Extraction phytochemical screening and development of phytosomes of hydroalcoholic extract of Terminalia chebula Retz for hepetoprotection
Abstract
Evidences from ethnopharmacological practices have shown that Terminalia chebula Retz are traditionally used to treat symptoms of the liver disorder. In the current days, most of the prevailing diseases and nutritional disorders are treated with natural medicines. The effectiveness of any herbal medication is dependent on the delivery of effective level of the therapeutically active compound. But a severe limitation exists in their bioavailability when administered orally or by topical applications. Phytosomes are recently introduced herbal formulations that are better absorbed and as a result produced better bioavailability and actions than the conventional phyto molecules or botanical extracts. The aim of the present study was to evaluate qualitative and quantitative phytochemical analysis, HPLC, optical microscopic study and in vitro antioxidant activities of leaf of Terminalia chebula Retz collected from Bhopal region of Madhya Pradesh. The Hydroalcoholic extract of phytosome was prepared in phospholipids:cholesterol. Characterization of phytosome was done by FTIR, Entrapment efficiency, Particle size and size distribution, optical microscopic study, HPLC and stability studies, In vitro dissolution studies, and in vitro antioxidant activity by DPPH model. Combination of phospholipids and Terminalia chebula Retz can result in synergistic effect, Synergistic effect measure with free radical scavenging activity use DPPH model.
Keywords: Terminalia chebula Retz, Phytosome, Phospholipids, DPPH model, free radical scavenging activity
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References
2. Kim TY, Kim DJ. Acute-on-chronic liver failure, Clinical and Molecular Hepatology, 2013; 19(4):349–359.
3. Bernal W, Auzinger G, Dhawan A, Wendon JAcute liver failure, The Lancet, 2010; 376(9736):190–201
4. CSIR. The wealth of India - A dictionary of indian raw materials and industrial products. Vol X. New Delhi: Publication and Information Directorate, CSIR; 2002, p. 522-524.
5. Varier. A dictionary of Indian raw materials and industrial products. New Delhi: Publications and Information Directorate, Council of Scientific and Industrial Research; 2002, p. 387.
6. Khare CP. Indian medicinal plants: An illustrated dictionary. Berlin: Springer-Verlag; 2007, p. 652-653
7. Bombardelli E, Spelta M. Phospholipid-polyphenol complex a new concept in skin care ingredients. Cosmet Toiletries. 1991; 106:69–76.
8. Bombardelli E, Mustich G. Bilobalide Phospholipid Complex, their Uses and formulation Containing them. U.S. Patent EPO- 275005. 1991
9. Jaiswal P, Kesharwani S, Kesharwani R, Patel D, Ethosome: A New Technology Used As Topical & Transdermal Delivery System. Journal of Drug Delivery and Therapeutics, 2016; 6(3):7-17.
10. Jaeschke H, Werner C, Wendel A. Disposition and hepatoprotection by phosphatidyl choline liposomes in mouse liver. Chem Biol Interact. 1987; 64:127–37.
11. Canty DJ, Zeisel SH. Lecithin and choline in human health and disease. Nutr Rev. 1994; 52:327–9.
12. Khandelwal KR. Practical pharmacognosy technique and experiments. 23rd Ed. Nirali Prakashan; 2005.
13. Kokate CK. Practical pharmacognosy. 4th Ed. Vallabh Prakashan; 1994.
14. Olufunmiso OO, Afolayan AJ, Phenolic content and antioxidant property of the bark extract of Ziziphus mucronata willd. Subsp. mucronata willd, BMC Complement Alternative Medicine, 2011; 11:130.
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