Two-tailed fancy goldfish challenged with Bacteroides spp. isolated from shubunkin goldfish

  • Alvin T Reyes College of Fisheries-Freshwater Aquaculture Center, Central Luzon State University, Science City of Muñoz, Nueva Ecija, Philippines
DOI https://doi.org/10.22270/jddt.v9i3.2681

Abstract

This study aimed to isolate, morpho-biochemically characterize and identify the most common bacterium in diseased shubunkin goldfish, and challenge the two-tailed fancy goldfish using the identified bacterium.  With the aid of Bergey’s Manual of Determinative Bacteriology, the bacterium was identified as Bacteroides spp. The bacterium was characterized as follows: Gram-negative, coccobacillus, facultative anaerobe, hemolytic, glucose and sucrose fermentative but not lactose and xylose, negative to catalase, Methyl red, Voges-Proskauer and hydrogen sulphide production tests, and positive to Simmon’s citrate utilization, liquefaction of gelatin, nitrate reduction, urea hydrolysis and indole production.  Two-tailed goldfish challenged with Bacteroides spp. showed clinical signs such as gasping for air, irritability, sloughing of scale, lesion and watery internal organs.


Keywords: Shubunkin goldfish, two-tailed goldfish, biochemical tests, Bacteroides

Downloads

Download data is not yet available.

Author Biography

Alvin T Reyes, College of Fisheries-Freshwater Aquaculture Center, Central Luzon State University, Science City of Muñoz, Nueva Ecija, Philippines

College of Fisheries-Freshwater Aquaculture Center, Central Luzon State University, Science City of Muñoz, Nueva Ecija, Philippines

References

1. Olivier K, World trade in ornamental species. In: Marine Ornamental Species, edited by Cato J., Brown C., Blackwell Publishing, Ames Iowa, 2003; pp. 49–64.
2. Magnadottir B, Innate immunity in fish (Overview). Fish Shellfish Immunol., 2006; 20:137-151.
3. Walczak N, Puk K, Guz L, Bacterial flora associated with diseased freshwater ornamental fish, J. Vet. Res., 2017; 61:445-449.
4. Lewbart G, Important infectious diseases of ornamental fish. Available at: https://www.vin.com/apputil/content/defaultadv1.aspx?id=3843980&pid=11131&print
5. Carassius auratus (Linnaeus, 1758)". Fishbase. Retrieved 2019-5-10.
6. Han S, Liu Y, Zhou Z, He S, Cao Y, Shi P, Yao B, Ring E, Analysis of bacterial diversity in the intestine of grass carp (Ctenopharyngodon idellus) based on 16S rDNA gene sequences, Aquaculture Research, 2010; 42:47-56, doi:10.1111/j.1365-2109.2010.02543.x.
7. Levinson W, Review of medical microbiology and immunology (11th ed.), 2010.
8. Gilmore MS, Ferretti JJ, The thin line between gut commensal and pathogen, Science, 2003; 299:1999-2002.
9. Tzianabos AO, Onderdonk AB, Rosner B, Cisneros RL, Kasper DL, Structural features of polysaccharides that induce intra-abdominal abscesses. Science, 1993; 262:416-419.
10. Gemmell CG, Peterson PK, Schmeling D, Mathews J, Quie PG, Antibiotic-induced modification of Bacteroides fragilis and its susceptibility to phagocytosis by human polymorphonuclear leukocytes, Eur. J. Clin. Microbiol., 1983; 2:327-334.
Statistics
54 Views | 97 Downloads
How to Cite
1.
Reyes AT. Two-tailed fancy goldfish challenged with Bacteroides spp. isolated from shubunkin goldfish. JDDT [Internet]. 15May2019 [cited 26May2020];9(3):360-5. Available from: http://jddtonline.info/index.php/jddt/article/view/2681
Issue
Vol 9 No 3 (2019): Volume 9, Issue 3, May-June 2019
Section
Research
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).