STABILITY INDICATING LIQUID CHROMATOGRAPHIC METHOD FOR THE QUANTITATIVE DETERMINATION OF VALGANCICLOVIR IN PHARMACEUTICAL DOSAGE FORMS
A selective, specific and sensitive stability-indicating high-performance liquid chromatographic method was developed and validated for the determination of Valganciclovir in tablet dosage forms. Reversed-phase chromatography was performed on Shimadzu Model CBM-20A/20 Alite, equipped with SPD M20A prominence photodiode array detector (Isocratic mode) using C18 column (250 mm Ã— 4.6 mm, 5 Î¼m) with a flow rate of 0.8 mL/min.Â UV detection was carried at 254 nm.Â Linearity was observed in the concentration range of 1.0â€“200 Î¼g/mL with regression equation y = 50968 x + 86374 with correlation coefficient of 0.999. The LOQ and LOD were found to be 0.8641 Î¼g/mL and 0.2813 Î¼g/mL respectively. Valganciclovir was subjected to stress conditions such as acidic, alkaline, oxidation, photolysis and thermal degradations. The developed method was validated as per ICH guidelines and it can be applied for the determination of Valganciclovir in pharmaceutical dosage forms.
Keywords: Valganciclovir, Isocratic mode, RP-HPLC, Validation, Stability-indicating, LOD, LOQ.
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