Protective effects of some Generally Recognized As Safe (GRAS) grade food preservatives against experimentally induced renal dysfunction
Drug induced nephrotoxicity is the current concern of research due to its awful worldwide occurrences. Generally recognized as safe (GRAS) grade food preservatives e.g. butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), L-ascorbic acid (Vit.C) and gamma-tocopherol (Vit. E) exhibits potent antioxidant, anti-inflammatory properties against severe oxidative stress. The aim of this study was to evaluate the efficacy of food preservatives on carbon tetrachloride (CCl4)-induced (230 mg/ kg b wt/ rat/day) nephritic damage in rats. Nephritic markers like serum urea, blood urea nitrogen, serum creatinine; antioxidant markers such as GSH, SOD, CAT, GPx, and lipid peroxidation end product, MDA were measured to establish anti-oxidant properties of said food preservatives and vitamins. The results had shown an elevated level of serum urea (387.30%), blood urea nitrogen (376%), serum creatinine (646.82%) and marked decreased activity of antioxidant markers like SOD (81.03%), CAT (72.24%), GSH (63.04%), GPx (50.34%) as well. CCl4 induced nephrotoxicity also caused 48.14% and 59.47% increase in sodium and potassium concentration. Histological studies also confirmed that antioxidant status in renal cells was restored as BHA, BHT, L-ascorbic acid, and gamma-tocopherol successfully ameliorated certain degenerative changes caused due to CCl4 intoxication. Therefore, it can be concluded that supplementation of certain food preservatives like BHA, BHT and like Vitamins L-ascorbic acid, gamma-tocopherol may be potentially beneficial to the community affected by severe renal dysfunction.
Keywords: butylated hydroxyanisole, butylated hydroxytoluene, L-ascorbic acid, gamma-tocopherol, CCl4 intoxication.
(2) Rossberg M, Gerhartz W, Yamamoto YS, Campbell FT. 5th edition. Chlorinated hydrocarbons, Ullmann’s encyclopedia of industrial chemistry, VCH Publishers, New York. 2002;370
(3) Dassarma B, Samanta S, Nandi DK. Toxicokinetics of carbontetrachloride (CCl4) exposure on hepatorenal system. Euro. J. Biomed. Pharmace. Sci. 2017a; 4(1):520-5.
(4) Bahcecioglu, I.H., Ustundag, B., Ozercan, I., Ercel, E., Baydas, G.,Akdere, T., Demir, A., Protective effect of Ginkgo biloba extracton CCI4-induced liver damage. Hepatology Research 1999; 15:215–224.
(5) Fadhel, Z.A., Amran, S., Effects of black tea extract on carbon tetrachloride-induced lipid peroxidation in liver, kidneys, and testes of rats. Phytotherapy Research 2002; 16:28–32.
(6) Kahl R, Kappus H. Toxicology of the synthetic antioxidants BHA and BHT in comparison with the natural antioxidant vitamin E. Zeitschrift fur Lebensmittel-untersuchung und-forschung. 1993; 196(4):329-38.
(7) Yehye WA, Rahman NA, Ariffin A, Hamid SB, Alhadi AA, Kadir FA, Yaeghoobi M. Understanding the chemistry behind the antioxidant activities of butylated hydroxytoluene (BHT): A review. European journal of medicinal chemistry. 2015; 101:295-312.
(8) Burton G, Ingold KU. Autoxidation of biological molecules. 1. Antioxidant activity of vitamin E and related chain-breaking phenolic antioxidants in vitro. Journal of the American Chemical Society. 1981; 103(21):6472-7.
(9) Elgazar AF. Effects of butylated hydroxytoluene and butylated hydroxyanisole against hepatotoxicity induced by carbon tetrachloride in rats. World Applied Sci J. 2013; 22(1):63-9.
(10) Landi M, Degl'Innocenti E, Guglielminetti L, Guidi L. Role of ascorbic acid in the inhibition of polyphenol oxidase and the prevention of browning in different browning‐sensitive Lactuca sativa var. capitata (L.) and Eruca sativa (Mill.) stored as fresh‐cut produce. Journal of the Science of Food and Agriculture. 2013; 93(8):1814-9.
(11) Institute of Medicine (2000). "Vitamin E". Dietary Reference Intakes for Vitamin C, Vitamin E, Selenium, and Carotenoids. Washington, DC: The National Academies Press. pp. 186– 283.
(12) Galli F, Azzi A, Birringer M, Cook-Mills JM, Eggersdorfer M, Frank J, Cruciani G, Lorkowski S, Özer NK. Vitamin E: Emerging aspects and new directions. Free Radical Biology and Medicine. 2017; 102:16-36.
(13) Dalton L. Food Preservatives. Chemical and Engineering News. 2002; 80(45):40.
(14) USDA Food Composition Databases.United States Department of Agriculture, Agricultural Research Service. Release 28. 2015. Retrieved 18 August 2018.
