/R group. In conclusion, G-CSF reduced vascular dilatation and inflammation in the ovarian IR model and promoted ovarian folliculogenesis.


Keywords: Ovary, Ischemia/reperfusion, G-CSF, Azan, IL-6, Immunohistochemistry

Keywords: ovary, ischemia/reperfusion, G-CSF, Azan, IL-6, immunohistochemistry

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Author Biographies

Ayfer Aktaş, 1 Department of Histology and Embryology, Faculty of Medicine, Dicle University, Diyarbakır, Turkey

1 Department of Histology and Embryology, Faculty of Medicine, Dicle University, Diyarbakır, Turkey

Fırat Aşır, Department of Histology and Embryology, Faculty of Medicine, Dicle University, Diyarbakır, Turkey

Department of Histology and Embryology, Faculty of Medicine, Dicle University, Diyarbakır, Turkey

Süreyya Özdemir Başaran, 1 Department of Histology and Embryology, Faculty of Medicine, Dicle University, Diyarbakır, Turkey

1 Department of Histology and Embryology, Faculty of Medicine, Dicle University, Diyarbakır, Turkey

Özge Kaplan, 1 Department of Histology and Embryology, Faculty of Medicine, Dicle University, Diyarbakır, Turkey

1 Department of Histology and Embryology, Faculty of Medicine, Dicle University, Diyarbakır, Turkey

Işılay Sezen Ermiş, 1 Department of Histology and Embryology, Faculty of Medicine, Dicle University, Diyarbakır, Turkey

1 Department of Histology and Embryology, Faculty of Medicine, Dicle University, Diyarbakır, Turkey

Engin Deveci, 1 Department of Histology and Embryology, Faculty of Medicine, Dicle University, Diyarbakır, Turkey

1 Department of Histology and Embryology, Faculty of Medicine, Dicle University, Diyarbakır, Turkey

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1.
Aktaş A, Aşır F, Başaran S Özdemir, Kaplan Özge, Ermiş IS, Deveci E. Granulocyte colony stimulating factor (GCSF) protected in ovarian tissues against ischemia-reperfusion injury. JDDT [Internet]. 15Jul.2022 [cited 12May2024];12(4):26-0. Available from: https://jddtonline.info/index.php/jddt/article/view/5538
Issue
Vol 12 No 4 (2022): Volume 12, Issue 4, July-August 2022
Section
Research
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