Evaluation of Hepatoprotective Activity of Hydroalcoholic Extract of Onion Peels Containing Protocatechuic Acid
India is the second largest country for cultivation of onion. 19.90% of onion is cultivated in India. Onion that is Allium cepa family Amaryllidaceae used in daily diet for taste. Onions also have number of medicinal properties, such as hepatoprotection, anticancer, antifungal, antioxidant, antiulcer, anti-agging, anti-inflammatory, anticancer etc.[1-2] The dried scales or peels of onion also have the same medicinal constituents with same activity as raw onions. The onion peels contains flavonoids, such as anthocyanins, flavones (qurcertin and its derivatives), ferulic, Gallic, protocatechuic acids, sulphur, vitamins etc. In the present study determination of protocatechuic acid in onion peels extract has been performed using HPTLC. HPTLC separation was carried out on Merck TLC aluminium sheets precoated with silica gel 60F254 using Toluene: Ethyl acetate: Formic acid (6: 6: 1.2 v/v/v) as mobile phase.[3-11] Quantitative analysis was carried out in the absorbance mode at 258 nm. Hydroalcoholic extract was tested for hepatoprotective activity in wistar rats (either sex) by using CCl4 as hepatotoxicity inducing agent and Silamyrin as standard. Hydroalcoholic extract shows hepatoprotective activity as indicated by decrease in the level of SGOT, SGPT, total protein, billirubin an which hepatotoxicity was induced by CCl4 intraperitonial injection route to animal, from the result it may be concluded that onion peels extract may be used for hepatoprotective activity.
Keywords: Onion peel extract, Protocatechuic acid, HPTLC, hepatoprotection.
2. https://www.drugbank.ca/drugs/DB03946 (Accessed on 13-07-2018).
3. Sahil Kakkar and Souravh Bais, A Review on Protocatechuic Acid and Its Pharmacological Potential, International scholarly Research Notices Pharmacology journal, Volume 2014, page no. 1-9.
4. Manek Ravi , Patel N. M.2, Determination of Protocatechuic Acid by HPTLC Method in Amomum subulatum Roxb. Fruit Extracts PHCOG Journal, Augest 2009, page no. 130 to 137.
5. Abida kalsoom khan1, pharmacological activities of protocatechuic acid, Acta Poloniae Pharmaceutica ñ Drug Research, 2015,Vol. 72 No. 4 pp. 643-650.
6. Pandey Shivanand, R. Mahalaxmi, Identification and determination of protocatechuic acid present in greater cardamom fruit extracts by HPTLC technique, International Journal of Pharmaceutical Sciences Review and Research Volume 1, issue 1, page no. 27 to 32.
7. Rana Arslan, The possible mechanisms of protocatechuic acid induced central analgesia, Saudi pharmaceutical journal 2018, page no. 541-545.
8. Brahma N. Singh polyphenolics from various extract/fraction of red onion (Allium Cepa) peel with potent antioxidant and amtimutagenic activity, journal of Food and Chemical Toxicology 47 (2009) 1161–1167.
9. Jungmi Kirn cytotoxic and anti-inflammatory effect ofonion peel extract on lipopolysaccharide stimulated human colon carcinoma cells, journal of food and chemical toxicology 2003, page no. 199-204.
10. Srinivasan, K. Plant foods in the management of diabetes mellitus: spices as beneficial antidiabetic food adjuncts. Int. J. Food Sci. Nutr.2005;56(6):399-414. 16361181
11. Ozougwu, Jevas C, Physiology of the liver, International Journal of Research in Pharmacy and Biosciences Volume 4, Issue 8, 2017, PP 13-24
12. http://www.berkem.com/en/expertise-en/plant-extraction (Accessed on 22 April 19)
13. http://agritech.tnau.ac.in/horticulture/extraction_techniques%20_medicinal_plants.pdf (Accessed on 22 April 19)
14. https://www.webmd.com/digestive-disorders/picture-of-the-liver#1 (Accessed on 22 April 19)
15. Salami H.A, John A.I. et al., The effect of aqueous preparation of Allium Cepa (onion) and Allium Sativa (garlic) on erythrocyte osmotic fragility in wistar rats: in vivo and in vitro studies, Nigerian journal of Physiological Science, volume 27. June 2012, page no. 029-034.
16. Butura A. Drug and Alcohol Induced Hepatotoxicity. 2008 Ph. D Thesis Department of Physiology and Pharmacology Karolinska Institutet, Stockholom, Sweden. 55 pp.
17. Kmiec Z. Co-operation of liver cells in health and diseases. Advances in Anatomy, Embryology and Cell Biology 2001, 161: 3 – 12.
18. Rang and dales book of pharmacology, 8th edition 2016, page no. 456-460
19. Kavita Gulati, Mohd Rafi Reshi et al., Hepatotoxicity: Its Mechanisms, Experimental Evaluation and Protective Strategies, American Journal of Pharmacology, 2018; 1(1): 1004(article no)
20. RakeshSagar, Amrita Bhaiji, et al., A comprehensive review on herbal drugs for hepatoprotection of 21st Century, International Journal of Nutrition, Pharmacology, Neurological Diseases | October-December 2014 | Vol 4| Issue 4, pg no 191-197
21. G. Dineshkumar hepatoprotective activity of leaves extract of eichhornia crassipes against ccl4 induced hepatotoxicity albino rats, International Journal of Pure and Applied Zoology, Vol. 1, Issue 3, pp: 209-212, 2013.
22. Prashant Tiwari Evaluation of hepatoprotective activity of alcoholic and aqueous extracts of Selaginella lepidophylla, Asian Pacific Journal of Tropical Disease,page nor. S81 to S86.
23. Sumanta Mondala, Hepatoprotective activity of Macrothelypteris torresiana (Gaudich.) aerial parts against CCl4-induced hepatotoxicity in rodents and analysis of polyphenolic compounds by HPTLC, Journal of Pharmaceutical Analysis, page no. 181-189.
24. ZHANG Zhi-Feng, Hepatoprotective activity of Gentiana veitchiorum Hemsl. against carbon tetrachloride-induced hepatotoxicity in mice, Chinese Journal of Natural Medicines 2014, section 12(7) page no.0488-0494.
25. Kalpana Patil, Hepatoprotective activity of Mentha arvensis Linn. leaves against CCl4 induced liver damage in rats, Asian Pacific Journal of Tropical Disease, page no. 5223-5226
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