Acute Toxicity Study of Anvillea Radiata Aqueous Extract in Albino Rats
Despite the popular use and the biological effects of Anvillea radiata, there are no studies or data about its safety. The aim of the present study was to assess the acute toxicity of A. radiata aqueous extract in vivo. A single dose of 0.25, 0.5, 1, 1.5, 2.5 or 5 g/kg was administered to female rats by gavage. Body weight gain, general behavior and mortality were monitored for up to 2 weeks. Selected biochemical parameters, aspartate aminotransferase (AST), alanine aminotransferase (ALT), blood urea nitrogen (BUN) and blood creatinine levels were determined, as well as, liver and kidney histology. Results showed no significant changes in body weight gain and organ indexes with no mortality during the experimentation period. A significant increase in AST and ALT levels were observed in 2.5 and 5 g/kg extract treated groups, and a significant decrease in BUN and creatinine levels in 1, 1.5, 2.5 or 5g/kg extract treated groups compared to control. Microscope examination of liver sections showed several anomalies in rats exposed to high concentrations (1.5, 2.5 and 5 g/kg) including fatty changes, glycogen accumulation and ballooning degeneration hepatocytes. Renal parenchyma anomalies were also observed in rats exposed to 2.5 and 5g / kg of plant extract including shrunken renal corpuscles with marked hypo-cellularity and atrophied glomeruli, large interstitial space, and renal tubules with dilated lumina which appear completely distorted. From this study, it can be concluded that Anvillea radiata aqueous extract at high concentration (higher than 1 g /kg b.w.) may be toxic and affect sensitive organs function such liver and kidney.
Keywords: Anvillea radiata, Acute toxicity, Biochemical parameters, Histology.
2. Boukhris M.A., et al., A dereplication strategy for the identification of new phenolic compounds from Anvillea radiata (Coss. & Durieu). Comptes Rendus Chimie, 2016; 19(9):1124-1132.
3. Kandouli C., et al., Antidiabetic, antioxidant and anti inflammatory properties of water and n-butanol soluble extracts from Saharian Anvillea radiata in high-fat-diet fed mice. Journal of ethnopharmacology, 2017; 207:251-267.
4. Lakhdar M., et al., Phytochemical analysis and antifungal activity of Anvillea radiata. World Applied Sciences Journal, 2013; 26(2):165-171.
5. Abdel Sattar E., A.M. Galal, and G.S. Mossa, Antitumor germacranolides from Anvillea garcinii. Journal of natural products, 1996; 59(4):403-405.
6. Boudjerda A., et al., A new guaianolide and other constituents from Achillea ligustica. Biochemical Systematics and Ecology, 2008; 36(5-6):461-466.
7. Havsteen B.H., The biochemistry and medical significance of the flavonoids. Pharmacology & therapeutics, 2002; 96(2-3):67-202.
8. Serairi‐Beji R., et al., Antioxidant and hepatoprotective effects of Asparagus albus leaves in carbon tetrachloride‐induced liver injury rats. Journal of Food Biochemistry, 2018; 42(1):e12433.
9. Kim E.-J., et al., An improved dehydroepiandrosterone-induced rat model of polycystic ovary syndrome (PCOS): Post-pubertal improve PCOS's features. Frontiers in Endocrinology, 2018; 9:735.
10. Qin D., et al., Effect of cichorium glandulosum extracts on CCl4-induced hepatic fibrosis. Iranian Red Crescent Medical Journal, 2013. 15(12).
11. Boudou F., et al., Ameliorative effects of Syzygium aromaticum essential oil on fertility in male rats exposed to manganese. Advances in sexual medicine, 2013; 2013.
12. Lee M.-Y., et al., Subchronic oral toxicity studies of the traditional herbal formula Bangpungtongseong-san in Crl: CD (SD) rats. Journal of ethnopharmacology, 2012; 144(3):720-725.
13. Babulka P., Les rumex, de l’ethnobotanique à la phytothérapie moderne (Rumex spp.). Phytothérapie, 2004; 2(5):153-156.
14. Araújo M.C.d.P.M., et al., Acute and sub chronic toxicity study of aqueous extract from the leaves and branches of Campomanesia velutina (Cambess) O. Berg. Journal of ethnopharmacology, 2017; 201:17-25.
15. Perveen S., et al., Hepatoprotective and cytotoxic activities of Anvillea garcinii and isolation of four new secondary metabolites. Journal of natural medicines, 2018; 72(1):106-117.
16. Burtis C.A. and D.E. Bruns, Tietz fundamentals of clinical chemistry and molecular diagnostics-e-book. 2014: Elsevier Health Sciences.
17. Benslama A., et al., In vitro Antioxidant, Antibacterial Activities and HPLC-TOF/MS Analysis of Anvillea radiata (Asteraceae) Extracts. Current Nutrition & Food Science, 2019; 15(4):376-383.
18. Kandouli C., et al. In vitro and in vivo evaluation of the antioxidant, antidiabetic and cardioprotective activities from the aerial parts of Anvillea radiata Coss. & Dur. in 15th International Conference on Oxidative Stress Reduction, Redox Homeostasis and Antioxidants. 2015.
19. Hebi M. and M. Eddouks, Glucose lowering activity of anvillea radiata coss & durieu in diabetic rats. Cardiovascular & Haematological Disorders-Drug Targets (Formerly Current Drug Targets-Cardiovascular & Hematological Disorders), 2018; 18(1):71-80.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).