(16) Olfert ED, Cross BM, McWilliam AA, editors. Guide to the care and use of experimental animals. Ottawa: Canadian Council on Animal Care; 1993.
(17) Dassarma B, Samanta S, Nandi D K, Gangopadhyay S. Carbon tetrachloride induced hepatorenal failure in male albino rats: A dose response study. International Journal of Recent Scientific Research. 2017b; 8(3): 15885-15892.
(18) Dassarma B, Nandi DK, Gangopadhyay S, Samanta S. Hepatoprotective effect of food preservatives (butylated hydroxyanisole, butylated hydroxytoluene) on carbon tetrachloride-induced hepatotoxicity in rat. Toxicology reports. 2018a; 5:31-7.
(19) Al-Attar AM. Vitamin E attenuates liver injury induced by exposure to lead, mercury, cadmium and copper in albino mice. Saudi journal of biological sciences. 2011 Oct 1;18(4):395-401.
(20) Asuku O, Atawodi SE, Onyike E. Antioxidant, hepatoprotective, and ameliorative effects of methanolic extract of leaves of Grewia mollis Juss. on carbon tetrachloride–treated albino rats. Journal of medicinal food. 2012; 15(1):83-8.
(21) Coulambe CG, Favrean LA. Quaternary ammonium compound toxicity in chicken. Clinical Chemistry. 1965; 11(17):624.
(22) Bosnes RW, Taussky HH. On the colorimetric determination of creatinine by the Jaffe reaction. J Biol Chem 1945; 158: 581.
(23) Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical biochemistry. 1979; 95(2):351-8.
(24) Maestro RF, McDonald W. Oxidative enzymes in tissue homogenates. Handbook of methods for oxygen radical research. 1985:291-6.
(25) Luck HA. Spectrophotometric method for the estimation of catalase. Methods of enzymatic analysis. 1963.
(26) Moron MS, Depierre JW, Mannervik B. Levels of glutathione, glutathione reductase and glutathione S-transferase activities in rat lung and liver. Biochimica et Biophysica Acta (BBA)-General Subjects. 1979; 582(1):67-78.
(27) Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. The Journal of laboratory and clinical medicine. 1967; 70(1):158-69.
(28) Iranloye BO, Bolarinwa AF. Effect of nicotine administration on weight and histology of some vital visceral organs in female albino rats. Nigerian Journal of Physiological Sciences. 2009; 24(1).
(29) Dassarma B, Jana S, Rakshit S, Nandi D K, Gangopadhyay S, Samanta S. An overview of systemic effects of carbon tetrachloride (CCl4) exposure. Indian Science Cruiser. 2018b; 32(3): 36-40.
(30) Adewole S, Salako A, Doherty O, Naicker T. Effect of melatonin on carbon tetrachloride-induced kidney injury in Wistar rats. African Journal of Biomedical Research. 2007; 10(2).
(31) Rafieian-Kopaei M, Baradaran A. Teucrium polium and kidney. Journal of renal injury prevention. 2013; 2(1):3-4.
(32) Nasri H. On the occasion of the world diabetes day 2013; diabetes education and prevention; a nephrology point of view. Journal of renal injury prevention. 2013;2(2):31.
(33) Pradhan S, Mandal S, Roy S, Mandal A, Das K, Nandi DK. Attenuation of uremia by orally feeding alpha-lipoic acid on acetaminophen induced uremic rats. Saudi Pharmaceutical Journal. 2013; 21(2):187-92.
(34) Ritesh KR, Suganya A, Dileepkumar HV, Rajashekar Y, Shivanandappa T. A single acute hepatotoxic dose of CCl4 causes oxidative stress in the rat brain. Toxicology reports. 2015; 2:891-5.
(35) Tirkey N, Pilkhwal S, Kuhad A, Chopra K. Hesperidin, a citrus bioflavonoid, decreases the oxidative stress produced by carbon tetrachloride in rat liver and kidney. BMC pharmacology. 2005; 5(1):2.
(36) Oyinbo CA, Dare WN, Okogun GR, Anyanwu LC, Ibeabuchi NM, Noronha CC, Okanlawon OA. The hepatoprotective effect of vitamin C and E on hepatotoxicity induced by ethanol in Sprague Dawley rats. Pak J Nutr. 2006; 5(6):507-11.
(37) Sreelatha S, Padma PR. Antioxidant activity and total phenolic content of Moringa oleifera leaves in two stages of maturity. Plant foods for human nutrition. 2009; 64(4):303.
(38) Khan BA, Akhtar N, Rasul A, Mahmood T, Khan HS, Iqbal M, Murtaza G. Investigation of the effects of extraction solvent/technique on the antioxidant activity of Cassia fistula L. Journal of Medicinal Plants Research. 2012; 6(3):500-3.
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