Improved Biological Activity and Stability of enzyme L-Asparaginase in Solid Lipid Nanoparticles Formulation

Controlled release formulation of L-Asparaginase

  • Gazal Sharma PhD Researcher, IKG Punjab Technical University, Kapurthala, Punjab India
  • Goutam Rath Professor, Department of Pharmaceutics, ISF College of Pharmacy Moga, Punjab, India
  • Amit Goyal Scientist E, National Animal Institute of Biotechnology, Hyderabad, Andhra Pradesh India

Abstract

To protect the biological activity of an enzyme during the development of formulations is one of the biggest challenges. The tetrameric form of L-Asparaginase is used to treat Acute Lymphocytic Leukaemia. It possesses shorter in vivo half–life. Using a modified (water/oil)/water-emulsion method followed by solvent evaporation L-Asn was successfully encapsulated at the core of Solid Lipid Nanoparticles made of lipid glyceryl monostearate. This study elucidated that the preparation of L-Asn loaded SLN develop a colloidal formulation with enhanced activity. The in-vitro release profile of the enzyme revealed first bursts has been increased. The study of the lyophilised formulation also shows that the enzyme holds its biological activity and retains its particle size distribution. Consequently, by using an apt combination of homogenisation speed, temperature and additives the storage and biological activity of L-Asn in SLN formulation can be improved.


Keywords: L-Asparaginase, Solid Lipid Nanoparticles, Controlled release, Lymphocytic Leukaemia

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Author Biographies

Gazal Sharma, PhD Researcher, IKG Punjab Technical University, Kapurthala, Punjab India

PhD Researcher, IKG Punjab Technical University, Kapurthala, Punjab India

Goutam Rath, Professor, Department of Pharmaceutics, ISF College of Pharmacy Moga, Punjab, India

Professor, Department of Pharmaceutics, ISF College of Pharmacy Moga, Punjab, India

Amit Goyal, Scientist E, National Animal Institute of Biotechnology, Hyderabad, Andhra Pradesh India

Scientist E, National Animal Institute of Biotechnology, Hyderabad, Andhra Pradesh India

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How to Cite
Sharma, G., Rath, G., & Goyal, A. (2019). Improved Biological Activity and Stability of enzyme L-Asparaginase in Solid Lipid Nanoparticles Formulation. Journal of Drug Delivery and Therapeutics, 9(2-s), 325-329. https://doi.org/10.22270/jddt.v9i2-s.2708