Evaluation of Antioxidant Activity and Polyphenols Content of the Hydro-methanolic Extract from Saccocalyx satureioides Coss and Dur

  • Soulaf MEHLOUS Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria
  • Fatima BENCHIKH Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria
  • Hassiba BENABDALLAH Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria
  • Karima LOUCIF Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria
  • Chahrazed KAOUDOUNE Laboratory of Environmental Biosurveillance, Faculty of Sciences, Department of Biology, BadjiMokhtar University, 23000-Annaba, Algeria
  • Hocine LAOUER Laboratory of Valorisation of Natural Biological Resources, Department of Biology, University of Setif1, Algeria
  • Smain AMIRA Laboratory of Valorisation of Natural Biological Resources, Department of Biology, University of Setif1, Algeria

Abstract

This study aimed to estimate the total phenolic and flavonoid contents and to evaluate the antioxidant activity of the Hydro-methanolic from Saccocalyx satureioides Coss and Dur aerial part. Total polyphenols contents were determined using Folin-Ciocalteu’s reagent. The flavonoids were estimated using the method of Aluminum chloride (AlCl3). The antioxidant capacity was evaluated using two in vitro models (the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and reducing power). Total phenolic and flavonoid content in the extract were 171.34 ±1.43 mg Gallic acid equivalent/g of dry extract (GAE/g) and 18.6 ± 0.46 mg Quercetin equivalent / g of dry extract (QE/g), respectively. The methanol extract has an important capacity to scavenge the free radical DPPH with an IC50 of 0.03 ± 0. 0024 mg/ml. In addition, the plant extract exhibited high reducing power with an IC50 of 0.54 ± 0.00625 mg/ml. The results of the present study may prove that the medicinal plants are a good resource of natural antioxidants.


Keywords: Antioxidant activity, Saccocalyx satureioides, polyphenols, flavonoids.

Keywords: Antioxidant activity, Saccocalyx satureioides, polyphenols, flavonoids

Downloads

Download data is not yet available.

Author Biographies

Soulaf MEHLOUS, Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria

Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria

Fatima BENCHIKH, Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria

Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria

Hassiba BENABDALLAH, Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria

Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria

Karima LOUCIF, Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria

Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria

Chahrazed KAOUDOUNE, Laboratory of Environmental Biosurveillance, Faculty of Sciences, Department of Biology, BadjiMokhtar University, 23000-Annaba, Algeria

Laboratory of Environmental Biosurveillance, Faculty of Sciences, Department of Biology, BadjiMokhtar University, 23000-Annaba, Algeria

Hocine LAOUER, Laboratory of Valorisation of Natural Biological Resources, Department of Biology, University of Setif1, Algeria

Laboratory of Valorisation of Natural Biological Resources, Department of Biology, University of Setif1, Algeria

Smain AMIRA, Laboratory of Valorisation of Natural Biological Resources, Department of Biology, University of Setif1, Algeria

Laboratory of Valorisation of Natural Biological Resources, Department of Biology, University of Setif1, Algeria

References

1. Belmeki N, Bendimerad N. Antioxidant activity and phenolic content in methanol crudeextracts from three Lamiaceae grown in southwesternAlgeria. J Nat Prod Plant Resour 2012, 2 (1):175-181.
2. Dian M S, Trilianty L, Fransisca D L, Helena J, Iskandar T. Antioxidant and Anti-Glycation Activity of Ethanol Lemongrass (Cymbopogon citratus) Leaves Extract. International Journal of Pharmacognosy and Phytochemical Research, 2017; 9(5):710-715.
3. Amira S, Dade M, Schinella G, Rios JL. Anti-inflammatory, antioxidant and apoptotic activities of four plant species used in folk medicine in the Mediterraneanbasin. Pak J Pharmaceut Sci. 2012, 25:65-72.
4. Clarice DCV, Mayara B D, Marianne D OM, Marcelo H D S, Alexandre G-P, Fabiana C V. Evaluation of anxiolytic-like effect of 7-epiclusianone isolated from Garcinia brasiliensis in mice. Revista Brasileira de Farmacognosia, 2018;28:378–381.
5. Quezel P, Santa S. Nouvelle flore de l'Algérie et des régions désertiques méridionales. Centre National de la Recherche Scientifique, Paris. 1963.
6. Kherkhache H, Benabdelaziz I, Silva A M S, Lahrech M B, BenaliaM, Haba H. A new indole alkaloid, antioxidant and antibacterial activities of crude extracts from Saccocalyx satureioides. Natural Product Research, 2018; 34(11):1528-1534.
7. Benahmed M, Djeddi N, Akkal S, Laouar H. Saccocalyx satureioides as corrosion inhibitor for carbon steel in acid solution. Int J Ind Chem, 2016; 7: 109–120.
8. Li HB, Cheng KW, Wong CC, Fan KW, Chen F, Jiang Y. Evaluation of antioxidant capacity and total phenolic content of different fractions of selected microalgae. Food Chem, 2007; 102:771-776.
9. Bahorun T, Gressier B, Trotin F, Brunete C, Dine T, Vasseur J, Luyckx M, Cazin M, Pinkas M. Oxygen species scavenging activity of phenolic extract from Hawthorn fresh plant organs and pharmaceutical preparations. Arzneim Forsch/Drug Res, 1996; 46: 1086-1089.
10. Blois MS. Antioxidant determinations by the use of a stable free radical. Nature, 1958; 181:1199-1200.
11. Burits M, Bucar F. Antioxidant activity of Nigella sativa essential oil. Phytother Res, 2000; 14:323-28.
12. Chung YC, Chen SJ, Hsu CK, Chang CT, Chou ST. Studies on the antioxidative activity of Graptopetalum paraguayense. Food Chemistry, 2005; 91:419-424.
13. Benchikh F, Amira S, Benabdallah, H. The evaluation of antioxidant capacity of different fractions of Myrtus communis L. leaves. Annual Research & Review in Biology, 2018; 22(5):1-14.
Statistics
4 Views | 9 Downloads
How to Cite
1.
MEHLOUS S, BENCHIKH F, BENABDALLAH H, LOUCIF K, KAOUDOUNE C, LAOUER H, AMIRA S. Evaluation of Antioxidant Activity and Polyphenols Content of the Hydro-methanolic Extract from Saccocalyx satureioides Coss and Dur. JDDT [Internet]. 15Jul.2020 [cited 4Aug.2020];10(4):188-90. Available from: http://jddtonline.info/index.php/jddt/article/view/4166