Protective effects of some Generally Recognized As Safe (GRAS) grade food preservatives against experimentally induced renal dysfunction

  • Barsha Dassarma
  • Saptadip Samanta
  • Dilip K Nandi
  • Somnath Gangopadhyay


Drug induced nephrotoxicity is the current concern of research due to its awful worldwide occurrences. Generally recognized as safe (GRAS) grade food preservatives e.g. butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), L-ascorbic acid (Vit.C) and gamma-tocopherol (Vit. E) exhibits potent antioxidant, anti-inflammatory properties against severe oxidative stress. The aim of this study was to evaluate the efficacy of food preservatives on carbon tetrachloride (CCl4)-induced (230 mg/ kg b wt/ rat/day) nephritic damage in rats. Nephritic markers like serum urea, blood urea nitrogen, serum creatinine; antioxidant markers such as GSH, SOD, CAT, GPx, and lipid peroxidation end product, MDA were measured to establish anti-oxidant properties of said food preservatives and vitamins. The results had shown an elevated level of serum urea (387.30%), blood urea nitrogen (376%), serum creatinine (646.82%) and marked decreased activity of antioxidant markers like SOD (81.03%), CAT (72.24%), GSH (63.04%), GPx (50.34%) as well. CCl4 induced nephrotoxicity also caused 48.14% and 59.47% increase in sodium and potassium concentration. Histological studies also confirmed that antioxidant status in renal cells was restored as BHA, BHT, L-ascorbic acid, and gamma-tocopherol successfully ameliorated certain degenerative changes caused due to CCl4 intoxication. Therefore, it can be concluded that supplementation of certain food preservatives like BHA, BHT and like Vitamins L-ascorbic acid, gamma-tocopherol may be potentially beneficial to the community affected by severe renal dysfunction.  

Keywords: butylated hydroxyanisole, butylated hydroxytoluene, L-ascorbic acid, gamma-tocopherol, CCl4 intoxication.


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Dassarma B, Samanta S, Nandi DK, Gangopadhyay S. Protective effects of some Generally Recognized As Safe (GRAS) grade food preservatives against experimentally induced renal dysfunction. JDDT [Internet]. 15Jun.2019 [cited 2Jul.2020];9(3-s):412-9. Available from: